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hil 7  (R&D Systems)


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    R&D Systems hil 7
    Hil 7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il-7/Recombinant+Mouse+IL-7+Protein/pmc13059316-49-29-30
    Average 95 stars, based on 136 article reviews
    hil 7 - by Bioz Stars, 2026-09
    95/100 stars

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    Purification:

    Article Title: Interleukin-7 matures suppressive CD127 + forkhead box P3 (FoxP3) + T cells into CD127 − CD25 high FoxP3 + regulatory T cells
    Article Snippet: T cells were left unstimulated, or where stimulation was required they were treated with either 50 ng/ml mouse IL-2 (R&D Systems) or 50 ng/ml mouse IL-7 (R&D Systems) for the indicated time-periods prior to further manipulations/analyses.

    Article Title: Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
    Article Snippet: Thymocytes (5 104) from triplicate cultures were stimulated for 24 h with either concanavalin A (ConA; 20 g/ml), ConA plus mouse interleukin-2 (IL-2; 10 U/ml; R & D Systems), ConA plus mouse IL-7 (5 ng/ml; R & D Systems), anti-CD3ε antibody (clone 145-2C11; 10 g/ml) plus phorbol 12-myristate 13-acetate (PMA; 10 ng/ml), or anti-CD3ε plus PMA plus IL-7, in the presence of 10% knockout serum replacement (KSR; Invitrogen) instead of fetal bovine serum (FBS), and pulsed with 0.5 Ci of [3H]thymidine for 14 h, after which the incorporation of [3H]thymidine was measured.

    Article Title: Stromal cells modulate ephrinB2 expression and transmigration of hematopoietic cells.
    Article Snippet: Objective.. Ephrin ligands and Eph receptors play important roles for cell behavior and movement by transducing bidirectional signaling into interacting cells.. Since we found that the expression of ephrinB2 in the hematopoietic progenitor cell line was changed by coculture with stromal cells, we tried to examine the function of ephrinB2 in the hematopoietic microenvironment.

    Article Title: In vitro-expanded donor alloantigen-specific CD4+CD25+ regulatory T cells promote experimental transplantation tolerance.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/109/2/827.full.html Updated information and services can be found at: (1815 articles) Transplantation (4871 articles) Immunobiology (1528 articles) Free Research Articles Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:

    Article Title: Chronically stimulated mouse invariant NKT cell lines have a preserved capacity to enhance protection against experimental tumor metastases.
    Article Snippet: In pre-clinical models, CD anti-tumor immune respo swift and potent cytokine circulating iNKT cell poo cell carcinoma.. Therefore the increase of circulating iNKT cell lines, and demo cytokines even after longcells (DC).. Here, we establ Tumor immunity cell lines we generated have a pr independently of supplementa protection against B16.F10 expe of autologous adoptive transfer modalities for the treatment of

    Crocin Bleaching Assay:

    Article Title: Interleukin-7 matures suppressive CD127 + forkhead box P3 (FoxP3) + T cells into CD127 − CD25 high FoxP3 + regulatory T cells
    Article Snippet: T cells were left unstimulated, or where stimulation was required they were treated with either 50 ng/ml mouse IL-2 (R&D Systems) or 50 ng/ml mouse IL-7 (R&D Systems) for the indicated time-periods prior to further manipulations/analyses.

    Article Title: Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
    Article Snippet: Thymocytes (5 104) from triplicate cultures were stimulated for 24 h with either concanavalin A (ConA; 20 g/ml), ConA plus mouse interleukin-2 (IL-2; 10 U/ml; R & D Systems), ConA plus mouse IL-7 (5 ng/ml; R & D Systems), anti-CD3ε antibody (clone 145-2C11; 10 g/ml) plus phorbol 12-myristate 13-acetate (PMA; 10 ng/ml), or anti-CD3ε plus PMA plus IL-7, in the presence of 10% knockout serum replacement (KSR; Invitrogen) instead of fetal bovine serum (FBS), and pulsed with 0.5 Ci of [3H]thymidine for 14 h, after which the incorporation of [3H]thymidine was measured.

    Article Title: Stromal cells modulate ephrinB2 expression and transmigration of hematopoietic cells.
    Article Snippet: Objective.. Ephrin ligands and Eph receptors play important roles for cell behavior and movement by transducing bidirectional signaling into interacting cells.. Since we found that the expression of ephrinB2 in the hematopoietic progenitor cell line was changed by coculture with stromal cells, we tried to examine the function of ephrinB2 in the hematopoietic microenvironment.

    Article Title: In vitro-expanded donor alloantigen-specific CD4+CD25+ regulatory T cells promote experimental transplantation tolerance.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/109/2/827.full.html Updated information and services can be found at: (1815 articles) Transplantation (4871 articles) Immunobiology (1528 articles) Free Research Articles Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:

    Article Title: Chronically stimulated mouse invariant NKT cell lines have a preserved capacity to enhance protection against experimental tumor metastases.
    Article Snippet: In pre-clinical models, CD anti-tumor immune respo swift and potent cytokine circulating iNKT cell poo cell carcinoma.. Therefore the increase of circulating iNKT cell lines, and demo cytokines even after longcells (DC).. Here, we establ Tumor immunity cell lines we generated have a pr independently of supplementa protection against B16.F10 expe of autologous adoptive transfer modalities for the treatment of

    Cell Culture:

    Article Title: Interleukin-7 matures suppressive CD127 + forkhead box P3 (FoxP3) + T cells into CD127 − CD25 high FoxP3 + regulatory T cells
    Article Snippet: T cells were left unstimulated, or where stimulation was required they were treated with either 50 ng/ml mouse IL-2 (R&D Systems) or 50 ng/ml mouse IL-7 (R&D Systems) for the indicated time-periods prior to further manipulations/analyses.

    Article Title: Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
    Article Snippet: Thymocytes (5 104) from triplicate cultures were stimulated for 24 h with either concanavalin A (ConA; 20 g/ml), ConA plus mouse interleukin-2 (IL-2; 10 U/ml; R & D Systems), ConA plus mouse IL-7 (5 ng/ml; R & D Systems), anti-CD3ε antibody (clone 145-2C11; 10 g/ml) plus phorbol 12-myristate 13-acetate (PMA; 10 ng/ml), or anti-CD3ε plus PMA plus IL-7, in the presence of 10% knockout serum replacement (KSR; Invitrogen) instead of fetal bovine serum (FBS), and pulsed with 0.5 Ci of [3H]thymidine for 14 h, after which the incorporation of [3H]thymidine was measured.

    Article Title: Stromal cells modulate ephrinB2 expression and transmigration of hematopoietic cells.
    Article Snippet: Objective.. Ephrin ligands and Eph receptors play important roles for cell behavior and movement by transducing bidirectional signaling into interacting cells.. Since we found that the expression of ephrinB2 in the hematopoietic progenitor cell line was changed by coculture with stromal cells, we tried to examine the function of ephrinB2 in the hematopoietic microenvironment.

    Article Title: In vitro-expanded donor alloantigen-specific CD4+CD25+ regulatory T cells promote experimental transplantation tolerance.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/109/2/827.full.html Updated information and services can be found at: (1815 articles) Transplantation (4871 articles) Immunobiology (1528 articles) Free Research Articles Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:

    Article Title: Chronically stimulated mouse invariant NKT cell lines have a preserved capacity to enhance protection against experimental tumor metastases.
    Article Snippet: In pre-clinical models, CD anti-tumor immune respo swift and potent cytokine circulating iNKT cell poo cell carcinoma.. Therefore the increase of circulating iNKT cell lines, and demo cytokines even after longcells (DC).. Here, we establ Tumor immunity cell lines we generated have a pr independently of supplementa protection against B16.F10 expe of autologous adoptive transfer modalities for the treatment of

    Irradiation:

    Article Title: Interleukin-7 matures suppressive CD127 + forkhead box P3 (FoxP3) + T cells into CD127 − CD25 high FoxP3 + regulatory T cells
    Article Snippet: T cells were left unstimulated, or where stimulation was required they were treated with either 50 ng/ml mouse IL-2 (R&D Systems) or 50 ng/ml mouse IL-7 (R&D Systems) for the indicated time-periods prior to further manipulations/analyses.

    Article Title: Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
    Article Snippet: Thymocytes (5 104) from triplicate cultures were stimulated for 24 h with either concanavalin A (ConA; 20 g/ml), ConA plus mouse interleukin-2 (IL-2; 10 U/ml; R & D Systems), ConA plus mouse IL-7 (5 ng/ml; R & D Systems), anti-CD3ε antibody (clone 145-2C11; 10 g/ml) plus phorbol 12-myristate 13-acetate (PMA; 10 ng/ml), or anti-CD3ε plus PMA plus IL-7, in the presence of 10% knockout serum replacement (KSR; Invitrogen) instead of fetal bovine serum (FBS), and pulsed with 0.5 Ci of [3H]thymidine for 14 h, after which the incorporation of [3H]thymidine was measured.

    Article Title: Stromal cells modulate ephrinB2 expression and transmigration of hematopoietic cells.
    Article Snippet: Objective.. Ephrin ligands and Eph receptors play important roles for cell behavior and movement by transducing bidirectional signaling into interacting cells.. Since we found that the expression of ephrinB2 in the hematopoietic progenitor cell line was changed by coculture with stromal cells, we tried to examine the function of ephrinB2 in the hematopoietic microenvironment.

    Article Title: In vitro-expanded donor alloantigen-specific CD4+CD25+ regulatory T cells promote experimental transplantation tolerance.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/109/2/827.full.html Updated information and services can be found at: (1815 articles) Transplantation (4871 articles) Immunobiology (1528 articles) Free Research Articles Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:

    Article Title: Chronically stimulated mouse invariant NKT cell lines have a preserved capacity to enhance protection against experimental tumor metastases.
    Article Snippet: In pre-clinical models, CD anti-tumor immune respo swift and potent cytokine circulating iNKT cell poo cell carcinoma.. Therefore the increase of circulating iNKT cell lines, and demo cytokines even after longcells (DC).. Here, we establ Tumor immunity cell lines we generated have a pr independently of supplementa protection against B16.F10 expe of autologous adoptive transfer modalities for the treatment of

    Sequencing:

    Article Title: Interleukin-7 matures suppressive CD127 + forkhead box P3 (FoxP3) + T cells into CD127 − CD25 high FoxP3 + regulatory T cells
    Article Snippet: T cells were left unstimulated, or where stimulation was required they were treated with either 50 ng/ml mouse IL-2 (R&D Systems) or 50 ng/ml mouse IL-7 (R&D Systems) for the indicated time-periods prior to further manipulations/analyses.

    Article Title: Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
    Article Snippet: Thymocytes (5 104) from triplicate cultures were stimulated for 24 h with either concanavalin A (ConA; 20 g/ml), ConA plus mouse interleukin-2 (IL-2; 10 U/ml; R & D Systems), ConA plus mouse IL-7 (5 ng/ml; R & D Systems), anti-CD3ε antibody (clone 145-2C11; 10 g/ml) plus phorbol 12-myristate 13-acetate (PMA; 10 ng/ml), or anti-CD3ε plus PMA plus IL-7, in the presence of 10% knockout serum replacement (KSR; Invitrogen) instead of fetal bovine serum (FBS), and pulsed with 0.5 Ci of [3H]thymidine for 14 h, after which the incorporation of [3H]thymidine was measured.

    Article Title: Stromal cells modulate ephrinB2 expression and transmigration of hematopoietic cells.
    Article Snippet: Objective.. Ephrin ligands and Eph receptors play important roles for cell behavior and movement by transducing bidirectional signaling into interacting cells.. Since we found that the expression of ephrinB2 in the hematopoietic progenitor cell line was changed by coculture with stromal cells, we tried to examine the function of ephrinB2 in the hematopoietic microenvironment.

    Article Title: In vitro-expanded donor alloantigen-specific CD4+CD25+ regulatory T cells promote experimental transplantation tolerance.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/109/2/827.full.html Updated information and services can be found at: (1815 articles) Transplantation (4871 articles) Immunobiology (1528 articles) Free Research Articles Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:

    Article Title: Chronically stimulated mouse invariant NKT cell lines have a preserved capacity to enhance protection against experimental tumor metastases.
    Article Snippet: In pre-clinical models, CD anti-tumor immune respo swift and potent cytokine circulating iNKT cell poo cell carcinoma.. Therefore the increase of circulating iNKT cell lines, and demo cytokines even after longcells (DC).. Here, we establ Tumor immunity cell lines we generated have a pr independently of supplementa protection against B16.F10 expe of autologous adoptive transfer modalities for the treatment of



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    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured <t>with</t> <t>IL-7</t> for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
    Recombinant Mouse Il 7 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured <t>with</t> <t>IL-7</t> for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
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    Image Search Results


    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer

    doi: 10.64898/2026.04.21.720007

    Figure Lengend Snippet: (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Article Snippet: 7.5 x 10 5 cells/ml were cultured in IMDM media containing Glutamax (Gibco, 31980030) supplemented with 10% FBS, 1 × P/S and 5μM 2-Mercaptoethanol, and 10 ng/ml of recombinant mouse IL-7 (Invitrogen, RP-8664) in a humidified incubator at 37 °C and 5% CO 2 .

    Techniques: Injection, Colony-forming Unit Assay, Cell Culture, Two Tailed Test