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recombinant mouse leptin  (R&D Systems)


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    R&D Systems recombinant mouse leptin
    Recombinant Mouse Leptin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+leptin/Recombinant+Mouse+Leptin+Protein%2C+CF/pm42017526-76-0-3
    Average 95 stars, based on 200 article reviews
    recombinant mouse leptin - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Injection:

    Article Title: Adoptive Transfer of Lepr + Bone Marrow Cells Attenuates the Osteopetrotic Phenotype of db / db Mice
    Article Snippet: .. Mouse leptin (498-OB-05M, R&D Systems, Minneapolis, MN) was delivered continuously (6 μg/d) using subcutaneously implanted osmotic pumps (Alzet Model 1002, Durect Corporation, Cupertino, CA, USA) in the ob / ob + cLeptin group or intermittently by subcutaneous daily injection (40 μg/d) in the ob / ob + iLeptin group. ..

    Article Title: Maternal emulsifier consumption programs offspring metabolic and neuropsychological health in mice.
    Article Snippet: .. Twelve-week-old mice from each experimental group were IP injected with either 5 μg/g of mouse leptin (R&D Systems) or vehicle 1 h before lights out (7 PM). ..

    Article Title: Adoptive Transfer of Lepr + Bone Marrow Cells Attenuates the Osteopetrotic Phenotype of db / db Mice.
    Article Snippet: .. Mouse leptin (498-OB-05M, R&D Systems, Minneapolis, MN) was delivered continuously (6μg/d) using subcutaneously implanted osmotic pumps (Alzet Model 1002, Durect Corporation, Cupertino, CA, USA) in the ob/ob + cLeptin group or intermittently by subcutaneous daily injection (40μg/d) in the ob/ob + iLeptin group. ..

    other:

    Article Title: Adoptive Transfer of Lepr + Bone Marrow Cells Attenuates the Osteopetrotic Phenotype of db / db Mice
    Article Snippet: Vehicle (20 mM Tris-HCL, Invitrogen, Carlsbad, CA, USA) or mouse leptin (498-OB-05M, R&D Systems, Minneapolis, MN) was infused using subcutaneously implanted osmotic pumps (Alzet Model 1002, Durect Corporation, Cupertino, CA, USA) for the 2-week duration of the study.

    Article Title: Adoptive Transfer of Lepr + Bone Marrow Cells Attenuates the Osteopetrotic Phenotype of db / db Mice.
    Article Snippet: Vehicle (20 mM Tris-HCL, Invitrogen, Carlsbad, CA, USA) or mouse leptin (498-OB-05M, R&D Systems, Minneapolis, MN) was infused using subcutaneously implanted osmotic pumps (Alzet Model 1002, Durect Corporation, Cupertino, CA, USA) for the 2-week duration of the study.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Carbon tetrachloride does not promote hepatic fibrosis in ob/ob mice via downregulation of lipocalin-2 protein
    Article Snippet: .. Serum leptin, insulin, and LCN2 concentrations were measured using mouse leptin (R&D Systems, Minneapolis, MN, USA), insulin (Shibayagi Co., Gunma, Japan), and LCN2 (R&D Systems) enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's protocols. ..

    Article Title: Adiponectin restores the obesity-induced impaired immunomodulatory function of mesenchymal stromal cells via glycolytic reprogramming
    Article Snippet: .. Serum adiponectin and leptin levels were measured in lean and obese mice using commercial ELISA kits for mouse adiponectin/Acrp30 (#DY1119) and mouse leptin (#DY498-05, R&D Systems (Minneapolis, MN, USA)). ..

    Article Title: Carbon tetrachloride does not promote hepatic fibrosis in ob/ob mice via downregulation of lipocalin-2 protein.
    Article Snippet: .. Serum leptin, insulin, and LCN2 concentrations were measured using mouse leptin (R&D Systems, Minneapolis, MN, USA), insulin (Shibayagi Co., Gunma, Japan), and LCN2 (R&D Systems) enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s protocols. ..



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    Vagotomy promoted Ly6G + cell infiltration into eWAT. Wild‐type mice were subjected to left cervical vagotomy (VX) or sham surgery and tissues were collected at 7 days following surgery. (A) The percentage of non‐adipocyte nuclei of total nuclei per section was quantified using ImageJ ( n = 4) and right panels show representative images of paraffin sections of eWAT stained with H&E in sham and VX animals. (B) <t>CCL2</t> release from eWAT was analyzed by ELISA. The bar shows the CCL2 levels from sham ( n = 4) or VX ( n = 4) mice normalized to eWAT weight: ng/mL per g ± SEM (unpaired Student's t test). (C) eWAT was collected at 1 ( n = 3), 4 ( n = 4 sham, n = 5 VX), and 7 ( n = 15) days following VX or sham surgery and the eWAT SVCs were analyzed by flow cytometry. The bar shows the % ± SEM of CD11b + Ly6G + cells from CD45 + (one‐way ANOVA, Uncorrected Fisher's LSD). (D) Graphs show representative gating for CD11b + Ly6G + cells in sham and VX eWAT at 7 days (concatenated n = 5–6). (E) Representative immunostaining of Ly6G (red) and Perilipin1 (green) in paraffin sections of eWAT. (F–H) Bone marrow neutrophils after sham ( n = 9) or VX ( n = 5) surgery were isolated using negative magnetic beads and analyzed using bulk RNAseq (DESeq2). Heatmap (F), volcano plot (G) of differentially expressed genes, and GO (Gene Ontology) (H) enrichment bar plot. ns = not significant, * p < 0.05. VX, Vagotomy; eWAT, epididymal white adipose tissue; H&E, hematoxylin–eosin; SVCs, stromal vascular cells.
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    recombinant mouse leptin 498 ob  (R&D Systems)
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    Vagotomy promoted Ly6G + cell infiltration into eWAT. Wild‐type mice were subjected to left cervical vagotomy (VX) or sham surgery and tissues were collected at 7 days following surgery. (A) The percentage of non‐adipocyte nuclei of total nuclei per section was quantified using ImageJ ( n = 4) and right panels show representative images of paraffin sections of eWAT stained with H&E in sham and VX animals. (B) <t>CCL2</t> release from eWAT was analyzed by ELISA. The bar shows the CCL2 levels from sham ( n = 4) or VX ( n = 4) mice normalized to eWAT weight: ng/mL per g ± SEM (unpaired Student's t test). (C) eWAT was collected at 1 ( n = 3), 4 ( n = 4 sham, n = 5 VX), and 7 ( n = 15) days following VX or sham surgery and the eWAT SVCs were analyzed by flow cytometry. The bar shows the % ± SEM of CD11b + Ly6G + cells from CD45 + (one‐way ANOVA, Uncorrected Fisher's LSD). (D) Graphs show representative gating for CD11b + Ly6G + cells in sham and VX eWAT at 7 days (concatenated n = 5–6). (E) Representative immunostaining of Ly6G (red) and Perilipin1 (green) in paraffin sections of eWAT. (F–H) Bone marrow neutrophils after sham ( n = 9) or VX ( n = 5) surgery were isolated using negative magnetic beads and analyzed using bulk RNAseq (DESeq2). Heatmap (F), volcano plot (G) of differentially expressed genes, and GO (Gene Ontology) (H) enrichment bar plot. ns = not significant, * p < 0.05. VX, Vagotomy; eWAT, epididymal white adipose tissue; H&E, hematoxylin–eosin; SVCs, stromal vascular cells.
    Recombinant Mouse Leptin 498 Ob, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Vagotomy promoted Ly6G + cell infiltration into eWAT. Wild‐type mice were subjected to left cervical vagotomy (VX) or sham surgery and tissues were collected at 7 days following surgery. (A) The percentage of non‐adipocyte nuclei of total nuclei per section was quantified using ImageJ ( n = 4) and right panels show representative images of paraffin sections of eWAT stained with H&E in sham and VX animals. (B) CCL2 release from eWAT was analyzed by ELISA. The bar shows the CCL2 levels from sham ( n = 4) or VX ( n = 4) mice normalized to eWAT weight: ng/mL per g ± SEM (unpaired Student's t test). (C) eWAT was collected at 1 ( n = 3), 4 ( n = 4 sham, n = 5 VX), and 7 ( n = 15) days following VX or sham surgery and the eWAT SVCs were analyzed by flow cytometry. The bar shows the % ± SEM of CD11b + Ly6G + cells from CD45 + (one‐way ANOVA, Uncorrected Fisher's LSD). (D) Graphs show representative gating for CD11b + Ly6G + cells in sham and VX eWAT at 7 days (concatenated n = 5–6). (E) Representative immunostaining of Ly6G (red) and Perilipin1 (green) in paraffin sections of eWAT. (F–H) Bone marrow neutrophils after sham ( n = 9) or VX ( n = 5) surgery were isolated using negative magnetic beads and analyzed using bulk RNAseq (DESeq2). Heatmap (F), volcano plot (G) of differentially expressed genes, and GO (Gene Ontology) (H) enrichment bar plot. ns = not significant, * p < 0.05. VX, Vagotomy; eWAT, epididymal white adipose tissue; H&E, hematoxylin–eosin; SVCs, stromal vascular cells.

    Journal: The FASEB Journal

    Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight

    doi: 10.1096/fj.202600151RR

    Figure Lengend Snippet: Vagotomy promoted Ly6G + cell infiltration into eWAT. Wild‐type mice were subjected to left cervical vagotomy (VX) or sham surgery and tissues were collected at 7 days following surgery. (A) The percentage of non‐adipocyte nuclei of total nuclei per section was quantified using ImageJ ( n = 4) and right panels show representative images of paraffin sections of eWAT stained with H&E in sham and VX animals. (B) CCL2 release from eWAT was analyzed by ELISA. The bar shows the CCL2 levels from sham ( n = 4) or VX ( n = 4) mice normalized to eWAT weight: ng/mL per g ± SEM (unpaired Student's t test). (C) eWAT was collected at 1 ( n = 3), 4 ( n = 4 sham, n = 5 VX), and 7 ( n = 15) days following VX or sham surgery and the eWAT SVCs were analyzed by flow cytometry. The bar shows the % ± SEM of CD11b + Ly6G + cells from CD45 + (one‐way ANOVA, Uncorrected Fisher's LSD). (D) Graphs show representative gating for CD11b + Ly6G + cells in sham and VX eWAT at 7 days (concatenated n = 5–6). (E) Representative immunostaining of Ly6G (red) and Perilipin1 (green) in paraffin sections of eWAT. (F–H) Bone marrow neutrophils after sham ( n = 9) or VX ( n = 5) surgery were isolated using negative magnetic beads and analyzed using bulk RNAseq (DESeq2). Heatmap (F), volcano plot (G) of differentially expressed genes, and GO (Gene Ontology) (H) enrichment bar plot. ns = not significant, * p < 0.05. VX, Vagotomy; eWAT, epididymal white adipose tissue; H&E, hematoxylin–eosin; SVCs, stromal vascular cells.

    Article Snippet: In vitro release rate was calculated as mg NEFAs per mg eWAT tissue per hour. (2) CCL2 levels were quantified using a mouse CCL2 ELISA kit (R&D Systems, #DY497‐05), according to the manufacturer's instructions ( n = 1 experiment).

    Techniques: Staining, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunostaining, Isolation, Magnetic Beads, RNA sequencing