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mouse antihuman cd45 alexa fluor 647  (Bio-Rad)


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    Structured Review

    Bio-Rad mouse antihuman cd45 alexa fluor 647
    Mouse Antihuman Cd45 Alexa Fluor 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd45/Mouse+anti+Human+CD45/pmc13691775-62-47-55
    Average 93 stars, based on 141 article reviews
    mouse antihuman cd45 alexa fluor 647 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Trajectory of inflammatory and microglial activation markers in the postnatal rabbit brain following intrauterine endotoxin exposure
    Article Snippet: 30 μm sections were cut with a cryostat and mounted onto poly-L-lysine coated slides (Sigma Aldrich, MO, USA). .. The sections were blocked with 5% donkey serum, incubated with goat anti-IBA1 (1:250, Abcam, MA, USA), rat anti-CD11b (1:100, Genetex, CA, USA) and mouse anti-CD45 (1:100, Bio-Rad, CA, USA), or goat anti-IBA1 (1:250, Abcam, MA, USA) and rabbit anti-iNOS (1:50, Thermo Fisher Scientific, MA, USA), followed by fluorescent secondary antibodies (1:250, Thermo Fisher Scientific, MA, USA) for 2h. .. For TSPO and Lectin co-staining, sections were blocked with 5% donkey serum, and incubated with goat anti-TSPO (1:500, GeneTex, CA, USA) overnight at 4°C.

    Article Title: Perivascular delivery of resolvin D1 inhibits neointimal hyperplasia in a rabbit vein graft model
    Article Snippet: Sections were then permeabilized with 0.2% Tween 20 (Sigma-Aldrich) and blocked in 5% goat serum (Sigma-Aldrich). .. Blocking of endogenous avidin-biotin was performed with a commercial kit (Vector, Burlingame, Calif) before incubation with one of the following primary antibodies: mouse-anti-CD45 (1:50; AbD Serotec, Hercules, Calif), mouse-anti-RAM11 (1:50 for frozen samples and 1:200 for paraffin-embedded samples; Dako, Santa Clara, Calif), mouse-anti-Ki67 (1:50; Dako), or mouse-anti-a-smooth muscle actin (1:200; Sigma-Aldrich). .. This was followed by incubation with a biotin-goat-anti-mouse secondary (1:200; BioLegends, San Diego, Calif), then a fluorescein isothiocyanate-conjugated streptavidin (1:200; Dako).

    Article Title: A novel tropoelastin-based resorbable surgical mesh for pelvic organ prolapse repair
    Article Snippet: .. Immunohistochemistry staining was performed on FFPE sections following antigen retrieval using 0.1 M citrate buffer, blocking endogenous peroxidase with 3% H 2 O 2 , incubation with protein block (Dako) for 30 min at room temperature, using mouse anti-CD45 (0.5 μg/mL, BioRad) and mouse anti-CD206 (0.5 μg/mL, Dendritics) primary antibodies for 1 h at 37C. ..

    Blocking Assay:

    Article Title: Perivascular delivery of resolvin D1 inhibits neointimal hyperplasia in a rabbit vein graft model
    Article Snippet: Sections were then permeabilized with 0.2% Tween 20 (Sigma-Aldrich) and blocked in 5% goat serum (Sigma-Aldrich). .. Blocking of endogenous avidin-biotin was performed with a commercial kit (Vector, Burlingame, Calif) before incubation with one of the following primary antibodies: mouse-anti-CD45 (1:50; AbD Serotec, Hercules, Calif), mouse-anti-RAM11 (1:50 for frozen samples and 1:200 for paraffin-embedded samples; Dako, Santa Clara, Calif), mouse-anti-Ki67 (1:50; Dako), or mouse-anti-a-smooth muscle actin (1:200; Sigma-Aldrich). .. This was followed by incubation with a biotin-goat-anti-mouse secondary (1:200; BioLegends, San Diego, Calif), then a fluorescein isothiocyanate-conjugated streptavidin (1:200; Dako).

    Article Title: A novel tropoelastin-based resorbable surgical mesh for pelvic organ prolapse repair
    Article Snippet: .. Immunohistochemistry staining was performed on FFPE sections following antigen retrieval using 0.1 M citrate buffer, blocking endogenous peroxidase with 3% H 2 O 2 , incubation with protein block (Dako) for 30 min at room temperature, using mouse anti-CD45 (0.5 μg/mL, BioRad) and mouse anti-CD206 (0.5 μg/mL, Dendritics) primary antibodies for 1 h at 37C. ..

    Article Title: Discovery of a CCR2-targeting pepducin therapy for chronic pain.
    Article Snippet: .. Sections were labeled with mouse antipERK (1:100, Cell Signaling #9101), mouse anti-CD45 (1:500, BioRad #MCA43R), mouse anti-CD68 (1:500, Bio-Rad #MCA341R), mouse anti-CD11b (1:500, Millipore, #CBL1512), and mouse anti-NeuN (1:500, Millipore #MAB377), rabbit anti-CCL2 (1:100, PeproTech #500-P76), rabbit anti-connexin 43 (1:500, Sigma #6219), rabbit antiGFAP (1:500, Sigma, #G3893), rabbit anti-Iba1 (1:500, Wako #019–19741), rabbit anti-pP38 (1:100, Cell Signaling, #4511), rabbit anti-Ki67 (1:100, Abcam #AB16667), and mouse anti-pJNK (1:100, Cell Signaling #9251), chicken anti-CCR2 (1:200, Aves Labs, custom) and chicken anti-CGRP (1:500, Millipore #AB5705), guinea-pig anti-substance P (1:500, Neuromics #GP14110), hamster anti-CD3 (1:500, Santa Cruz, #sc-1174) in blocking buffer for three days at 37◦C for spinal cord, 24 hour at R.T. for DRG. .. They were further incubated with fluorophoreconjugated secondary antibodies (1:500, AlexaFluor 488, 568, 647, Invitrogen) and spinal cord sections were mounted on SuperFrost Plus slides.

    Avidin-Biotin Assay:

    Article Title: Perivascular delivery of resolvin D1 inhibits neointimal hyperplasia in a rabbit vein graft model
    Article Snippet: Sections were then permeabilized with 0.2% Tween 20 (Sigma-Aldrich) and blocked in 5% goat serum (Sigma-Aldrich). .. Blocking of endogenous avidin-biotin was performed with a commercial kit (Vector, Burlingame, Calif) before incubation with one of the following primary antibodies: mouse-anti-CD45 (1:50; AbD Serotec, Hercules, Calif), mouse-anti-RAM11 (1:50 for frozen samples and 1:200 for paraffin-embedded samples; Dako, Santa Clara, Calif), mouse-anti-Ki67 (1:50; Dako), or mouse-anti-a-smooth muscle actin (1:200; Sigma-Aldrich). .. This was followed by incubation with a biotin-goat-anti-mouse secondary (1:200; BioLegends, San Diego, Calif), then a fluorescein isothiocyanate-conjugated streptavidin (1:200; Dako).

    Immunostaining:

    Article Title: Airway smooth muscle thickness and contraction are enhanced by intra-amniotic lipopolysaccharide in an ovine model of premature birth.
    Article Snippet: Abnormalities of the airway smooth muscle (ASM) layer in asthma may develop before birth.. We hypothesize that antenatal inflammation causes physiological abnormalities of the ASM that predisposes asthma.. This study determined the short-term effects of antenatal inflammation on the developing ASM.

    Immunohistochemistry:

    Article Title: A novel tropoelastin-based resorbable surgical mesh for pelvic organ prolapse repair
    Article Snippet: .. Immunohistochemistry staining was performed on FFPE sections following antigen retrieval using 0.1 M citrate buffer, blocking endogenous peroxidase with 3% H 2 O 2 , incubation with protein block (Dako) for 30 min at room temperature, using mouse anti-CD45 (0.5 μg/mL, BioRad) and mouse anti-CD206 (0.5 μg/mL, Dendritics) primary antibodies for 1 h at 37C. ..

    Staining:

    Article Title: A novel tropoelastin-based resorbable surgical mesh for pelvic organ prolapse repair
    Article Snippet: .. Immunohistochemistry staining was performed on FFPE sections following antigen retrieval using 0.1 M citrate buffer, blocking endogenous peroxidase with 3% H 2 O 2 , incubation with protein block (Dako) for 30 min at room temperature, using mouse anti-CD45 (0.5 μg/mL, BioRad) and mouse anti-CD206 (0.5 μg/mL, Dendritics) primary antibodies for 1 h at 37C. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: A novel tropoelastin-based resorbable surgical mesh for pelvic organ prolapse repair
    Article Snippet: .. Immunohistochemistry staining was performed on FFPE sections following antigen retrieval using 0.1 M citrate buffer, blocking endogenous peroxidase with 3% H 2 O 2 , incubation with protein block (Dako) for 30 min at room temperature, using mouse anti-CD45 (0.5 μg/mL, BioRad) and mouse anti-CD206 (0.5 μg/mL, Dendritics) primary antibodies for 1 h at 37C. ..

    Labeling:

    Article Title: CCR2-targeting pepducins reduce T cell-nociceptor interaction driving bone cancer pain
    Article Snippet: .. Sections were labeled with mouse anti-pERK (1:100, Cell Signaling #9101), mouse anti-CD45 (1:500, Bio-Rad #MCA43R), mouse anti-CD68 (1:500, Bio-Rad #MCA341R), mouse anti-CD11b (1:500, Millipore, #CBL1512), and mouse anti-NeuN (1:500, Millipore #MAB377), rabbit anti-CCL2 (1:100, PeproTech #500-P76), rabbit anti-connexin 43 (1:500, Sigma #6219), rabbit anti-GFAP (1:500, Sigma, #G3893), rabbit anti-Iba1 (1:500, Wako #019–19741), rabbit anti-pP38 (1:100, Cell Signaling, #4511), rabbit anti-Ki67 (1:100, Abcam #AB16667), and mouse anti-pJNK (1:100, Cell Signaling #9251), chicken anti-CCR2 (1:200, Aves Labs, custom) and chicken anti-CGRP (1:500, Millipore #AB5705), guinea-pig anti-substance P (1:500, Neuromics #GP14110), hamster anti-CD3 (1:500, Santa Cruz, #sc-1174) in blocking buffer for three days at 37°C for spinal cord, 24 hour at R.T. for DRG. .. They were further incubated with fluorophore-conjugated secondary antibodies (1:500, AlexaFluor 488, 568, 647, Invitrogen) and spinal cord sections were mounted on SuperFrost Plus slides.

    Article Title: Discovery of a CCR2-targeting pepducin therapy for chronic pain.
    Article Snippet: .. Sections were labeled with mouse antipERK (1:100, Cell Signaling #9101), mouse anti-CD45 (1:500, BioRad #MCA43R), mouse anti-CD68 (1:500, Bio-Rad #MCA341R), mouse anti-CD11b (1:500, Millipore, #CBL1512), and mouse anti-NeuN (1:500, Millipore #MAB377), rabbit anti-CCL2 (1:100, PeproTech #500-P76), rabbit anti-connexin 43 (1:500, Sigma #6219), rabbit antiGFAP (1:500, Sigma, #G3893), rabbit anti-Iba1 (1:500, Wako #019–19741), rabbit anti-pP38 (1:100, Cell Signaling, #4511), rabbit anti-Ki67 (1:100, Abcam #AB16667), and mouse anti-pJNK (1:100, Cell Signaling #9251), chicken anti-CCR2 (1:200, Aves Labs, custom) and chicken anti-CGRP (1:500, Millipore #AB5705), guinea-pig anti-substance P (1:500, Neuromics #GP14110), hamster anti-CD3 (1:500, Santa Cruz, #sc-1174) in blocking buffer for three days at 37◦C for spinal cord, 24 hour at R.T. for DRG. .. They were further incubated with fluorophoreconjugated secondary antibodies (1:500, AlexaFluor 488, 568, 647, Invitrogen) and spinal cord sections were mounted on SuperFrost Plus slides.



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    Image Search Results


    Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.

    Journal: Glia

    Article Title: Astrocytic EAAT ‐2 Upregulation Supports Neuro‐Axonal Homeostasis in Chronic Inflammatory CNS Lesions

    doi: 10.1002/glia.70243

    Figure Lengend Snippet: Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.

    Article Snippet: CD45 , PE , 30F11 , Miltenyi Biotec , 130‐117‐498 , Rat , ms , 1:20.

    Techniques: Immunofluorescence, Staining, Tissue