Review




Structured Review

Proteintech mmp 3
Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, <t>Aggrecan,</t> <t>MMP-3</t> and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
Mmp 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression"

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

Journal: Non-coding RNA Research

doi: 10.1016/j.ncrna.2025.11.007

Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
Figure Legend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Techniques Used: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing

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Article Snippet: LRRK2 antibody (#A17253) was obtained from Abclonal Antibody (Wuhan, China).

Article Title: cGAS-STING/HMGB1-mediated senescence induced by LRRK2 accelerates cartilage degeneration in osteoarthritis
Article Snippet: Anti-HMGB1 (#CPA7037) was obtained from Cohesion Biosciences (London, UK).

Blocking Assay:

Article Title: Quercetin nanoformulation-embedded hydrogel inhibits osteopontin mediated ferroptosis for intervertebral disc degeneration alleviation.
Article Snippet: An equal amount of protein (50 μg) was separated by SDS-PAGE and transferred onto polyvinylidene fluoride membrane (Millipore, United States). .. After blocking with 5% non-fat milk for 1 h, membranes were respectively incubated with primary antibodies against aggrecan (1: 1000, cat no 13880-1-AP, Proteintech, Wuhan, China, Proteintech, Shanghai, China), MMP-3(1: 1000, cat no 66338-1-Ig, ProteinTech Group, Chicago, IL, USA), NRF2 (1: 1000, cat no 80593-1-RR, ProteinTech Group, Chicago, IL, USA ), FTH1 (1: 1000, cat no 11682- 1-AP, ProteinTech Group, Chicago, IL, USA), GPX4 (1: 1000, cat no 67763-1-Ig, ProteinTech Group, Chicago, IL, USA), OPN(1: 1000, cat no 22952-1-AP, ProteinTech Group, Chicago, IL, USA), β-actin at 4 °C overnight. ..

Incubation:

Article Title: Quercetin nanoformulation-embedded hydrogel inhibits osteopontin mediated ferroptosis for intervertebral disc degeneration alleviation.
Article Snippet: An equal amount of protein (50 μg) was separated by SDS-PAGE and transferred onto polyvinylidene fluoride membrane (Millipore, United States). .. After blocking with 5% non-fat milk for 1 h, membranes were respectively incubated with primary antibodies against aggrecan (1: 1000, cat no 13880-1-AP, Proteintech, Wuhan, China, Proteintech, Shanghai, China), MMP-3(1: 1000, cat no 66338-1-Ig, ProteinTech Group, Chicago, IL, USA), NRF2 (1: 1000, cat no 80593-1-RR, ProteinTech Group, Chicago, IL, USA ), FTH1 (1: 1000, cat no 11682- 1-AP, ProteinTech Group, Chicago, IL, USA), GPX4 (1: 1000, cat no 67763-1-Ig, ProteinTech Group, Chicago, IL, USA), OPN(1: 1000, cat no 22952-1-AP, ProteinTech Group, Chicago, IL, USA), β-actin at 4 °C overnight. ..

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression
Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies. .. HE, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and IHC stained sections were scanned using a Pannoramic MIDI (3D HISTECH, Hungary), whereas EdU-stained sections were acquired using a BX53 microscope.

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression
Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies. .. HE, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and IHC stained sections were scanned using a Pannoramic MIDI (3D HISTECH, Hungary), whereas EdU-stained sections were acquired using a BX53 microscope.



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Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, <t>Aggrecan,</t> <t>MMP-3</t> and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
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Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, <t>Aggrecan,</t> <t>MMP-3</t> and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
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Image Search Results


The VCA-D4. A) Schematic representation of the VCA-D4 with its three-zone architecture. Zone 1 contains the TAC-D4 competitive assay format while Zones 2 and 3 have a traditional sandwich immunoassay format for MMP3 and IL-6 respectfully. B) VCA-D4 dose response curves for tacrolimus (gray), MMP3 (yellow) and IL-6 (blue) using fetal bovine serum.

Journal: Analytical chemistry

Article Title: A point-of-care test to measure tacrolimus directly from unprocessed whole blood

doi: 10.1021/acs.analchem.5c07803

Figure Lengend Snippet: The VCA-D4. A) Schematic representation of the VCA-D4 with its three-zone architecture. Zone 1 contains the TAC-D4 competitive assay format while Zones 2 and 3 have a traditional sandwich immunoassay format for MMP3 and IL-6 respectfully. B) VCA-D4 dose response curves for tacrolimus (gray), MMP3 (yellow) and IL-6 (blue) using fetal bovine serum.

Article Snippet: For ELISA comparison studies, R&D Systems Quantikine ELISA kits were used for both IL-6 (R&D Systems Catalog Number: D6050) and MMP3 (R&D Systems Catalog Number: DMP300).

Techniques:

Comparison of the performance of the VCA-D4 to commercial ELISAs. A) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA using the ELISA IL-6 standard (R 2 =0.95). B) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA (R 2 =0.97) using the selected IL-6 protein used for VCA-D4 development. C) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA using the ELISA MMP3 standard (R 2 =0.71 when using all points and 0.82 when using points above 0.1 ng/mL–the LOD of the VCA-D4). D) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA (R 2 =0.7 for all points, 0.8 for points above 1 ng/mL) using the selected MMP3 protein used for VCA-D4 development.

Journal: Analytical chemistry

Article Title: A point-of-care test to measure tacrolimus directly from unprocessed whole blood

doi: 10.1021/acs.analchem.5c07803

Figure Lengend Snippet: Comparison of the performance of the VCA-D4 to commercial ELISAs. A) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA using the ELISA IL-6 standard (R 2 =0.95). B) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA (R 2 =0.97) using the selected IL-6 protein used for VCA-D4 development. C) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA using the ELISA MMP3 standard (R 2 =0.71 when using all points and 0.82 when using points above 0.1 ng/mL–the LOD of the VCA-D4). D) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA (R 2 =0.7 for all points, 0.8 for points above 1 ng/mL) using the selected MMP3 protein used for VCA-D4 development.

Article Snippet: For ELISA comparison studies, R&D Systems Quantikine ELISA kits were used for both IL-6 (R&D Systems Catalog Number: D6050) and MMP3 (R&D Systems Catalog Number: DMP300).

Techniques: Comparison, Fluorescence, Enzyme-linked Immunosorbent Assay

Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Journal: Non-coding RNA Research

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

doi: 10.1016/j.ncrna.2025.11.007

Figure Lengend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Article Snippet: Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies.

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing