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mircury microarray labeling kit  (Qiagen)


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    Structured Review

    Qiagen mircury microarray labeling kit
    miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a <t>microarray</t> analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.
    Mircury Microarray Labeling Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mircury+microarray+labeling+kit/mircury+microarray+labeling+kit/pmc08595003-62-9-13
    Average 90 stars, based on 1 article reviews
    mircury microarray labeling kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis"

    Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis

    Journal: Analytical Cellular Pathology (Amsterdam)

    doi: 10.1155/2021/4132488

    miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.
    Figure Legend Snippet: miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.

    Techniques Used: Cell Culture, Microarray, Expressing, Quantitative RT-PCR

    Related Articles

    Isolation:

    Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis
    Article Snippet: Total RNA from HCT116 and SW480 cells cultured under hypoxic or normoxic conditions was extracted using the TRIzol reagent (Thermo Fisher Scientific), and the miRNA part was further purified using a mirVana miRNA extraction kit (Ambion, Austin, TX, USA). .. The isolated miRNAs were labeled with Hy3 using a miRCURY microarray labeling kit (Exiqon) and then hybridized using a miRCURY LNA miRNA Array (v.8.0, Exiqon). .. The microarray data were collected using a GenePix 4000B scanner (Axon Instruments, Molecular Devices, San Jose, CA, USA) and analyzed using a GenePix Pro 6.0 software (Axon Instruments).

    Labeling:

    Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis
    Article Snippet: Total RNA from HCT116 and SW480 cells cultured under hypoxic or normoxic conditions was extracted using the TRIzol reagent (Thermo Fisher Scientific), and the miRNA part was further purified using a mirVana miRNA extraction kit (Ambion, Austin, TX, USA). .. The isolated miRNAs were labeled with Hy3 using a miRCURY microarray labeling kit (Exiqon) and then hybridized using a miRCURY LNA miRNA Array (v.8.0, Exiqon). .. The microarray data were collected using a GenePix 4000B scanner (Axon Instruments, Molecular Devices, San Jose, CA, USA) and analyzed using a GenePix Pro 6.0 software (Axon Instruments).

    Microarray:

    Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis
    Article Snippet: Total RNA from HCT116 and SW480 cells cultured under hypoxic or normoxic conditions was extracted using the TRIzol reagent (Thermo Fisher Scientific), and the miRNA part was further purified using a mirVana miRNA extraction kit (Ambion, Austin, TX, USA). .. The isolated miRNAs were labeled with Hy3 using a miRCURY microarray labeling kit (Exiqon) and then hybridized using a miRCURY LNA miRNA Array (v.8.0, Exiqon). .. The microarray data were collected using a GenePix 4000B scanner (Axon Instruments, Molecular Devices, San Jose, CA, USA) and analyzed using a GenePix Pro 6.0 software (Axon Instruments).



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    mircury microarray labeling kit  (Qiagen)
    90
    Qiagen mircury microarray labeling kit
    miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a <t>microarray</t> analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.
    Mircury Microarray Labeling Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mircury+microarray+labeling+kit/mircury+microarray+labeling+kit/pmc08595003-62-9-13
    Average 90 stars, based on 1 article reviews
    mircury microarray labeling kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.

    Journal: Analytical Cellular Pathology (Amsterdam)

    Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis

    doi: 10.1155/2021/4132488

    Figure Lengend Snippet: miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.

    Article Snippet: The isolated miRNAs were labeled with Hy3 using a miRCURY microarray labeling kit (Exiqon) and then hybridized using a miRCURY LNA miRNA Array (v.8.0, Exiqon).

    Techniques: Cell Culture, Microarray, Expressing, Quantitative RT-PCR