Review



dulbecco s modified eagle s medium dmem  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC dulbecco s modified eagle s medium dmem
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dulbecco S Modified Eagle S Medium Dmem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 21621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/pmc13678496-245-23-18
    Average 99 stars, based on 21621 article reviews
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Design and synthesis of hydrolyzable coumarin–naphthalimide dual probes for NIR fluorescence detection and selective photodynamic activity in cancer cells"

    Article Title: Design and synthesis of hydrolyzable coumarin–naphthalimide dual probes for NIR fluorescence detection and selective photodynamic activity in cancer cells

    Journal: RSC Advances

    doi: 10.1039/d6ra08240h

    Evaluation of the aqueous compatibility of the C-NAF derivatives in DMEM. UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in Dulbecco's Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Figure Legend Snippet: Evaluation of the aqueous compatibility of the C-NAF derivatives in DMEM. UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in Dulbecco's Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.

    Techniques Used: Modification

    Related Articles

    Cell Culture:

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging
    Article Snippet: .. Hs 675.T human colon fibroblasts (ATCC; CRL-7400) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; ATCC, 30-2002) supplemented with 10% fetal bovine serum (FBS; ATCC, 30-2020), 1% penicillin-streptomycin (ATCC, 30-2300), 5 μg/mL insulin (Lonza Biosciences, BE02-033E20), and 10 ng/mL FGF-2 (R&D Systems, BT-FGFBHS-010). ..

    Article Title: GDF15 (Growth/Differentiation Factor-15) Expression in Human Adipose Tissue and in Adipocyte Cell Lines
    Article Snippet: .. Being cultured at 37 ◦C and 5% CO2 in Dulbecco’s Modified Eagle Medium (ATCC, Manassas, VA, USA; cat. 30-2002) supplemented with 10% newborn calf serum (Sigma-Aldrich, Deisenhofen, Germany; cat. N4762), https://doi.org/10.3390/biomedicines14061329 the cells were incubated in DMEM/F12/glutamate medium (PAN-Biotech, Aidenbach, Germany; cat. P04-41250) supplemented with 20 μM 3-isobutyl-methyl-xanthine (IBMX; Serva, Heidelberg, Germany; cat. 26445), 1 μM corticosterone (Sigma-Aldrich; cat. C2505), 100 nM insulin (Sigma-Aldrich; cat. I6634), 200 μM ascorbate (Sigma-Aldrich; cat. A92902), 2 μg/mL transferrin (Sigma-Aldrich; cat. T1428), 5% fetal calf serum (FCS; Sigma-Aldrich; cat. F6765), 1 μM biotin (Sigma-Aldrich; cat. B4639), 17 μM pantothenate (Sigma-Aldrich; cat. P5155), 100 nM rosiglitazone (Sigma Aldrich; cat. R2408), and 300 μg/mL fetuin (Sigma-Aldrich; cat. F3385) for adipocyte differentiation. ..

    Article Title: Evidence for a Tumor-Suppressive Role of SHP-1 in EMT Regulation in Bladder Cancer Cells.
    Article Snippet: .. Human BCa cell lines 5637, BC16.1, CUBIII, EJ, HT-1376, Hu456, J82, KK-47, LUCC2, MGH-U1, PSI, RT-112, RT-4, SW780, T24, TCC92-1, TCCSUP, UM-UC-3, and UM-UC-14 (ATCC and kind gifts from Dr. C. Reznikoff) were cultured under standard conditions (37 ◦C, 5% CO2) in Dulbecco’s Modified Eagle Medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum, penicillin/streptomycin, and L-glutamine. .. The cell lines were treated prophylactically for mycoplasma using BM-Cyclin (Sigma-Aldrich, St. Louis, MO, USA) once thawed and monitored routinely by DAPI staining but were not independently authenticated.

    Article Title: Mussel-Inspired Catechol-Functionalized Redox-Active Polypeptides for Energy Applications.
    Article Snippet: .. Mouse fibroblast cells (NIH/3T3) were purchased from ATCC (Manassas, VA) and cultured in 5% CO2 at 37 °C in Dulbecco’s Modified Eagle Medium (DMEM, ATCC) supplemented with 10% bovine calf serum and 1% Penicillin−Streptomycin (Sigma-Aldrich, St. Louis, MO) at Penicillin 100 U/mL and streptomycin at 100 mg/mL as the final concentrations. ..

    Modification:

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging
    Article Snippet: .. Hs 675.T human colon fibroblasts (ATCC; CRL-7400) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; ATCC, 30-2002) supplemented with 10% fetal bovine serum (FBS; ATCC, 30-2020), 1% penicillin-streptomycin (ATCC, 30-2300), 5 μg/mL insulin (Lonza Biosciences, BE02-033E20), and 10 ng/mL FGF-2 (R&D Systems, BT-FGFBHS-010). ..

    Article Title: GDF15 (Growth/Differentiation Factor-15) Expression in Human Adipose Tissue and in Adipocyte Cell Lines
    Article Snippet: .. Being cultured at 37 ◦C and 5% CO2 in Dulbecco’s Modified Eagle Medium (ATCC, Manassas, VA, USA; cat. 30-2002) supplemented with 10% newborn calf serum (Sigma-Aldrich, Deisenhofen, Germany; cat. N4762), https://doi.org/10.3390/biomedicines14061329 the cells were incubated in DMEM/F12/glutamate medium (PAN-Biotech, Aidenbach, Germany; cat. P04-41250) supplemented with 20 μM 3-isobutyl-methyl-xanthine (IBMX; Serva, Heidelberg, Germany; cat. 26445), 1 μM corticosterone (Sigma-Aldrich; cat. C2505), 100 nM insulin (Sigma-Aldrich; cat. I6634), 200 μM ascorbate (Sigma-Aldrich; cat. A92902), 2 μg/mL transferrin (Sigma-Aldrich; cat. T1428), 5% fetal calf serum (FCS; Sigma-Aldrich; cat. F6765), 1 μM biotin (Sigma-Aldrich; cat. B4639), 17 μM pantothenate (Sigma-Aldrich; cat. P5155), 100 nM rosiglitazone (Sigma Aldrich; cat. R2408), and 300 μg/mL fetuin (Sigma-Aldrich; cat. F3385) for adipocyte differentiation. ..

    Article Title: A cytidine deaminase biodegrader sensitises multiple cancer types to deoxycytidine analogue-based chemotherapies
    Article Snippet: .. HeLa-S3 (ATCC, CCL-2.2), HEK293T (ATCC, CR-1573) and MIA PaCa2 (ATCC, CRL-1420) were grown in Dulbecco’s Modified Eagle Medium (DMEM). .. AsPC-1 (ATCC, CRL-1682), BxPC-3 (ATCC, CRL-1687), Capan-2 (ATCC, HTB-80), PC-9, HCC4006, H441 (ATCC, HTB−174) and BEAS-2B were grown in Roswell Park Memorial Institute (RPMI) medium.

    Article Title: Evidence for a Tumor-Suppressive Role of SHP-1 in EMT Regulation in Bladder Cancer Cells.
    Article Snippet: .. Human BCa cell lines 5637, BC16.1, CUBIII, EJ, HT-1376, Hu456, J82, KK-47, LUCC2, MGH-U1, PSI, RT-112, RT-4, SW780, T24, TCC92-1, TCCSUP, UM-UC-3, and UM-UC-14 (ATCC and kind gifts from Dr. C. Reznikoff) were cultured under standard conditions (37 ◦C, 5% CO2) in Dulbecco’s Modified Eagle Medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum, penicillin/streptomycin, and L-glutamine. .. The cell lines were treated prophylactically for mycoplasma using BM-Cyclin (Sigma-Aldrich, St. Louis, MO, USA) once thawed and monitored routinely by DAPI staining but were not independently authenticated.

    Article Title: Mussel-Inspired Catechol-Functionalized Redox-Active Polypeptides for Energy Applications.
    Article Snippet: .. Mouse fibroblast cells (NIH/3T3) were purchased from ATCC (Manassas, VA) and cultured in 5% CO2 at 37 °C in Dulbecco’s Modified Eagle Medium (DMEM, ATCC) supplemented with 10% bovine calf serum and 1% Penicillin−Streptomycin (Sigma-Aldrich, St. Louis, MO) at Penicillin 100 U/mL and streptomycin at 100 mg/mL as the final concentrations. ..

    Article Title: Targeting REV7 exposes a mitotic vulnerability in colorectal cancer cells
    Article Snippet: HCT116 (male-derived colorectal carcinoma) and HT-29 (female-derived colorectal adenocarcinoma) cells were obtained from ATCC and maintained in McCoy’s 5A medium (ATCC) supplemented with 10% ( v/v ) fetal bovine serum (FBS; Gibco) and 1% penicillin– streptomycin (Gibco). .. Mouse embryonic fibroblasts (MEFs) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; ATCC) supplemented with 10% ( v/v ) FBS (Gibco) and 1% penicillin–streptomycin (Gibco). ..

    Article Title: Selective YAP1 Transcriptional Response to Graded Retinoic Acid Exposure in NTERA-2 Cells
    Article Snippet: .. Human NTERA-2 cells (NTERA-2 cl.D1; ATCC CRL-1973, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM L-glutamine (Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL penicillin–streptomycin (Capricorn Scientific, Ebsdorfergrund, Germany), and 1% amphotericin B (Biological Industries, Beit HaEmek, Israel). ..

    Incubation:

    Article Title: GDF15 (Growth/Differentiation Factor-15) Expression in Human Adipose Tissue and in Adipocyte Cell Lines
    Article Snippet: .. Being cultured at 37 ◦C and 5% CO2 in Dulbecco’s Modified Eagle Medium (ATCC, Manassas, VA, USA; cat. 30-2002) supplemented with 10% newborn calf serum (Sigma-Aldrich, Deisenhofen, Germany; cat. N4762), https://doi.org/10.3390/biomedicines14061329 the cells were incubated in DMEM/F12/glutamate medium (PAN-Biotech, Aidenbach, Germany; cat. P04-41250) supplemented with 20 μM 3-isobutyl-methyl-xanthine (IBMX; Serva, Heidelberg, Germany; cat. 26445), 1 μM corticosterone (Sigma-Aldrich; cat. C2505), 100 nM insulin (Sigma-Aldrich; cat. I6634), 200 μM ascorbate (Sigma-Aldrich; cat. A92902), 2 μg/mL transferrin (Sigma-Aldrich; cat. T1428), 5% fetal calf serum (FCS; Sigma-Aldrich; cat. F6765), 1 μM biotin (Sigma-Aldrich; cat. B4639), 17 μM pantothenate (Sigma-Aldrich; cat. P5155), 100 nM rosiglitazone (Sigma Aldrich; cat. R2408), and 300 μg/mL fetuin (Sigma-Aldrich; cat. F3385) for adipocyte differentiation. ..



    Similar Products

    99
    ATCC dulbecco s modified eagle s medium dmem
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dulbecco S Modified Eagle S Medium Dmem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/pmc13678496-245-23-18
    Average 99 stars, based on 1 article reviews
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beijing Solarbio Science dulbecco s modified eagle s medium dmem
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dulbecco S Modified Eagle S Medium Dmem, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Medium/pm42914422-45-15-28
    Average 99 stars, based on 1 article reviews
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beijing Solarbio Science dulbecco s modified eagle medium dmem
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dulbecco S Modified Eagle Medium Dmem, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Medium/pm42574815-41-67-98
    Average 99 stars, based on 1 article reviews
    dulbecco s modified eagle medium dmem - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    medium  (ATCC)
    99
    ATCC medium
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/pm42882458-87-7-9
    Average 99 stars, based on 1 article reviews
    medium - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    ATCC dulbecco
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dulbecco, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/pm42874584-915-10-6
    Average 99 stars, based on 1 article reviews
    dulbecco - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    dmem  (ATCC)
    99
    ATCC dmem
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dmem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/pm42858863-54-12-8
    Average 99 stars, based on 1 article reviews
    dmem - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    ATCC dulbeccos minimum essential medium
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Dulbeccos Minimum Essential Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/pm42851157-159-5-10
    Average 99 stars, based on 1 article reviews
    dulbeccos minimum essential medium - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beijing Solarbio Science modified eagle medium
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Modified Eagle Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Medium/pm42858589-51-6-10
    Average 99 stars, based on 1 article reviews
    modified eagle medium - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    ATCC fetal bovine serum
    Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
    Fetal Bovine Serum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium/Dulbecco's+Modified+Eagle's+Medium/10__1158_slash_1535___7163__mct___26___0239-43-19-23
    Average 99 stars, based on 1 article reviews
    fetal bovine serum - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Evaluation of the aqueous compatibility of the C-NAF derivatives in DMEM. UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in Dulbecco's Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.

    Journal: RSC Advances

    Article Title: Design and synthesis of hydrolyzable coumarin–naphthalimide dual probes for NIR fluorescence detection and selective photodynamic activity in cancer cells

    doi: 10.1039/d6ra08240h

    Figure Lengend Snippet: Evaluation of the aqueous compatibility of the C-NAF derivatives in DMEM. UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in Dulbecco's Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.

    Article Snippet: Human embryonic kidney cells HEK-293 (ATCC CRL-1573), used as a healthy model, and MCF-7 adenocarcinoma breast cancer cells (ATCC HTB-22) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U mL −1 penicillin, and 100 μg mL −1 streptomycin at 37 °C, 5% CO 2 , and 100% humidity.

    Techniques: Modification