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mouse anti glua1  (NeuroMab)


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    NeuroMab mouse anti glua1
    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, <t>GluA1</t> or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.
    Mouse Anti Glua1, supplied by NeuroMab, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+glua1/Anti-GluA1-GluR1+Glutamate+Receptor+Antibody/pmc12663429-490-128-131
    Average 95 stars, based on 55 article reviews
    mouse anti glua1 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder"

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder

    Journal: Nature Communications

    doi: 10.1038/s41467-025-66504-2

    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, GluA1 or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.
    Figure Legend Snippet: a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, GluA1 or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.

    Techniques Used: Immunoprecipitation, Western Blot, Control, Two Tailed Test

    a Number of phosphoproteins reaching a significance threshold of nominal P < 0.05. Fractions of upregulated and downregulated DAPPs are highlighted in red and blue, respectively. b Volcano plots of 12wk synapse phosphoproteomics data for Akap11 +/- and Akap11 -/- vs. WT controls. Phosphoproteomic measurements are normalized to MS-proteomics measurements from the same samples. Known PKA Cα substrates from PhosphoSitePlus are labeled green. AKAP11 phosphopeptides are omitted from Akap11 -/- plots, for clarity. a,b A moderated two-sample t-test was applied to the datasets to compare WT, Akap11 +/- and Akap11 -/- sample groups. c Motif analysis of peptides flanking the phosphorylation site of P < 0.05 phosphosites in A. n(fg) are the number of foreground peptides, and n(bg) are the number of background peptides. Red lines indicate FDR significance, calculated by log odds enrichment. d PTM-SEA of synapse phosphoproteomics data, using known kinase substrates from PhosphoSitePlus. Black borders indicate FDR significance. e ELISA-based PKA activity measurements comparing total lysate and synapse fractions of cortex (left) and striatum-enriched tissue (right). One Unit (U) is defined by the manufacturer as the quantity of PKA that catalyzes the transfer of 1.0 pmol phosphate from ATP to substrate. Two-way ANOVA with Tukey’s post hoc test. f Immunoblotting and quantification of AKAP11, PKA subunits, p-GluA1 S845 and GluA1 in 12wk cortical total lysate. One-way ANOVA with Tukey’s post hoc test. e,f Data are represented as mean ± SEM. See Supplementary Data for detailed statistical information.
    Figure Legend Snippet: a Number of phosphoproteins reaching a significance threshold of nominal P < 0.05. Fractions of upregulated and downregulated DAPPs are highlighted in red and blue, respectively. b Volcano plots of 12wk synapse phosphoproteomics data for Akap11 +/- and Akap11 -/- vs. WT controls. Phosphoproteomic measurements are normalized to MS-proteomics measurements from the same samples. Known PKA Cα substrates from PhosphoSitePlus are labeled green. AKAP11 phosphopeptides are omitted from Akap11 -/- plots, for clarity. a,b A moderated two-sample t-test was applied to the datasets to compare WT, Akap11 +/- and Akap11 -/- sample groups. c Motif analysis of peptides flanking the phosphorylation site of P < 0.05 phosphosites in A. n(fg) are the number of foreground peptides, and n(bg) are the number of background peptides. Red lines indicate FDR significance, calculated by log odds enrichment. d PTM-SEA of synapse phosphoproteomics data, using known kinase substrates from PhosphoSitePlus. Black borders indicate FDR significance. e ELISA-based PKA activity measurements comparing total lysate and synapse fractions of cortex (left) and striatum-enriched tissue (right). One Unit (U) is defined by the manufacturer as the quantity of PKA that catalyzes the transfer of 1.0 pmol phosphate from ATP to substrate. Two-way ANOVA with Tukey’s post hoc test. f Immunoblotting and quantification of AKAP11, PKA subunits, p-GluA1 S845 and GluA1 in 12wk cortical total lysate. One-way ANOVA with Tukey’s post hoc test. e,f Data are represented as mean ± SEM. See Supplementary Data for detailed statistical information.

    Techniques Used: Phospho-proteomics, Labeling, Enzyme-linked Immunosorbent Assay, Activity Assay, Western Blot

    Related Articles

    Western Blot:

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder
    Article Snippet: When the IP and immunoblot antibodies were from the same species , we used secondaries that are selective towards non-denatured IgG (1:1000, TrueBlot HRP, Rockland, 18-8816-33 and 18-8817-30). .. For immunoblot, the following antibodies were used: 1:1000 Rabbit anti-AKAP11 (Custom R171), 1:1000 Rabbit anti-AKAP11 (Custom R173), 1:1000 Mouse anti-PKA R1α (Thermo Fisher Scientific MA5-24981), 1:1000 Rabbit anti-PKA R1α (CST 5675), 1:1000 Rabbit anti-PKA Cα (CST 4782), 1:1000 Rabbit anti-Iqgap1 (Abcam ab86064), 1:1000 Rabbit anti-Dyrk1a (CST 2771), 1:1000 Rabbit anti-GSK3α (CST 4818), 1:1000 Rabbit anti-GSK3β (CST 9315), 1:1000 Mouse anti-P62 (Abcam ab56416), 1:1000 Rabbit anti-LC3A/B (CST 12741), 1:1000 Rabbit anti-VAPA (Proteintech 15275-1-AP), 1:1000 Rabbit anti-β Catenin (CST 8480), 1:1000 Mouse anti-Protein Phosphatase 1 (Santa Cruz SC-7482), 1:1000 Rabbit anti-Sik2 (CST 6919), 1:1000 Rabbit anti-p-GSK3α S / anti-p-GSK3β S (CST 8566), 1:1000 Rabbit anti-p-GSK3α / anti-p-GSK3β (CST 5676), 1:1000 Rabbit anti-p-GSK3β S (CST 9336), 1:1000 Rabbit anti-p-GluA1 S845 (CST 8084), 1:1000 Rabbit anti-GluA1 (CST 13185), 1:1000 Mouse anti-GluA1 (NeuroMab 75-327), 1:1000 Mouse anti-PSD95 (BioLegend 810301), 1:1000 Rabbit anti-Homer1 (Synaptic Systems 160003), 1:1000 Guinea Pig anti-Synaptophysin 1(Synaptic Systems 101004). .. 1:1000 Mouse anti-PSD95 (clone K28/43, Antibodies Inc 75-028).

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder
    Article Snippet: When the IP and immunoblot antibodies were from the same species (Fig. ), we used secondaries that are selective toward non-denatured IgG (1:1000, TrueBlot HRP, Rockland, 18-8816-33 and 18-8817-30). .. For immunoblot, the following antibodies were used: 1:1000 Rabbit anti-AKAP11 (Custom R171), 1:1000 Rabbit anti-AKAP11 (Custom R173), 1:1000 Mouse anti-PKA R1α (Thermo Fisher Scientific MA5-24981), 1:1000 Rabbit anti-PKA R1α (CST 5675), 1:1000 Rabbit anti-PKA Cα (CST 4782), 1:1000 Rabbit anti-Iqgap1 (Abcam ab86064), 1:1000 Rabbit anti-Dyrk1a (CST 2771), 1:1000 Rabbit anti-GSK3α (CST 4818), 1:1000 Rabbit anti-GSK3β (CST 9315), 1:1000 Mouse anti-P62 (Abcam ab56416), 1:1000 Rabbit anti-LC3A/B (CST 12741), 1:1000 Rabbit anti-VAPA (Proteintech 15275-1-AP), 1:1000 Rabbit anti-β Catenin (CST 8480), 1:1000 Mouse anti-Protein Phosphatase 1 (Santa Cruz SC-7482), 1:1000 Rabbit anti-Sik2 (CST 6919), 1:1000 Rabbit anti-p-GSK3α S21 / anti-p-GSK3β S9 (CST 8566), 1:1000 Rabbit anti-p-GSK3α / anti-p-GSK3β (CST 5676), 1:1000 Rabbit anti- p-GSK3β S9 (CST 9336), 1:1000 Rabbit anti- p-GluA1 S845 (CST 8084), 1:1000 Rabbit anti- GluA1 (CST 13185), 1:1000 Mouse anti- GluA1 (NeuroMab 75-327), 1:1000 Mouse anti-PSD95 (BioLegend 810301), 1:1000 Rabbit anti-Homer1 (Synaptic Systems 160003), 1:1000 Guinea Pig anti-Synaptophysin 1(Synaptic Systems 101004). .. 1:1000 Mouse anti-PSD95 (clone K28/43, Antibodies Inc 75-028).

    other:


    Immunoprecipitation:

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder
    Article Snippet: .. For coIP-immunoblot, the following antibodies were used during SDS-page (after immunoprecipitation): 1:1000 Mouse anti-PKA R1α (BD 610609), 1:1000 Mouse anti-PKA Cα (BD 610980), 1:1000 Mouse anti-PKA R2β (BD 610625), 1:1000 Mouse anti-GluA1 (NeuroMab 75-327), 1:1000 Rabbit anti-Vinculin (CST 4650), 1:1000 Rabbit anti-VAPA (Proteintech 15275-1-AP), 1:1000 Rabbit anti-VAPB (Proteintech 14477-1-AP), 1:1000 Rabbit anti-GSK3α (CST 4337), 1:1000 Rabbit anti-GSK3β (CST 9315), Mouse anti-GSK3α/β (Thermo Fisher Scientific 44-610), 1:1000 Rabbit anti-Dyrk1a (CST 2771), 1:1000 Rabbit anti-P62 (CST 5114). .. For coIP-immunoblot, we used horseradish peroxidase (HRP)-conjugated secondaries recognizing the host species of the primary antibody (Thermo Fisher Scientific 31460 and 31430 for rabbit and mouse respectively).

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder
    Article Snippet: .. For coIP-immunoblot, the following antibodies were used during SDS-page (after immunoprecipitation): 1:1000 Mouse anti-PKA R1α (BD 610609), 1:1000 Mouse anti-PKA Cα (BD 610980), 1:1000 Mouse anti-PKA R2β (BD 610625), 1:1000 Mouse anti-GluA1 (NeuroMab 75-327), 1:1000 Rabbit anti-Vinculin (CST 4650), 1:1000 Rabbit anti-VAPA (Proteintech 15275-1-AP), 1:1000 Rabbit anti-VAPB (Proteintech 14477-1-AP), 1:1000 Rabbit anti-GSK3α (CST 4337), 1:1000 Rabbit anti-GSK3β (CST 9315), Mouse anti-GSK3α/β (Thermo Fisher Scientific 44-610) ,1:1000 Rabbit anti-Dyrk1a (CST 2771), 1:1000 Rabbit anti-P62 (CST 5114). .. For coIP-immunoblot, we used horseradish peroxidase (HRP)-conjugated secondaries recognizing the host species of the primary antibody (Thermo Fisher Scientific 31460 and 31430 for rabbit and mouse respectively).

    Blocking Assay:

    Article Title: High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
    Article Snippet: .. After blocking, membranes were incubated for 1 h at RT, or overnight at 4°C, with the primary antibody diluted in TBST solution containing 0,5% dry milk: rabbit anti-NLGN1 (Synaptic Systems, #129-013, 1:1000); rabbit anti-NLGN2 (Synaptic Systems, #129-202, 1:1000) ; rabbit anti-NLGN3 (Synaptic Systems, #129-113, 1:1000); rabbit anti-MDGA1 (Synaptic Systems, #421-002, 1:500); mouse anti-PSD-95 (Invitrogen, clone 7E3-1B8 #MA1-046, 1:1000); mouse anti-gephyrin (Synaptic Systems, clone 3B11 #147-111, 1:2000); mouse anti-GluA1 (Neuromab, #75-327, 1:1000); mouse anti-GluA2 (Sigma-Aldrich, #MAB397, 1:1000) ; mouse anti-GluN1 (Sigma-Aldrich, clone 54.1 #MAB363, 1:1000); mouse anti-βactin (Sigma-Aldrich, #A5316, 1:5000); mouse anti-HA (BioLegend, #901513, 1:1000); mouse anti-GFP (Roche, #11814460001, 1:1000); rabbit anti-PSD-95 (Cell Signaling, clone D27E11 #3450, 1:1000). .. After three washes in TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated donkey anti-mouse or anti-rabbit secondary antibodies (Jackson Immunoresearch, #715-035-150 and #711- 035-152, 1:5000) for 1 hr at RT.

    Incubation:

    Article Title: High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
    Article Snippet: .. After blocking, membranes were incubated for 1 h at RT, or overnight at 4°C, with the primary antibody diluted in TBST solution containing 0,5% dry milk: rabbit anti-NLGN1 (Synaptic Systems, #129-013, 1:1000); rabbit anti-NLGN2 (Synaptic Systems, #129-202, 1:1000) ; rabbit anti-NLGN3 (Synaptic Systems, #129-113, 1:1000); rabbit anti-MDGA1 (Synaptic Systems, #421-002, 1:500); mouse anti-PSD-95 (Invitrogen, clone 7E3-1B8 #MA1-046, 1:1000); mouse anti-gephyrin (Synaptic Systems, clone 3B11 #147-111, 1:2000); mouse anti-GluA1 (Neuromab, #75-327, 1:1000); mouse anti-GluA2 (Sigma-Aldrich, #MAB397, 1:1000) ; mouse anti-GluN1 (Sigma-Aldrich, clone 54.1 #MAB363, 1:1000); mouse anti-βactin (Sigma-Aldrich, #A5316, 1:5000); mouse anti-HA (BioLegend, #901513, 1:1000); mouse anti-GFP (Roche, #11814460001, 1:1000); rabbit anti-PSD-95 (Cell Signaling, clone D27E11 #3450, 1:1000). .. After three washes in TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated donkey anti-mouse or anti-rabbit secondary antibodies (Jackson Immunoresearch, #715-035-150 and #711- 035-152, 1:5000) for 1 hr at RT.



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    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, <t>GluA1</t> or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.
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    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, <t>GluA1</t> or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.
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    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, <t>GluA1</t> or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.
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    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, <t>GluA1</t> or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.
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    Image Search Results


    a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, GluA1 or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.

    Journal: Nature Communications

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder

    doi: 10.1038/s41467-025-66504-2

    Figure Lengend Snippet: a Schematic of AKAP11 immunoprecipitation (IP) and its most notable, known interactors. b Immunoblot of AKAP11 in IPs of anti-AKAP11 and control IgG in 12-week-old (12wk) WT or Akap11 -/- cortical tissue. This experiment was repeated in >5 independent experiments, with similar results. c Volcano plot comparing Log Fold-Change (Log FC) against P value for AKAP11 IP in WT vs Akap11 -/- , displaying all proteins detected by coIP-MS. A moderated two-sample t-test was applied to the sample group datasets. Proteins that were enriched (red) by anti-AKAP11 IP from WT versus Akap11 -/- cortical extracts. Enriched proteins, defined by having a nominal P value < 0.05 and Log FC > 0, are colored red. Downregulated proteins are mostly antibody-related artifacts and can be found in Supplementary Data . d Venn diagram comparing previously published AKAP11 interactions with the brain interactome in this study. Full results are displayed in Supplementary Data . e Immunoblot of AKAP11, PKA subunits and control proteins, in IPs of AKAP11, GluA1 or IgG control in WT or Akap11 -/- cortical lysate. This experiment was repeated in >3 independent experiments, with similar results. f Immunoblot of AKAP11 in IPs of PKA subunits, in WT cortical lysates. This experiment was repeated in >2 independent experiments, with similar results. g–j Immunoblot with indicated antibodies, in IPs of anti-GSK3α, GSK3β, VAPA, VAPB, AKAP11, P62 or DYRK1a in WT or Akap11 -/- cortical lysate. These experiments were repeated in at least 2 independent experiments, with similar results. k Gene set enrichment analysis of all proteins displayed in 1c, showing selected gene ontologies with a positive normalized enrichment score (NES) and adjusted P -value (FDR) < 0.01. Redundant (similar) pathways were removed for clarity. The top most enriched proteins within each ontology are listed within the histograms. GSEA uses a Kolmogorov-Smirnov test, two tailed, with Benjamini-Hochberg (B-H) False Discovery Rate (FDR) correction applied.

    Article Snippet: For immunoblot, the following antibodies were used: 1:1000 Rabbit anti-AKAP11 (Custom R171), 1:1000 Rabbit anti-AKAP11 (Custom R173), 1:1000 Mouse anti-PKA R1α (Thermo Fisher Scientific MA5-24981), 1:1000 Rabbit anti-PKA R1α (CST 5675), 1:1000 Rabbit anti-PKA Cα (CST 4782), 1:1000 Rabbit anti-Iqgap1 (Abcam ab86064), 1:1000 Rabbit anti-Dyrk1a (CST 2771), 1:1000 Rabbit anti-GSK3α (CST 4818), 1:1000 Rabbit anti-GSK3β (CST 9315), 1:1000 Mouse anti-P62 (Abcam ab56416), 1:1000 Rabbit anti-LC3A/B (CST 12741), 1:1000 Rabbit anti-VAPA (Proteintech 15275-1-AP), 1:1000 Rabbit anti-β Catenin (CST 8480), 1:1000 Mouse anti-Protein Phosphatase 1 (Santa Cruz SC-7482), 1:1000 Rabbit anti-Sik2 (CST 6919), 1:1000 Rabbit anti-p-GSK3α S21 / anti-p-GSK3β S9 (CST 8566), 1:1000 Rabbit anti-p-GSK3α / anti-p-GSK3β (CST 5676), 1:1000 Rabbit anti- p-GSK3β S9 (CST 9336), 1:1000 Rabbit anti- p-GluA1 S845 (CST 8084), 1:1000 Rabbit anti- GluA1 (CST 13185), 1:1000 Mouse anti- GluA1 (NeuroMab 75-327), 1:1000 Mouse anti-PSD95 (BioLegend 810301), 1:1000 Rabbit anti-Homer1 (Synaptic Systems 160003), 1:1000 Guinea Pig anti-Synaptophysin 1(Synaptic Systems 101004).

    Techniques: Immunoprecipitation, Western Blot, Control, Two Tailed Test

    a Number of phosphoproteins reaching a significance threshold of nominal P < 0.05. Fractions of upregulated and downregulated DAPPs are highlighted in red and blue, respectively. b Volcano plots of 12wk synapse phosphoproteomics data for Akap11 +/- and Akap11 -/- vs. WT controls. Phosphoproteomic measurements are normalized to MS-proteomics measurements from the same samples. Known PKA Cα substrates from PhosphoSitePlus are labeled green. AKAP11 phosphopeptides are omitted from Akap11 -/- plots, for clarity. a,b A moderated two-sample t-test was applied to the datasets to compare WT, Akap11 +/- and Akap11 -/- sample groups. c Motif analysis of peptides flanking the phosphorylation site of P < 0.05 phosphosites in A. n(fg) are the number of foreground peptides, and n(bg) are the number of background peptides. Red lines indicate FDR significance, calculated by log odds enrichment. d PTM-SEA of synapse phosphoproteomics data, using known kinase substrates from PhosphoSitePlus. Black borders indicate FDR significance. e ELISA-based PKA activity measurements comparing total lysate and synapse fractions of cortex (left) and striatum-enriched tissue (right). One Unit (U) is defined by the manufacturer as the quantity of PKA that catalyzes the transfer of 1.0 pmol phosphate from ATP to substrate. Two-way ANOVA with Tukey’s post hoc test. f Immunoblotting and quantification of AKAP11, PKA subunits, p-GluA1 S845 and GluA1 in 12wk cortical total lysate. One-way ANOVA with Tukey’s post hoc test. e,f Data are represented as mean ± SEM. See Supplementary Data for detailed statistical information.

    Journal: Nature Communications

    Article Title: Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder

    doi: 10.1038/s41467-025-66504-2

    Figure Lengend Snippet: a Number of phosphoproteins reaching a significance threshold of nominal P < 0.05. Fractions of upregulated and downregulated DAPPs are highlighted in red and blue, respectively. b Volcano plots of 12wk synapse phosphoproteomics data for Akap11 +/- and Akap11 -/- vs. WT controls. Phosphoproteomic measurements are normalized to MS-proteomics measurements from the same samples. Known PKA Cα substrates from PhosphoSitePlus are labeled green. AKAP11 phosphopeptides are omitted from Akap11 -/- plots, for clarity. a,b A moderated two-sample t-test was applied to the datasets to compare WT, Akap11 +/- and Akap11 -/- sample groups. c Motif analysis of peptides flanking the phosphorylation site of P < 0.05 phosphosites in A. n(fg) are the number of foreground peptides, and n(bg) are the number of background peptides. Red lines indicate FDR significance, calculated by log odds enrichment. d PTM-SEA of synapse phosphoproteomics data, using known kinase substrates from PhosphoSitePlus. Black borders indicate FDR significance. e ELISA-based PKA activity measurements comparing total lysate and synapse fractions of cortex (left) and striatum-enriched tissue (right). One Unit (U) is defined by the manufacturer as the quantity of PKA that catalyzes the transfer of 1.0 pmol phosphate from ATP to substrate. Two-way ANOVA with Tukey’s post hoc test. f Immunoblotting and quantification of AKAP11, PKA subunits, p-GluA1 S845 and GluA1 in 12wk cortical total lysate. One-way ANOVA with Tukey’s post hoc test. e,f Data are represented as mean ± SEM. See Supplementary Data for detailed statistical information.

    Article Snippet: For immunoblot, the following antibodies were used: 1:1000 Rabbit anti-AKAP11 (Custom R171), 1:1000 Rabbit anti-AKAP11 (Custom R173), 1:1000 Mouse anti-PKA R1α (Thermo Fisher Scientific MA5-24981), 1:1000 Rabbit anti-PKA R1α (CST 5675), 1:1000 Rabbit anti-PKA Cα (CST 4782), 1:1000 Rabbit anti-Iqgap1 (Abcam ab86064), 1:1000 Rabbit anti-Dyrk1a (CST 2771), 1:1000 Rabbit anti-GSK3α (CST 4818), 1:1000 Rabbit anti-GSK3β (CST 9315), 1:1000 Mouse anti-P62 (Abcam ab56416), 1:1000 Rabbit anti-LC3A/B (CST 12741), 1:1000 Rabbit anti-VAPA (Proteintech 15275-1-AP), 1:1000 Rabbit anti-β Catenin (CST 8480), 1:1000 Mouse anti-Protein Phosphatase 1 (Santa Cruz SC-7482), 1:1000 Rabbit anti-Sik2 (CST 6919), 1:1000 Rabbit anti-p-GSK3α S21 / anti-p-GSK3β S9 (CST 8566), 1:1000 Rabbit anti-p-GSK3α / anti-p-GSK3β (CST 5676), 1:1000 Rabbit anti- p-GSK3β S9 (CST 9336), 1:1000 Rabbit anti- p-GluA1 S845 (CST 8084), 1:1000 Rabbit anti- GluA1 (CST 13185), 1:1000 Mouse anti- GluA1 (NeuroMab 75-327), 1:1000 Mouse anti-PSD95 (BioLegend 810301), 1:1000 Rabbit anti-Homer1 (Synaptic Systems 160003), 1:1000 Guinea Pig anti-Synaptophysin 1(Synaptic Systems 101004).

    Techniques: Phospho-proteomics, Labeling, Enzyme-linked Immunosorbent Assay, Activity Assay, Western Blot