mouse fibroblast cell line (ATCC)

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ATCC mouse fibroblast cell line
Mouse Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fibroblast+l+cells/L+Cells%3B+Fibroblast%3B+Mouse/10__3390_slash_ph19030356-215-5-10
Average 96 stars, based on 442 article reviews

mouse fibroblast cell line - by Bioz Stars, 2026-11

96/100 stars

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Article Title: Efficient production of inhibitor-free foamy virus glycoprotein-containing retroviral vectors by proteoglycan-deficient packaging cells
Article Snippet: .. The human embryonic kidney cell line 293T (ATCC CRL-1573, ) as well as its HS-deficient variants 293T-25A and 293T-306 generated in this study and described below (see ); the human epithelial fibrosarcoma cell line HT1080 (ATCC CCL-121 ) as well as the clonal variant HT1080 PLNE thereof containing a PFV LTR-driven EGFP reporter gene expression cassette; and mouse fibroblast L cells (ATCC CRL-264, ) as well as its variant Sog9 with a defect in initiation of GAG assembly so that no surface HS is being produced (gift of F. Neipel, Erlangen ) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum and antibiotics. .. The human T lymphocyte Jurkat cell line (ATCC TIB-152, ) and the human monocyte cell line THP-1 (ATCC TIB-202, ) were cultivated in RPMI supplemented with 10% heat-inactivated fetal calf serum, 1% non-essential amino acids, 1% sodium pyruvate, and 1% L-alanine, L-glutamate.

Variant Assay:

Gene Expression:

Produced:

Modification:

Article Title: Identification of genetic elements required for Listeria monocytogenes growth under limited nutrient conditions and virulence by a screening of transposon insertion library
Article Snippet: .. Mouse fibroblast L cells (American Type Culture Collection, Manassas, Virginia) were propagated in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS). ..

Article Title: Differences in a Single Extracellular Residue Underlie Adhesive Functions of Two Zebrafish Aqp0s
Article Snippet: .. Mouse fibroblast L cells (ATCC cell bank, Manassas, VA, USA) were cultured under standard culture conditions of 5% CO 2 at 37 °C in Dulbecco’s modified Eagle’s minimum essential medium (DMEM; Nacalai Tesque, Kyoto, Japan) with a penicillin–streptomycin antibiotic mixture (100 U/mL and 100 mg/mL, respectively) containing 10% fetal calf serum (FBS; Life Technologies, Waltham, CA, USA). ..

Article Title: FAM83F regulates canonical Wnt signalling through an interaction with CK1α
Article Snippet: Blots were then imaged using the LI-COR Odyssey imaging system in 700 and 800 nm channels and processed for brightness and contrast in Adobe Photoshop CC (2019). .. U2OS (HTB-96, ATCC), HCT116 (CCL-247, ATCC), DLD-1 (CCL-221, ATCC), mouse fibroblast L-cells that stably overexpress Wnt3A (CRL-2647, ATCC), mouse fibroblast L cells (CRL-2648, ATCC), Flp-In T-Rex U2OS (which were created using Flp-In T-Rex Core kit (K650001, Thermo Fisher Scientific) and have been previously reported ( )), Flp-In T-Rex HEK293 (R78007, Thermo Fisher Scientific) and HaCaT (obtained from Joan Massague’s lab at Memorial Sloan Kettering Cancer Centre, not commercially available but can be provided on request) ( ) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) containing 10 % FCS (Hyclone), 100 U/ml penicillin (Lonza), 100 mg/ml streptomycin (Lonza) and 2 mM L-glutamine (Lonza). ..

other:

Article Title: CAR T cells targeting BAFF-R can overcome CD19 antigen loss in B cell malignancies.
Article Snippet: Mouse fibroblast L cells, HT1080 human epithelial cells, and 293FT cells were obtained from the ATCC.

Stable Transfection:

Article Title: FAM83F regulates canonical Wnt signalling through an interaction with CK1α
Article Snippet: Blots were then imaged using the LI-COR Odyssey imaging system in 700 and 800 nm channels and processed for brightness and contrast in Adobe Photoshop CC (2019). .. U2OS (HTB-96; ATCC), HCT116 (CCL-247; ATCC), DLD-1 (CCL-221; ATCC), mouse fibroblast L-cells that stably overexpress Wnt3A (CRL-2647; ATCC), mouse fibroblast L cells (CRL-2648; ATCC), Flp-In T-Rex U2OS (which were created using Flp-In T-Rex Core kit [K650001; Thermo Fisher Scientific] and have been previously reported ( )), Flp-In T-Rex HEK293 (R78007; Thermo Fisher Scientific) and HaCaT (obtained from Joan Massague’s lab at Memorial Sloan Kettering Cancer Centre, not commercially available but can be provided on request) ( ) cells were maintained in DMEM (Gibco) containing 10% FCS (Hyclone), 100 U/ml penicillin (Lonza), 100 mg/ml streptomycin (Lonza), and 2 mM L-glutamine (Lonza). ..

Cell Culture:

Article Title: Peptide-based inhibitors of growth hormone action and methods of use thereof
Article Snippet: Data were processed using J-700® Software version 1.5 (Jasco) and KaleidaGraph® version 4.5 (Synergy Software). .. Cell Culture and hGH Treatment Human malignant melanoma cell lines—SK-MEL-28, SK-MEL-30, human lymphoblast IM9 cells, mouse fibroblast L cells were obtained from American Type Culture Collection (ATCC; Manassas, Va.). .. SK-MEL-28 were grown and maintained in EMEM media (ATCC), while IM9 and L cells were grown in RPMI-1640 (ATCC) and DMEM (ATCC) respectively, as indicated by ATCC protocols.

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