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mito flipper-tr  (Spirochrome)


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    Spirochrome mito flipper-tr
    Mito Flipper Tr, supplied by Spirochrome, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mito+flipper+tr/Mito+Flipper-TR/custom%40sc023%4042501704
    Average 93 stars, based on 12 article reviews
    mito flipper-tr - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics
    Article Snippet: .. Samples were then incubated with 1 μM Flipper-TR (SC020, Spirochrome), 1 μM ER Flipper-TR (SC021, Spirochrome), 1 μM Mito Flipper-TR (SC023, Spirochrome), or 1 μM Lyso Flipper-TR (SC022, Spirochrome) for 15 min and washed with live-cell imaging solution (A14291DJ, Invitrogen) before imaging. .. Imaging was performed using Nikon A1R with a PicoQuant FCS/FLIM module in Ti-E (inverted).

    Article Title: Matrix stiffening promotes perinuclear clustering of mitochondria
    Article Snippet: Images were acquired with a 60X silicone oil objective (UPLSAPO60XS2, N.A = 1.3, Olympus) mounted on an Olympus IX83 inverted microscope supplied with Yokogawa CSU-W1 (SoRa Disk) scanner. .. For FLIM imaging with Mito Flipper-TR, cells were incubated with 1 μM of the Mito Flipper-TR (Spirochrome) for 15 min. Media conducive for live imaging conditions (fluorobrite DMEM with 10% FBS, 1 mM Glutamine, and 50 μM HEPES buffer) was added to dilute the probe concentration three times and imaging was done in a live cell chamber at 37°C. .. Imaging was performed using the FV3000 Olympus microscope equipped with a time-correlated single-photon counting module from PicoQuant.

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics.
    Article Snippet: .. Samples were then incubated with 1 μM Flipper- TR (SC020, Spirochrome), 1 μM ER Flipper- TR (SC021, Spirochrome), 1 μM Mito Flipper- TR (SC023, Spirochrome), or 1 μM Lyso Flipper- TR (SC022, Spirochrome) for 15 min and washed with live- cell imaging solution (A14291DJ, Invitrogen) before imaging. .. Imaging was performed using Nikon A1R with a PicoQuant FCS/FLIM module in Ti- E (inverted).

    Article Title: Matrix stiffening promotes perinuclear clustering of mitochondria
    Article Snippet: Images were acquired with a 60X silicone oil objective ( UPLSAPO60XS2 , N.A=1.3, Olympus) mounted on an Olympus IX83 inverted microscope supplied with Yokogawa CSU-W1 (SoRa Disk) scanner. .. For FLIM imaging with Mito Flipper-TR, cells were incubated with 1 μM of the Mito Flipper-TR (Spirochrome) for 15 min. Media conducive for live imaging conditions (fluorobrite DMEM with 10 % FBS, 1 mM Glutamine and 50 μM Hepes buffer) was added to dilute the probe concentration three times and imaging was done in a live cell chamber at 37°C. .. Imaging was performed using the FV3000 Olympus microscope equipped with a time-correlated single-photon counting module from PicoQuant.

    Live Cell Imaging:

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics
    Article Snippet: .. Samples were then incubated with 1 μM Flipper-TR (SC020, Spirochrome), 1 μM ER Flipper-TR (SC021, Spirochrome), 1 μM Mito Flipper-TR (SC023, Spirochrome), or 1 μM Lyso Flipper-TR (SC022, Spirochrome) for 15 min and washed with live-cell imaging solution (A14291DJ, Invitrogen) before imaging. .. Imaging was performed using Nikon A1R with a PicoQuant FCS/FLIM module in Ti-E (inverted).

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics.
    Article Snippet: .. Samples were then incubated with 1 μM Flipper- TR (SC020, Spirochrome), 1 μM ER Flipper- TR (SC021, Spirochrome), 1 μM Mito Flipper- TR (SC023, Spirochrome), or 1 μM Lyso Flipper- TR (SC022, Spirochrome) for 15 min and washed with live- cell imaging solution (A14291DJ, Invitrogen) before imaging. .. Imaging was performed using Nikon A1R with a PicoQuant FCS/FLIM module in Ti- E (inverted).

    Imaging:

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics
    Article Snippet: .. Samples were then incubated with 1 μM Flipper-TR (SC020, Spirochrome), 1 μM ER Flipper-TR (SC021, Spirochrome), 1 μM Mito Flipper-TR (SC023, Spirochrome), or 1 μM Lyso Flipper-TR (SC022, Spirochrome) for 15 min and washed with live-cell imaging solution (A14291DJ, Invitrogen) before imaging. .. Imaging was performed using Nikon A1R with a PicoQuant FCS/FLIM module in Ti-E (inverted).

    Article Title: Matrix stiffening promotes perinuclear clustering of mitochondria
    Article Snippet: Images were acquired with a 60X silicone oil objective (UPLSAPO60XS2, N.A = 1.3, Olympus) mounted on an Olympus IX83 inverted microscope supplied with Yokogawa CSU-W1 (SoRa Disk) scanner. .. For FLIM imaging with Mito Flipper-TR, cells were incubated with 1 μM of the Mito Flipper-TR (Spirochrome) for 15 min. Media conducive for live imaging conditions (fluorobrite DMEM with 10% FBS, 1 mM Glutamine, and 50 μM HEPES buffer) was added to dilute the probe concentration three times and imaging was done in a live cell chamber at 37°C. .. Imaging was performed using the FV3000 Olympus microscope equipped with a time-correlated single-photon counting module from PicoQuant.

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics.
    Article Snippet: .. Samples were then incubated with 1 μM Flipper- TR (SC020, Spirochrome), 1 μM ER Flipper- TR (SC021, Spirochrome), 1 μM Mito Flipper- TR (SC023, Spirochrome), or 1 μM Lyso Flipper- TR (SC022, Spirochrome) for 15 min and washed with live- cell imaging solution (A14291DJ, Invitrogen) before imaging. .. Imaging was performed using Nikon A1R with a PicoQuant FCS/FLIM module in Ti- E (inverted).

    Article Title: Matrix stiffening promotes perinuclear clustering of mitochondria
    Article Snippet: Images were acquired with a 60X silicone oil objective ( UPLSAPO60XS2 , N.A=1.3, Olympus) mounted on an Olympus IX83 inverted microscope supplied with Yokogawa CSU-W1 (SoRa Disk) scanner. .. For FLIM imaging with Mito Flipper-TR, cells were incubated with 1 μM of the Mito Flipper-TR (Spirochrome) for 15 min. Media conducive for live imaging conditions (fluorobrite DMEM with 10 % FBS, 1 mM Glutamine and 50 μM Hepes buffer) was added to dilute the probe concentration three times and imaging was done in a live cell chamber at 37°C. .. Imaging was performed using the FV3000 Olympus microscope equipped with a time-correlated single-photon counting module from PicoQuant.

    Concentration Assay:

    Article Title: Matrix stiffening promotes perinuclear clustering of mitochondria
    Article Snippet: Images were acquired with a 60X silicone oil objective (UPLSAPO60XS2, N.A = 1.3, Olympus) mounted on an Olympus IX83 inverted microscope supplied with Yokogawa CSU-W1 (SoRa Disk) scanner. .. For FLIM imaging with Mito Flipper-TR, cells were incubated with 1 μM of the Mito Flipper-TR (Spirochrome) for 15 min. Media conducive for live imaging conditions (fluorobrite DMEM with 10% FBS, 1 mM Glutamine, and 50 μM HEPES buffer) was added to dilute the probe concentration three times and imaging was done in a live cell chamber at 37°C. .. Imaging was performed using the FV3000 Olympus microscope equipped with a time-correlated single-photon counting module from PicoQuant.

    Article Title: Matrix stiffening promotes perinuclear clustering of mitochondria
    Article Snippet: Images were acquired with a 60X silicone oil objective ( UPLSAPO60XS2 , N.A=1.3, Olympus) mounted on an Olympus IX83 inverted microscope supplied with Yokogawa CSU-W1 (SoRa Disk) scanner. .. For FLIM imaging with Mito Flipper-TR, cells were incubated with 1 μM of the Mito Flipper-TR (Spirochrome) for 15 min. Media conducive for live imaging conditions (fluorobrite DMEM with 10 % FBS, 1 mM Glutamine and 50 μM Hepes buffer) was added to dilute the probe concentration three times and imaging was done in a live cell chamber at 37°C. .. Imaging was performed using the FV3000 Olympus microscope equipped with a time-correlated single-photon counting module from PicoQuant.



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    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with <t>Mito</t> Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
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    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with <t>Mito</t> Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
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    Image Search Results


    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.

    Journal: Science Advances

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics

    doi: 10.1126/sciadv.ads6132

    Figure Lengend Snippet: ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.

    Article Snippet: Samples were then incubated with 1 μM Flipper-TR (SC020, Spirochrome), 1 μM ER Flipper-TR (SC021, Spirochrome), 1 μM Mito Flipper-TR (SC023, Spirochrome), or 1 μM Lyso Flipper-TR (SC022, Spirochrome) for 15 min and washed with live-cell imaging solution (A14291DJ, Invitrogen) before imaging.

    Techniques: Fluorescence, Staining