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mock community  (Zymo Research)


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    Zymo Research mock community
    Mock Community, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mock+community/Positive+Control/pmc13062228-179-11-13
    Average 96 stars, based on 399 article reviews
    mock community - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Extraction:

    Article Title: Shaped by Fire: Unravelling the Impact of Fire on Lizard Gut Microbiome.
    Article Snippet: In the laboratory, DNA was extracted from 237 swabs using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, including extraction controls (n = 9). .. Individual libraries for bacterial communities, including a bacterial mock community (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, USA), extraction and PCR controls, were prepared using a standard dual indexing of the V4 region of the 16S rRNA gene (∼250 bp) with the primers 515F (5′- GTGCCAGCMGCCGCGGTAA- 3′) and 806R (5′- GGACTACHVGGGTWTCTAAT- 3′), following the protocol of Kozich et al. (2013). ..

    Article Title: Shaped by Fire: Unravelling the Impact of Fire on Lizard Gut Microbiome
    Article Snippet: In the laboratory, DNA was extracted from 237 swabs using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, including extraction controls ( n = 9). .. Individual libraries for bacterial communities, including a bacterial mock community (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, USA), extraction and PCR controls, were prepared using a standard dual indexing of the V4 region of the 16S rRNA gene (∼250 bp) with the primers 515F (5′‐GTGCCAGCMGCCGCGGTAA‐3′) and 806R (5′‐GGACTACHVGGGTWTCTAAT‐3′), following the protocol of Kozich et al. ( ). ..

    Polymerase Chain Reaction:

    Article Title: Shaped by Fire: Unravelling the Impact of Fire on Lizard Gut Microbiome.
    Article Snippet: In the laboratory, DNA was extracted from 237 swabs using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, including extraction controls (n = 9). .. Individual libraries for bacterial communities, including a bacterial mock community (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, USA), extraction and PCR controls, were prepared using a standard dual indexing of the V4 region of the 16S rRNA gene (∼250 bp) with the primers 515F (5′- GTGCCAGCMGCCGCGGTAA- 3′) and 806R (5′- GGACTACHVGGGTWTCTAAT- 3′), following the protocol of Kozich et al. (2013). ..

    Article Title: Shaped by Fire: Unravelling the Impact of Fire on Lizard Gut Microbiome
    Article Snippet: In the laboratory, DNA was extracted from 237 swabs using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, including extraction controls ( n = 9). .. Individual libraries for bacterial communities, including a bacterial mock community (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, USA), extraction and PCR controls, were prepared using a standard dual indexing of the V4 region of the 16S rRNA gene (∼250 bp) with the primers 515F (5′‐GTGCCAGCMGCCGCGGTAA‐3′) and 806R (5′‐GGACTACHVGGGTWTCTAAT‐3′), following the protocol of Kozich et al. ( ). ..

    other:

    Article Title: Effect of Systemic Therapies on Skin Bacteriome in Patients with Atopic Dermatitis: A Pilot Prospective Study.
    Article Snippet: Matrix-assisted laser desorption ionization-time of flight mass spectrometry measurements were carried out on a MALDI www.jidonline.org 10.e1 BioTyper system (Bruker Daltonics) and FlexControl 3.4 software (Bruker Daltonics).

    DNA Extraction:

    Article Title: Impact of DNA Extraction and 16S rRNA Gene Amplification Strategy on Microbiota Profiling of Faecal Samples
    Article Snippet: .. The accuracy and bias of each DNA extraction method was assessed using a commercially available MOCK community (ZymoBIOMICS Microbial Community Standard, Catalogue no. D6300, Zymo Research Corp., Irvine, CA, USA). ..

    Positive Control:

    Article Title: Farming system shapes rhizosphere microbiota and root gene expression in common bean
    Article Snippet: .. Negative controls were included to detect potential environmental contaminants, and a Mock Community (Zymo Research, California, USA) was used as a positive control. .. Library preparation and paired-end sequencing (2 × 300 bp) were performed on an Illumina MiSeq platform by Microomics Systems S.L.

    Article Title: Coexistence of methanogenesis and sulfate reduction in a sulfate-adapted enrichment culture from an oil reservoir
    Article Snippet: .. The negative control consisted of DNA-free water, while the positive control contained a commercial Mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research Europe, Freiburg, Germany). .. The extracted DNA was purified and concentrated using magnetic beads (MagSi-NGSPREP Plus, magtivio) and adjusted to a final volume of 30 μL (Illumina Innovative Technologies Inc., 2013).

    Negative Control:

    Article Title: Coexistence of methanogenesis and sulfate reduction in a sulfate-adapted enrichment culture from an oil reservoir
    Article Snippet: .. The negative control consisted of DNA-free water, while the positive control contained a commercial Mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research Europe, Freiburg, Germany). .. The extracted DNA was purified and concentrated using magnetic beads (MagSi-NGSPREP Plus, magtivio) and adjusted to a final volume of 30 μL (Illumina Innovative Technologies Inc., 2013).

    Amplification:

    Article Title: Decoding microbiome responses to quarantine potato wart disease: first insights into suppression and biocontrol by full-length 16S rRNA gene profiling and functional prediction
    Article Snippet: .. Two amplification kits were tested on a standardized mock community (ZymoBIOMICS D6305, Zymo Research, USA), including the GenomiPhi V3 Ready-To-Go DNA Amplification Kit (Cytiva, USA) and the REPLI-g Single Cell Kit (QIAGEN, Germany) according to the manufacturer’s instructions. .. GenomiPhi V3 was selected for further testing on Canadian DNA samples from potato wart-infested fields (n = 7) at the Avondale substation of Agriculture and Agri-Food Canada’s St. John’s Research and Development Center in Newfoundland and Labrador, Canada.

    DNA Amplification:

    Article Title: Decoding microbiome responses to quarantine potato wart disease: first insights into suppression and biocontrol by full-length 16S rRNA gene profiling and functional prediction
    Article Snippet: .. Two amplification kits were tested on a standardized mock community (ZymoBIOMICS D6305, Zymo Research, USA), including the GenomiPhi V3 Ready-To-Go DNA Amplification Kit (Cytiva, USA) and the REPLI-g Single Cell Kit (QIAGEN, Germany) according to the manufacturer’s instructions. .. GenomiPhi V3 was selected for further testing on Canadian DNA samples from potato wart-infested fields (n = 7) at the Avondale substation of Agriculture and Agri-Food Canada’s St. John’s Research and Development Center in Newfoundland and Labrador, Canada.

    Single Cell:

    Article Title: Decoding microbiome responses to quarantine potato wart disease: first insights into suppression and biocontrol by full-length 16S rRNA gene profiling and functional prediction
    Article Snippet: .. Two amplification kits were tested on a standardized mock community (ZymoBIOMICS D6305, Zymo Research, USA), including the GenomiPhi V3 Ready-To-Go DNA Amplification Kit (Cytiva, USA) and the REPLI-g Single Cell Kit (QIAGEN, Germany) according to the manufacturer’s instructions. .. GenomiPhi V3 was selected for further testing on Canadian DNA samples from potato wart-infested fields (n = 7) at the Avondale substation of Agriculture and Agri-Food Canada’s St. John’s Research and Development Center in Newfoundland and Labrador, Canada.



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    Image Search Results


    For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated Zmock reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.

    Journal: bioRxiv

    Article Title: Nanopore sequencing reaches amplicon sequence variant (ASV) resolution

    doi: 10.64898/2026.02.26.708165

    Figure Lengend Snippet: For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated Zmock reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.

    Article Snippet: We first evaluated the ASVs generated from both PacBio and ONT sequencing of the ZymoBIOMICS mock community (Zmock) (Cat. No. D6305, Zymo Research, USA), which contains eight bacteria and two fungal species ( Supplementary Table S1 ).

    Techniques: Sequencing, Amplification