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hard set mounting media  (Vector Laboratories)


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    Structured Review

    Vector Laboratories hard set mounting media
    Hard Set Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mounting+media/VECTASHIELD+Vibrance+Antifade+Mounting+Medium/pmc13045554-130-17-21
    Average 99 stars, based on 883 article reviews
    hard set mounting media - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation.
    Article Snippet: Cells were then washed with PBS three times and stained with LysoTrackerTM Red DND-99 (cat# L7528, ThermoFisher) for 1 hour followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides and nuclei were stained with DAPI-containing mounting media (cat# H180010, VectorLabs). .. Images were visualized at 40x (oil immersion lens) using a Zeiss Observer Z1 inverted phase contrast fluorescence microscope (Carl Zeiss Microscopy), and fluorescent intensity was quantified using ImageJ FIJI software (National Institutes of Health).

    Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation
    Article Snippet: Cells were then washed with PBS three times and stained with LysoTracker TM Red DND-99 (cat# L7528, ThermoFisher) for 1 h, followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides, and nuclei were stained with DAPI-containing mounting media (cat# H180010, VectorLabs). .. Images were visualized at 40× (oil immersion lens) using a Zeiss Observer Z1 inverted phase contrast fluorescence microscope (Carl Zeiss Microscopy), and fluorescent intensity was quantified using ImageJ FIJI software (National Institutes of Health).

    Article Title: Non-viral vasculogenic reprogramming restores cognition and mitigates pathology in Alzheimer’s disease
    Article Snippet: When required Lycopersicon Esculentum Lectin (LEL, TL) conjugated to DyLight® 48 at 1:20 was used label blood vessels (Vector Labs, Cat. DL-1174-1). .. This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. D3571) and mounted in charged microscope slide using mounting media (Vector Labs, Cat. H-1700). ..

    Staining:

    Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation.
    Article Snippet: Cells were then washed with PBS three times and stained with LysoTrackerTM Red DND-99 (cat# L7528, ThermoFisher) for 1 hour followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides and nuclei were stained with DAPI-containing mounting media (cat# H180010, VectorLabs). .. Images were visualized at 40x (oil immersion lens) using a Zeiss Observer Z1 inverted phase contrast fluorescence microscope (Carl Zeiss Microscopy), and fluorescent intensity was quantified using ImageJ FIJI software (National Institutes of Health).

    Article Title: ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementation
    Article Snippet: Cells were then washed with PBS three times and stained with LysoTracker TM Red DND-99 (cat# L7528, ThermoFisher) for 1 h, followed by washing with PBS, fixation in 4% paraformaldehyde. .. Coverslips were then mounted on microscope slides, and nuclei were stained with DAPI-containing mounting media (cat# H180010, VectorLabs). .. Images were visualized at 40× (oil immersion lens) using a Zeiss Observer Z1 inverted phase contrast fluorescence microscope (Carl Zeiss Microscopy), and fluorescent intensity was quantified using ImageJ FIJI software (National Institutes of Health).

    Incubation:

    Article Title: Non-viral vasculogenic reprogramming restores cognition and mitigates pathology in Alzheimer’s disease
    Article Snippet: When required Lycopersicon Esculentum Lectin (LEL, TL) conjugated to DyLight® 48 at 1:20 was used label blood vessels (Vector Labs, Cat. DL-1174-1). .. This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. D3571) and mounted in charged microscope slide using mounting media (Vector Labs, Cat. H-1700). ..



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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Servicebio Inc anti fluorescence quenching mounting media
    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Image Search Results


    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Journal: Journal of Translational Autoimmunity

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

    doi: 10.1016/j.jtauto.2025.100345

    Figure Lengend Snippet: Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Article Snippet: Following application of TrueView autofluorescence quencher, slides were mounted using Vectashield Vibrance Antifade mounting media with DAPI (SP-8500-15; Vector Laboratories; USA) and air dried for 2 h before imaging on the Nikon Ti2-E microscope.

    Techniques: Fluorescence