modifier subunit  (Proteintech)

Journal: BMC Veterinary Research

Article Title: The dual action of astaxanthin: activating the Nrf2 pathway and suppressing NF-κB to combat AFB1-induced liver injury in broilers

doi: 10.1186/s12917-026-05330-0

Figure Lengend Snippet: AST attenuated AFB1-induced oxidative stress damage in the liver through the Nrf2 pathway. A Immunoblotting analysis of the protein expression of Keap1, Nrf2, HO-1, NQO1, GCLC and GCLM. The images were merely cropped from the original exposure picture to remove the meaningless parts. B - G The relative levels of the target proteins are shown as histograms representing density readings of the gel bands, and the ratios were calculated relative to the β-actin control. Different capital letters represent P < 0.05

Article Snippet: Antibodies to HO-1, NAD(P)H quinone dehydrogenase 1 (NQO1), keap-1, glutamate-cysteine ligase catalytic subunit (GCLC), the modifier subunit (GCLM), P65, P-P65, and IκBα (Proteintech, Boston, MA, USA).

Techniques: Western Blot, Expressing, Control

Journal: Food Science & Nutrition

Article Title: Fucoxanthin Ameliorates Carbon Tetrachloride‐Induced Liver Fibrosis in Mice via Nrf2/ HO ‐1/ GPX4 ‐Mediated Ferroptosis Pathway

doi: 10.1002/fsn3.70589

Figure Lengend Snippet: Effect of Fx on Nrf2 and ferroptosis signaling pathways in the liver of CCl 4 ‐induced mice. (A) Protein expression of hepatic Nrf2, heme oxygenase‐1 (HO‐1), nicotinamide quinone oxidoreductase 1 (NQO1), and glutamate‐cysteine ligase modifier subunit (GCLM) expression. (B) Protein expression of hepatic GPX4, solute carrier family 7 member 11 (SLC7A11), transferrin receptor 1 (TFR1), and ferritin light chain (FTL). Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 and *** p < 0.001 versus CCl 4 group. ### p < 0.001 between the L‐Fx and H‐Fx groups.

Article Snippet: The following primary antibodies were added to the polyvinylidene fluoride membranes: Nrf2 (1: 2000), GPX4 (1:1000), alpha‐smooth muscle actin (α‐SMA) (14395‐1‐AP, 1:2000), solute carrier family 7 member 11 (SLC7A11) (26864‐1‐AP, 1:2000), NAD(P)H:quinone oxidoreductase 1 (NQO1) (67240‐1‐Ig, 1:2000), transferrin receptor 1 (TFR1) (10084‐2‐AP, 1:2000), glutamate‐cysteine ligase modifier subunit (GCLM) (14241‐1‐AP, 1:2000), ferritin light chain (FTL) (10727‐1‐AP, 1:2000), and heme oxygenase‐1 (HO‐1) (66743‐1‐Ig, 1:2000) (Proteintech Inc., Princeton, NJ, USA).

Techniques: Protein-Protein interactions, Expressing