Western Blot:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Immunohistochemistry:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Immunocytochemistry:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Binding Assay:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
In Vivo:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Injection:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Scaffolding:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Expressing:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Marker:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
MANN-WHITNEY:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
In Vitro:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Saline:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Incubation:Article Title: Anti-GluA3 autoantibodies define a new sub-population of frontotemporal lobar degeneration patients with distinct neuropathological features.
Article Snippet: The following primary antibodies were used: • mouse anti-GluA3 (MAB5416, Millipore; dilution: 1:1000 WB) • mouse anti-GluA2 (75-002, Neuromab; dilution: 1:2000 WB) • rabbit anti-GluA1 (13185S, Cell Signalling; dilution: 1:1,000 WB) • rabbit anti-GluN2A (M264, Sigma; dilution: 1:1000 WB) • rabbit anti-GluN2B (14544S, Cell Signalling; dilution: 1:1000 WB) • mouse anti-phospho tau (MN1020, Invitrogen; dilution: 1:1000 WB) • rabbit anti-phosphoTDP43 (22309-1-AP, Proteintech; dilution: 1:5000 WB) • rabbit anti-human-tau (A0024, Dako; dilution: 1:1000 WB) • rabbit anti-CD11b,c (bs-1014R, BIOSS; dilution: 1:1000 WB) • rat anti-CD68 (1957, Biorad; dilution: 1:400 IHC) • rabbit anti-Iba1 (019-19741, Fujifilm; dilution 1:700 IHC) • mouse anti-actin (A5441, Sigma; dilution: 1:5000 WB) • mouse anti-tubulin (T9026, Sigma-Aldrich; dilution: 1:5000 WB) • chicken anti-GFP (ABB16901, Millipore; dilution: 1:300 ICC) The following secondary antibodies were used: • goat anti-mouse-HRP (172–1011, Bio-Rad) • goat anti-rabbit-HRP (170–6515, Bio-Rad) • goat anti-rabbit-Alexa488 (A-11034, Invitrogen) • goat anti-rat-Alexa488 (A11006, Invitrogen) • goat anti-rabbit-Alexa555 (A21428, Invitrogen) • goat anti-human-Alexa 488 IgG (A11013, Invitrogen; dilution 1:1000 IHC) • goat anti-human-Alexa555 (A-21433, Invitrogen; dilution 1:1000 IHC)
Article Title: Complex inhibitory microcircuitry regulates retinal signaling near visual threshold
Article Snippet: Grids were washed with distilled H 2 O followed by a Tris-buffered saline wash (TBS: 0.05 M Tris buffer, 0.7% NaCl, pH 7.6), incubated in 5% BSA in TBS for 30 min, and then incubated overnight at RT in one of several antibody solutions: 1 ) mouse anti-GluA3 [Millipore; monoclonal, raised against residues 245–451 (NH 2 terminus); dilution 1:50]; 2 ) a cocktail of rabbit anti-GABA C R [a generous gift from Dr. Heinz Wässle; polyclonal, raised against residues 16–171 (NH 2 terminus) of the ρ-subunit; 1:100] and goat anti-GABA A R subunit (Santa Cruz Biotechnology; polyclonals, raised against the cytoplasmic domains of α1 and α3, 1:100; β2 and β3, 1:100; γ3, 1:50); 3 ) rabbit anti-BK α subunit [Sigma; polyclonal, raised against residues 1,184-1,200 (NH 2 -terminus); 1:100].
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anticaspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
Article Snippet: The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich).
Article Title: Immunohistochemical localization of ionotropic glutamate receptors in the rat red nucleus
Article Snippet: The sections were incubated in rabbit anti-GluA1 (1:250, AB1504, Chemicon, USA), mouse anti-GluA2 (1:500, MAB 397, Chemicon, USA), mouse anti-GluA3 (1:250, MAB5416, Chemicon, USA), rabbit anti-GluA4 (1:250, AB1508, Chemicon, USA), goat anti-GluK1 (1:250, sc-7617, Santa Cruz, USA) rabbit anti-GluK5 (1:2000, Jennes and Eyigor, R52-4) [ 30 ], and guinea pig anti-GluK2/3 (1:500, Jennes and Eyigor) [ 31 ], mouse anti-GluN1 (1:500, 556308, BD Pharmingen, USA) or mouse anti-GluN2A (1:3000, MAB5216, Millipore, USA) for 48 hours at room temperature.
Article Title: Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
Article Snippet: The primary antibodies used were: mouse anti-tubulin (1:30,000, #T9026, Sigma), rabbit anti-GAPDH (1:5,000, #sc-25778, Santa Cruz), rabbit anti-GluN2A (1:1,000, #M264, Sigma), rabbit anti-GluN2B (1:1,000, #718600, Invitrogen), mouse anti-GluN2D (1:1,000, #MAB5578, Millipore), rabbit anti-GluA1 (1:1,000, #13185, Cell Signaling), mouse anti-GluA2 (1:1,000, #75–002, Neuromab), mouse anti-GluA3 (1:1,000, #MAB5416, Millipore), polyclonal anti-Rph3A (1:2,000, Protein Tech, #11396-1-AP), mouse anti-PSD-95 (1:1,000, #K28/43, Neuromab), rabbit anti-tyrosine hydroxylase (1:10,000, #AB152, Millipore), rabbit anti-phospho-extracellular signal-regulated kinase (ERK) 44/42 (1:1,000, Cell Signaling, #9101), rabbit anti-ERK 44/42 (1:1,000, #9102, Cell Signaling), rabbit anti-phospho-cAMP responsive element binding protein (CREB) (1:1,000, #9198, Cell Signaling), and rabbit anti-CREB (1:1,000, #9197, Cell Signaling).
Article Title: Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling
Article Snippet: The following primary antibodies were used: mouse anti-α-tubulin (Sigma-Aldrich #T6074, 1:10000), mouse anti-PSD95 (Thermo Fisher Scientific #MA1-045; 1:1000), rabbit anti-caspase-2 (Abcam #ab179519, 1:2000), mouse anti-β-actin (Sigma-Aldrich #A5441, 1:10000), mouse anti-synaptophysin (Abcam #ab8049, 1:1000), rabbit anti-GluA1 (Millipore #AB1504, 1:1000), mouse anti-GluA2 (Millipore #MABN71, 1:1000), mouse anti-GluA3 (Millipore #MAB5416, 1:1000), mouse anti-GluN1 (Millipore #MAB363, 1:1000), mouse anti-PDK1 (Santa Cruz Biotechnology #sc-293160, 1:1000), mouse anti-PDK2 (Santa Cruz Biotechnology #sc-517284, 1:1000), mouse anti-p110 (Santa Cruz Biotechnology #sc-8010, 1:500), mouse anti-Rictor (Santa Cruz Biotechnology #sc271081, 1:500), rabbit anti-S473-Akt (Cell Signaling Technology #2920, 1:1000), rabbit anti-T308-Akt (Cell Signaling Technology #9275, 1:1000), mouse anti-pan Akt (Cell Signaling Technology #9271, 1:1000), rabbit anti-GSK3β (Cell Signaling Technology #9315, 1:1000), rabbit anti-Ser9-GSK3β (Cell Signaling Technology #9331, 1:1000), rabbit anti-mTOR (Cell Signaling Technology #2972, 1:1000), rabbit anti-Histone H3 (Cell Signaling Technology #4499, 1:1000), and rabbit anti-pS6K (Cell Signaling Technology #9205, 1:1000).
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