elisa kits  (R&D Systems)

Journal: bioRxiv

Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue

doi: 10.64898/2026.04.20.719545

Figure Lengend Snippet: A. Schematic overview of a novel 32-color spectral flow cytometry panel for profiling circulating immune populations and intracellular senescence markers p16 and p21. B. PCA analysis of young and aged peripheral blood mononuclear cells (PBMCs), integrating all identified immune cell subsets. C. IMMAge score calculated based on PBMC subsets for young and aged participants in young (n=8) and aged (n=12) participants. D. CD4+ CD57+CD28- and E. CD8+ CD57+CD28-senescent cells expressed as a percentage of total CD4+ or CD8 T cells in young (n=8) and aged (n=12) participants. Intracellular p21 expression in F. Naïve and G. EMRA CD8+ T cells from (n=9) young and aged (n=12) participants. H. Intracellular p16 expression assessed as median fluorescent intensity in Memory B cells (CD19+ CD38− CD24+) from (n=9) young and aged (n=12) participants. Expression of CD95 in I. CD4+ and J. CD8+ T cells between in young (n=8) and aged (n=12) participants. K. Schematic overview of cryosection and whole-tissue approaches for detecting cellular senescence using β-galactosidase staining (SA-beta gal) in human adipose tissue. Representative images of positive β-galactosidase staining (blue) of senescent cells in L. 20 µM human adipose tissue cryosections and M. whole human adipose tissue following 24h incubation. Expression of SASP markers N. GDF15, O. CXCL-9, P. TNF alpha, and Q. IL-6 in plasma samples from young (n=5) and aged (n=12) participants, determined by ELISA. Data are mean ± S.E.M. Statistical significance was assessed (C-F, H-J) unpaired t-tests or (G, N-Q) Mann Whitney U.

Article Snippet: Serum and EDTA plasma concentration of CXCL-9, GDF-15, IL-6 and TNFα, were measured using commercially available ELISA kits (CXCL9; DY392-05, GDF15; DY957, IL-6; DY206, TNF; DY210, R&D Systems, MN, USA) following the manufacturer’s protocol.

Techniques: Flow Cytometry, Expressing, Staining, Incubation, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: bioRxiv

Article Title: Selective JAK Inhibition Reveals Paradoxical and Hierarchical Control of interferon-γ-driven Autoimmunity in AIRE Deficiency

doi: 10.64898/2026.03.05.709894

Figure Lengend Snippet: Untreated Air e −/− mice and Air e −/− mice treated for four weeks with selective JAK1i, JAK2i, or JAK3i were analyzed. Lungs were processed for intracellular cytokine staining, qPCR, ELISA, and immunoblot analyses. ( A-B ) Representative flow cytometry plots showing IFN-γ production by CD4 + and CD8 + T cells. ( C-D ) Frequency (of total CD4 + and CD8 + T cells) and absolute numbers of IFN-γ + CD4 + and IFN-γ + CD8 + T cells in the lung. ( E-F ) Relative Ifng mRNA expression and IFN-γ protein concentrations in lung homogenates. ( G ) Relative Stat1 mRNA expression. ( H ) Representative immunoblots of phospho-STAT1 (pSTAT1), total STAT1, and GAPDH. ( I-J ) Quantification of total STAT1 and phospho-STAT1 normalized to GAPDH. For IFN- γ + CD4 + and IFN-γ + CD8 + T cells: n = 9-14 mice per group from four independent experiments. For Ifng and Cxcl9 mRNA: n = 15-22 mice per group from four independent experiments. For Stat1 _mRNA: n = 10-17 mice per group from three independent experiments. For CXCL9 protein: n = 5-10 mice per group from two independent experiments. For STAT1 and pSTAT1 immunoblots: n = 15-22 mice per group from four independent experiments). Statistical analyses were performed using one-way ANOVA or Kruskal-Wallis test with Dunn’s multiple comparisons to the untreated Air e −/− mice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: A DuoSet® ELISA kit (catalog no. DY492-05; R&D Systems, USA) was used to measure CXCL9 and a high-sensitivity ELISA kit was used for IFN-γ (catalog no. 88-8314-88; Invitrogen, USA).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Expressing