hard set mounting media (Vector Laboratories)
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Hard Set Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mounting+media/VECTASHIELD+Vibrance+Antifade+Mounting+Medium/pmc13045554-130-17-21
Average 99 stars, based on 883 article reviews
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Microscopy:Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation. Article Snippet: Cells were then washed with PBS three times and stained with LysoTrackerTM Red DND-99 (cat# L7528, ThermoFisher) for 1 hour followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides and nuclei were stained with DAPI-containing Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation Article Snippet: Cells were then washed with PBS three times and stained with LysoTracker TM Red DND-99 (cat# L7528, ThermoFisher) for 1 h, followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides, and nuclei were stained with DAPI-containing Article Title: Non-viral vasculogenic reprogramming restores cognition and mitigates pathology in Alzheimer’s disease Article Snippet: When required Lycopersicon Esculentum Lectin (LEL, TL) conjugated to DyLight® 48 at 1:20 was used label blood vessels (Vector Labs, Cat. DL-1174-1). .. This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. D3571) and mounted in charged microscope slide using Staining:Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation. Article Snippet: Cells were then washed with PBS three times and stained with LysoTrackerTM Red DND-99 (cat# L7528, ThermoFisher) for 1 hour followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides and nuclei were stained with DAPI-containing Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation Article Snippet: Cells were then washed with PBS three times and stained with LysoTracker TM Red DND-99 (cat# L7528, ThermoFisher) for 1 h, followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides, and nuclei were stained with DAPI-containing Incubation:Article Title: Non-viral vasculogenic reprogramming restores cognition and mitigates pathology in Alzheimer’s disease Article Snippet: When required Lycopersicon Esculentum Lectin (LEL, TL) conjugated to DyLight® 48 at 1:20 was used label blood vessels (Vector Labs, Cat. DL-1174-1). .. This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. D3571) and mounted in charged microscope slide using |
![Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0342/pmc12810342/pmc12810342__gr3.jpg)