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mouse anti ratcd2  (Bio-Rad)


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    Structured Review

    Bio-Rad mouse anti ratcd2
    Mouse Anti Ratcd2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+ratcd2/Mouse+anti+Rat+CD2/pm40765444-66-128-132
    Average 93 stars, based on 196 article reviews
    mouse anti ratcd2 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Control:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Expressing:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Knockdown:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Blocking Assay:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Virus:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Recombinant:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Sequencing:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Software:

    Article Title: Glia multitask to compensate for neighboring glial cell dysfunction
    Article Snippet: Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.Subsequently, tissue was stained over 2 nights at 4℃ with antibodies in 0.3% PTX.. The following primary antibodies were used: chicken anti-GFP (1:1000; Antibodies Inc), rat anti- mCherry (1:1000, Invitrogen); rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100; Developmental Studies Hybridoma Bank [DSHB], 7E8A10), mouse anti-Elav (1:100, DSHB, 9F8A9), Anti-β-Galactosidase (1:1000; Promega, Z3781), mouse anti-Repo (1:5; DSHB, 8D12), mouse anti-Prospero (1:100; DSHB, MR1A), rabbit anti-DCP1 (1:100; Cell Signaling, 9578S), rabbit anti-corazonin (1:500, Freeman Lab), mouse anti-nc82 (1:20; DSHB, nc82), mouse anti- ratCD2 (1:200; BioRad, MCA154GA), rabbit anti-p35 (1:1000, Fisher Scientific, NB10056153T).. Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and rat [712-475-153]), Dylight488 (chicken [103-005-155]), Cy3 (rabbit [711-165-152], mouse [715-165-151], and rat [712-175-153]), or Cy5 (rabbit [711-175-152), mouse [715-175-151], and rat [712-175-150]) from Jackson ImmunoResearch.Primary antibodies were detected with the appropriate goat or donkey secondary antibodies conjugated to Dylight405 (rabbit [711-475-152], mouse [715-475-151], and..

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.

    Article Title: Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing
    Article Snippet: All wash steps were performed at room temperature.All wash steps were performed at room temperature.. Primary antibodies used in this study include rat anti-DNcad (DN-Ex #8; 1:40; DSHB), mouse anti-Prospero (1:200; DSHB), mouse anti-Cut (2B10; 1:50; DSHB), mouse anti-β-gal (1:500; Promega), chicken anti-GFP (1:1000; Aves Labs), rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34; 1:200; AbD Serotec), rat anti-C15 (1:200; gift from Dr. Gerard Campbell) ( Campbell, 2005 ), and guinea pig anti-knot (1:200; gift from Dr. Adrian Moore) ( Jinushi-Nakao et al., 2007 ).. Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.Secondary antibodies were raised in goat or donkey against rabbit, mouse, rat, and chicken antisera (Jackson Immunoresearch), conjugated to Alexa 405, 488, FITC, Cy3, Cy5, or Alexa 647.



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    KEY RESOURCES TABLE

    Journal: Current biology : CB

    Article Title: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting

    doi: 10.1016/j.cub.2020.01.049

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rat anti-ratCD2 , AbD Serotec , OX-34.

    Techniques: Virus, Recombinant, Sequencing, Software