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anti mouse dectin 1 apc antibody  (Miltenyi Biotec)


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    Miltenyi Biotec anti mouse dectin 1 apc antibody
    Anti Mouse Dectin 1 Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+dectin/Dectin-1+Antibody%2C+anti-mouse%2C+REAfinity/pm41916428-87-21-25
    Average 93 stars, based on 13 article reviews
    anti mouse dectin 1 apc antibody - by Bioz Stars, 2026-11
    93/100 stars

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    Incubation:

    Article Title: Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances
    Article Snippet: .. The sections were incubated overnight at 4°C in a dark environment with the following antibodies: anti-cytokeratin 10-REAfinityTM, anti-cytokeratin 14 REAfinityTM, anti-vimentin (all three from Miltenyi Biotech, Bergisch Gladbach, Germany), anti-filaggrin, anti-claudin, or anti-involucrin (all three from Invitrogen, Carlsbad, USA), anti-CD209, anti-HLA-DR, anti-Dectin-1 (all three from Miltenyi Biotech, Bergisch Gladbach, Germany). ..

    Article Title: Basal phosphorylation of SHIP1 by Lyn suppresses proinflammatory signaling in the absence of a phagocytic synapse
    Article Snippet: .. Following β-glucan treatment, cells were incubated with a 1:10 dilution of anti-mouse Dectin-1-APC antibody (130-102-250; Miltenyi Biotec). ..

    Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse
    Article Snippet: .. Following β-glucan treatment, cells were incubated with a 1:10 dilution of anti-mouse Dectin-1-APC antibody (Cat# 130-102-250, Miltenyi Biotec, Gaithersburg, MD, USA). .. Binding was assessed by measuring the decrease in gMFI values compared to untreated BMDMs. gMFI values were corrected for background fluorescence and plotted against the corresponding β-glucan concentration using GraphPad Prism (San Diego, CA).

    Article Title: Basal phosphorylation of SHIP1 by Lyn suppresses proinflammatory signaling in the absence of a phagocytic synapse.
    Article Snippet: .. D ow nloaded from https://academ ic.oup.com /jim m unol/article/215/3/vkaf372/8566561 by guest on 03 April 2026 incubated with a 1:10 dilution of anti-mouse Dectin-1-APC antibody (130-102-250; Miltenyi Biotec). ..

    Centrifugation:

    Article Title: Identification and structural characterization of soluble dectin-1 with β-glucan-binding activity in THP-1 cell culture supernatant.
    Article Snippet: .. THP-1 cells were harvested by centrifugation (300 ×g, 5 min), washed with FACS buffer (PBS + 2% FBS + 0.1% NaN3), and stained on ice for 30 min with anti-human Dectin-1 Vio® Bright FITC REAfinityTM antibody (Miltenyi Biotec, Cat130–107-693) or isotype control (Miltenyi Biotec, Cat#130–113-455) at the manufacturer's recommended dilution. .. The cells were washed twice, fixed in 10% neutral-buffered formalin for 15 min, and analyzed using a FACSCelesta flow cytometer (BD Biosciences) and FlowJo software (v10.8).

    FACS:

    Article Title: Identification and structural characterization of soluble dectin-1 with β-glucan-binding activity in THP-1 cell culture supernatant.
    Article Snippet: .. THP-1 cells were harvested by centrifugation (300 ×g, 5 min), washed with FACS buffer (PBS + 2% FBS + 0.1% NaN3), and stained on ice for 30 min with anti-human Dectin-1 Vio® Bright FITC REAfinityTM antibody (Miltenyi Biotec, Cat130–107-693) or isotype control (Miltenyi Biotec, Cat#130–113-455) at the manufacturer's recommended dilution. .. The cells were washed twice, fixed in 10% neutral-buffered formalin for 15 min, and analyzed using a FACSCelesta flow cytometer (BD Biosciences) and FlowJo software (v10.8).

    Staining:

    Article Title: Identification and structural characterization of soluble dectin-1 with β-glucan-binding activity in THP-1 cell culture supernatant.
    Article Snippet: .. THP-1 cells were harvested by centrifugation (300 ×g, 5 min), washed with FACS buffer (PBS + 2% FBS + 0.1% NaN3), and stained on ice for 30 min with anti-human Dectin-1 Vio® Bright FITC REAfinityTM antibody (Miltenyi Biotec, Cat130–107-693) or isotype control (Miltenyi Biotec, Cat#130–113-455) at the manufacturer's recommended dilution. .. The cells were washed twice, fixed in 10% neutral-buffered formalin for 15 min, and analyzed using a FACSCelesta flow cytometer (BD Biosciences) and FlowJo software (v10.8).

    Article Title: Murine peritoneal macrophages undergo female-specific remodeling with aging
    Article Snippet: To estimate potential differences in peritoneal macrophage surface markers ( i.e . Zymosan pattern recognition receptors, M1/M2 markers) with respect to sex and age, 100,000 MACS-purified peritoneal macrophages were subjected to Fc receptor blocking (Miltenyi Biotec #130-092-575) and stained with corresponding antibodies according to the manufacturer’s instructions. .. For analysis of pattern recognition receptors, cells were stained with F4/80-Brilliant Violet 421 (1:20) (Biolegend #123131), Dectin-1-PE (1:10) (Miltenyi Biotec #130-102-284), and CD282 (Tlr2)-APC (1:50) (Miltenyi Biotec #130-120-052). .. For M1 marker detection, cells were stained with CD80-FITC (1:20) (Biolegend #104705) and CD86-APC (1:20) (Biolegend #105011).

    Control:

    Article Title: Identification and structural characterization of soluble dectin-1 with β-glucan-binding activity in THP-1 cell culture supernatant.
    Article Snippet: .. THP-1 cells were harvested by centrifugation (300 ×g, 5 min), washed with FACS buffer (PBS + 2% FBS + 0.1% NaN3), and stained on ice for 30 min with anti-human Dectin-1 Vio® Bright FITC REAfinityTM antibody (Miltenyi Biotec, Cat130–107-693) or isotype control (Miltenyi Biotec, Cat#130–113-455) at the manufacturer's recommended dilution. .. The cells were washed twice, fixed in 10% neutral-buffered formalin for 15 min, and analyzed using a FACSCelesta flow cytometer (BD Biosciences) and FlowJo software (v10.8).

    Article Title: Innate lymphoid cells integrate sensing and plasticity to control fungal infections
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human IgG1 Recombinant Isotype Control (REA293) Invitrogen; Fisher Scient Cat#MA555092 CD11b, anti-mouse, Biotin, Clone REA592 Miltenyi Cat#130-113-804, RRID:AB_2733498 CD11b, anti-mouse, V500, Clone M1/70 BD Cat#562127, RRID:AB_10893815 CD11c, anti-mouse, Biotin, Clone REA754 Miltenyi Cat#130-110-699, RRID:AB_2654719 CD11c, anti-mouse, APC, Clone HL3 BD Cat#550261, RRID:AB_398460 TCRβ, anti-mouse, Biotin, REAfinity Miltenyi Cat#130-125-217, RRID:AB_2889764 TCRβ, anti-mouse, Brilliant Violet 711, Clone H57-597 Biolegend Cat#109243, RRID:AB_2629564 TCRγ/δ, anti-mouse, Biotin, REAfinity Miltenyi Cat#130-109-795, RRID:AB_2654057 TCRγ/δ, anti-mouse, PE, Clone GL3 Biolegend Cat#118108, RRID:AB_313832 CD3, anti-mouse, Biotin, Clone REA641 Miltenyi Cat#130-123-861, RRID:AB_2819528 CD19, anti-mouse, Biotin, Clone REA749 Miltenyi Cat#130-111-882, RRID:AB_2655837 CD49b, anti-mouse, Biotin, Clone REA541 Miltenyi Cat#130-108-199, RRID;AB_2658477 CD282 (TLR2), anti-human/mouse, APC, Clone REA109 Miltenyi Cat#130-120-052, RRID:AB_2751976 CD282 (TLR2), anti-human/mouse, functional grade, Clone REA109 Miltenyi Cat#130-098-855, RRID:AB_2661336 Dectin-1, anti-mouse, PE-Vio770, Clone REA154 Miltenyi Cat#130-102-989, RRID:AB_2651545 Dectin-1, anti-mouse, functional grade Invivogen Cat#mabg-mdect-2, RRID:AB_2753143 Dectin-2, anti-mouse, rabbit (host), Clone M-45 Santa-Cruz Cat#Sc-134906, RRID:AB_10610962 Mincle, anti-mouse, functional grade Invivogen Cat#mabg-mmcl-2, RRID: AB_3741008 Rat IgG2a Control Invivogen Cat#mabg2a-ctlrt, RRID:AB_11124905 Rat IgG2b Control Invivogen Cat#mabg2b-ctlrt, RRID: AB_3741009 Goat anti-rabbit IgG F(ab’), PE Santa-Cruz Cat#Sc-3843, RRID:AB_649073 Anti-rabbit IgG Fab2, Alexa Fluor 594 Cell Signaling Technology Cat#8889S, RRID:AB_2716249 anti-Candida, polyclonal, rabbit (host) Seiser et al.113 N/A CD90.2 (Thy1.2), anti-mouse, PerCP/ Cyanine5.5, Clone 30-H12 Biolegend Cat#105337, RRID:AB_2571944 CD90.2 (Thy1.2), anti-mouse, Brilliant Violet 785, Clone 30-H12 Biolegend Cat#105331, RRID:AB_2562900 CD90.2 (Thy1.2), anti-mouse, Super Bright 702, Clone 53–2.1 eBioscience; Fisher Scient. .. Cat#67-0902-80, RRID:AB_2717156 CD335 (NKp46/NCR1), anti-mouse, PerCP/Fire 780, Clone 29A1.4 Biolegend Cat#137651, RRID:AB_3083275 TruStain FcX PLUS (anti-mouse CD16/32), Clone S17011E Biolegend Cat#156603, RRID:AB_2783137 CD127 (IL-7Ralpha), anti-mouse, PE, Clone A7R34 Biolegend Cat#135009, RRID:AB_1937252 CD107a (LAMP-1), anti-mouse, PE, Clone 1D4B Biolegend Cat#121612, RRID:AB_10455386 NK1.1, anti-mouse, PE-eFluor 610, Clone PK136 eBioscience; Fisher Scient.

    Recombinant:

    Article Title: Innate lymphoid cells integrate sensing and plasticity to control fungal infections
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human IgG1 Recombinant Isotype Control (REA293) Invitrogen; Fisher Scient Cat#MA555092 CD11b, anti-mouse, Biotin, Clone REA592 Miltenyi Cat#130-113-804, RRID:AB_2733498 CD11b, anti-mouse, V500, Clone M1/70 BD Cat#562127, RRID:AB_10893815 CD11c, anti-mouse, Biotin, Clone REA754 Miltenyi Cat#130-110-699, RRID:AB_2654719 CD11c, anti-mouse, APC, Clone HL3 BD Cat#550261, RRID:AB_398460 TCRβ, anti-mouse, Biotin, REAfinity Miltenyi Cat#130-125-217, RRID:AB_2889764 TCRβ, anti-mouse, Brilliant Violet 711, Clone H57-597 Biolegend Cat#109243, RRID:AB_2629564 TCRγ/δ, anti-mouse, Biotin, REAfinity Miltenyi Cat#130-109-795, RRID:AB_2654057 TCRγ/δ, anti-mouse, PE, Clone GL3 Biolegend Cat#118108, RRID:AB_313832 CD3, anti-mouse, Biotin, Clone REA641 Miltenyi Cat#130-123-861, RRID:AB_2819528 CD19, anti-mouse, Biotin, Clone REA749 Miltenyi Cat#130-111-882, RRID:AB_2655837 CD49b, anti-mouse, Biotin, Clone REA541 Miltenyi Cat#130-108-199, RRID;AB_2658477 CD282 (TLR2), anti-human/mouse, APC, Clone REA109 Miltenyi Cat#130-120-052, RRID:AB_2751976 CD282 (TLR2), anti-human/mouse, functional grade, Clone REA109 Miltenyi Cat#130-098-855, RRID:AB_2661336 Dectin-1, anti-mouse, PE-Vio770, Clone REA154 Miltenyi Cat#130-102-989, RRID:AB_2651545 Dectin-1, anti-mouse, functional grade Invivogen Cat#mabg-mdect-2, RRID:AB_2753143 Dectin-2, anti-mouse, rabbit (host), Clone M-45 Santa-Cruz Cat#Sc-134906, RRID:AB_10610962 Mincle, anti-mouse, functional grade Invivogen Cat#mabg-mmcl-2, RRID: AB_3741008 Rat IgG2a Control Invivogen Cat#mabg2a-ctlrt, RRID:AB_11124905 Rat IgG2b Control Invivogen Cat#mabg2b-ctlrt, RRID: AB_3741009 Goat anti-rabbit IgG F(ab’), PE Santa-Cruz Cat#Sc-3843, RRID:AB_649073 Anti-rabbit IgG Fab2, Alexa Fluor 594 Cell Signaling Technology Cat#8889S, RRID:AB_2716249 anti-Candida, polyclonal, rabbit (host) Seiser et al.113 N/A CD90.2 (Thy1.2), anti-mouse, PerCP/ Cyanine5.5, Clone 30-H12 Biolegend Cat#105337, RRID:AB_2571944 CD90.2 (Thy1.2), anti-mouse, Brilliant Violet 785, Clone 30-H12 Biolegend Cat#105331, RRID:AB_2562900 CD90.2 (Thy1.2), anti-mouse, Super Bright 702, Clone 53–2.1 eBioscience; Fisher Scient. .. Cat#67-0902-80, RRID:AB_2717156 CD335 (NKp46/NCR1), anti-mouse, PerCP/Fire 780, Clone 29A1.4 Biolegend Cat#137651, RRID:AB_3083275 TruStain FcX PLUS (anti-mouse CD16/32), Clone S17011E Biolegend Cat#156603, RRID:AB_2783137 CD127 (IL-7Ralpha), anti-mouse, PE, Clone A7R34 Biolegend Cat#135009, RRID:AB_1937252 CD107a (LAMP-1), anti-mouse, PE, Clone 1D4B Biolegend Cat#121612, RRID:AB_10455386 NK1.1, anti-mouse, PE-eFluor 610, Clone PK136 eBioscience; Fisher Scient.

    Functional Assay:

    Article Title: Innate lymphoid cells integrate sensing and plasticity to control fungal infections
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human IgG1 Recombinant Isotype Control (REA293) Invitrogen; Fisher Scient Cat#MA555092 CD11b, anti-mouse, Biotin, Clone REA592 Miltenyi Cat#130-113-804, RRID:AB_2733498 CD11b, anti-mouse, V500, Clone M1/70 BD Cat#562127, RRID:AB_10893815 CD11c, anti-mouse, Biotin, Clone REA754 Miltenyi Cat#130-110-699, RRID:AB_2654719 CD11c, anti-mouse, APC, Clone HL3 BD Cat#550261, RRID:AB_398460 TCRβ, anti-mouse, Biotin, REAfinity Miltenyi Cat#130-125-217, RRID:AB_2889764 TCRβ, anti-mouse, Brilliant Violet 711, Clone H57-597 Biolegend Cat#109243, RRID:AB_2629564 TCRγ/δ, anti-mouse, Biotin, REAfinity Miltenyi Cat#130-109-795, RRID:AB_2654057 TCRγ/δ, anti-mouse, PE, Clone GL3 Biolegend Cat#118108, RRID:AB_313832 CD3, anti-mouse, Biotin, Clone REA641 Miltenyi Cat#130-123-861, RRID:AB_2819528 CD19, anti-mouse, Biotin, Clone REA749 Miltenyi Cat#130-111-882, RRID:AB_2655837 CD49b, anti-mouse, Biotin, Clone REA541 Miltenyi Cat#130-108-199, RRID;AB_2658477 CD282 (TLR2), anti-human/mouse, APC, Clone REA109 Miltenyi Cat#130-120-052, RRID:AB_2751976 CD282 (TLR2), anti-human/mouse, functional grade, Clone REA109 Miltenyi Cat#130-098-855, RRID:AB_2661336 Dectin-1, anti-mouse, PE-Vio770, Clone REA154 Miltenyi Cat#130-102-989, RRID:AB_2651545 Dectin-1, anti-mouse, functional grade Invivogen Cat#mabg-mdect-2, RRID:AB_2753143 Dectin-2, anti-mouse, rabbit (host), Clone M-45 Santa-Cruz Cat#Sc-134906, RRID:AB_10610962 Mincle, anti-mouse, functional grade Invivogen Cat#mabg-mmcl-2, RRID: AB_3741008 Rat IgG2a Control Invivogen Cat#mabg2a-ctlrt, RRID:AB_11124905 Rat IgG2b Control Invivogen Cat#mabg2b-ctlrt, RRID: AB_3741009 Goat anti-rabbit IgG F(ab’), PE Santa-Cruz Cat#Sc-3843, RRID:AB_649073 Anti-rabbit IgG Fab2, Alexa Fluor 594 Cell Signaling Technology Cat#8889S, RRID:AB_2716249 anti-Candida, polyclonal, rabbit (host) Seiser et al.113 N/A CD90.2 (Thy1.2), anti-mouse, PerCP/ Cyanine5.5, Clone 30-H12 Biolegend Cat#105337, RRID:AB_2571944 CD90.2 (Thy1.2), anti-mouse, Brilliant Violet 785, Clone 30-H12 Biolegend Cat#105331, RRID:AB_2562900 CD90.2 (Thy1.2), anti-mouse, Super Bright 702, Clone 53–2.1 eBioscience; Fisher Scient. .. Cat#67-0902-80, RRID:AB_2717156 CD335 (NKp46/NCR1), anti-mouse, PerCP/Fire 780, Clone 29A1.4 Biolegend Cat#137651, RRID:AB_3083275 TruStain FcX PLUS (anti-mouse CD16/32), Clone S17011E Biolegend Cat#156603, RRID:AB_2783137 CD127 (IL-7Ralpha), anti-mouse, PE, Clone A7R34 Biolegend Cat#135009, RRID:AB_1937252 CD107a (LAMP-1), anti-mouse, PE, Clone 1D4B Biolegend Cat#121612, RRID:AB_10455386 NK1.1, anti-mouse, PE-eFluor 610, Clone PK136 eBioscience; Fisher Scient.



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    LPS from H. alvei strain BA2000346 activates both TLR4 and Dectin-2. ( A ) HEK-Blue TM human TLR (hTLR4) and ( B ) mouse Dectin-2 (mDectin-2) reporter cells were stimulated with LPS from E. coli O111:B4 or H. alvei BA2000346 for 24 h. Reporter activity was measured by absorbance at 655 nm. Data are shown as mean ± SD ( n = 6). Statistical significance was assessed using the Mann–Whitney test; ** p < 0.01. Comparisons were made against the negative control for hTLR4 and against E. coli O111:B4 for mDectin-2.

    Journal: Vaccines

    Article Title: Immunogenicity of an Oil-in-Water Emulsion Containing Hafnia Alvei -Derived Lipopolysaccharide, with TLR4 and Dectin-2 Agonist Activity In Vitro

    doi: 10.3390/vaccines14070557

    Figure Lengend Snippet: LPS from H. alvei strain BA2000346 activates both TLR4 and Dectin-2. ( A ) HEK-Blue TM human TLR (hTLR4) and ( B ) mouse Dectin-2 (mDectin-2) reporter cells were stimulated with LPS from E. coli O111:B4 or H. alvei BA2000346 for 24 h. Reporter activity was measured by absorbance at 655 nm. Data are shown as mean ± SD ( n = 6). Statistical significance was assessed using the Mann–Whitney test; ** p < 0.01. Comparisons were made against the negative control for hTLR4 and against E. coli O111:B4 for mDectin-2.

    Article Snippet: HEK-Blue TM human TLR4 (hTLR4) and mouse Dectin-2 (mDectin-2) reporter cells (InvivoGen) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, high glucose) supplemented with 10% FBS and 1% P/S.

    Techniques: Activity Assay, MANN-WHITNEY, Negative Control