Journal: Cell Biology and Toxicology
Article Title: Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration
doi: 10.1007/s10565-024-09948-4
Figure Lengend Snippet: Impact of SPP1-positive macrophages on the function of msi-mNPCs. Note: ( A ) Correlation analysis of SPP1 and Macrophage abundance in RNA-seq data; ( B ) Western blot detection of SPP1 expression in Control and msi-IDD mouse IVD tissues; ( C ) Influence of co-culture with oe-NC and oe-SPP1 on the proliferation capacity of msi-mNPCs; ( D ) Influence of co-culture with oe-NC and oe-SPP1 on the apoptosis level of msi-mNPCs, with representative images on the left and quantification on the right; ( E – G ) Effect of oe-NC and oe-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( H - I ) Influence of co-culture with sh-NC and sh-SPP1 on the proliferation and apoptosis of msi-mNPCs; ( J - L ) Effect of sh-NC and sh-SPP1 on the secretion of extracellular matrix and inflammatory factors in msi-mNPCs detected by ELISA; ( M – N ) Western blot detection of the impact of oe-SPP1 or sh-SPP1 on the expression of msi-mNPC-related factors; All cell experiments were repeated three times; ns P > 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: The mouse nucleus pulposus cells (mNPCs) (CP-M146, China, provided by Procell) were cultured in Thermo Fisher's DMEM/F-12 (11320033, USA).
Techniques: RNA Sequencing, Western Blot, Expressing, Control, Co-Culture Assay, Enzyme-linked Immunosorbent Assay