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microsomal protein content  (Bio-Rad)


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    Bio-Rad microsomal protein content
    Microsomal Protein Content, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 16374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microsomal+protein+content/DC+Protein+Assay+Reagents+Package/pm39496387-142-15-26
    Average 97 stars, based on 16374 article reviews
    microsomal protein content - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Protein Quantitation:

    Article Title: Biochemical mechanism of Caffeic Acid Phenylethyl Ester (CAPE) selective toxicity towards melanoma cell lines
    Article Snippet: .. Microsomal protein content was determined using the Bio-Rad protein assay kit (Cat. No. 500-0006, Hercules, CA) for protein quantitation [ 23 ]. ..

    other:

    Article Title: Statins increase cytochrome P450 4F3-mediated eicosanoids production in human liver cells: a PXR dependent mechanism.
    Article Snippet: In the present study, the ability of lovastatin, a competitive inhibitor of HMG-CoA reductase, to regulate the gene expression and function of Cytochrome P450 4F3B (CYP4F3B) was examined in the well differentiated HepaRG human hepatoma cell line.. Statins induced CYP4F3B mRNA, protein and the production of 20-hydroxyeicosatetraenoic acid (20-HETE), a product of arachidonic acid metabolism and a peroxisome proliferator activated receptor (PPAR) ligand.. This response was not dependent on cholesterol shortage or on sterol regulatory element binding protein activation.

    Suspension:

    Article Title: Perinatal maternal exposure to high-dose sodium phenobarbital in the modified Comparative Thyroid Assay: no significant reduction in thyroid hormones in pups despite notable effects in dams.
    Article Snippet: .. For each microsomal suspension derived from the frozen liv- er samples of the maternal rats, microsomal protein content was determined by DC Protein Assay Kit (Nippon Bio-Rad Laboratories, Tokyo) according to the augmented method of Lowry. .. Total RNA in the liver was extracted from liver from dams and pups using RNeasy Mini kit (Qiagen, Hilden, Germany) in accordance with manufacturer’s instructions.

    Derivative Assay:

    Article Title: Perinatal maternal exposure to high-dose sodium phenobarbital in the modified Comparative Thyroid Assay: no significant reduction in thyroid hormones in pups despite notable effects in dams.
    Article Snippet: .. For each microsomal suspension derived from the frozen liv- er samples of the maternal rats, microsomal protein content was determined by DC Protein Assay Kit (Nippon Bio-Rad Laboratories, Tokyo) according to the augmented method of Lowry. .. Total RNA in the liver was extracted from liver from dams and pups using RNeasy Mini kit (Qiagen, Hilden, Germany) in accordance with manufacturer’s instructions.

    DC Protein Assay:

    Article Title: Perinatal maternal exposure to high-dose sodium phenobarbital in the modified Comparative Thyroid Assay: no significant reduction in thyroid hormones in pups despite notable effects in dams.
    Article Snippet: .. For each microsomal suspension derived from the frozen liv- er samples of the maternal rats, microsomal protein content was determined by DC Protein Assay Kit (Nippon Bio-Rad Laboratories, Tokyo) according to the augmented method of Lowry. .. Total RNA in the liver was extracted from liver from dams and pups using RNeasy Mini kit (Qiagen, Hilden, Germany) in accordance with manufacturer’s instructions.

    Article Title: Protective effects of goldenseal ( Hydrastis canadensis L.) on acetaminophen-induced hepatotoxicity through inhibition of CYP2E1 in rats
    Article Snippet: .. The microsomal protein content was determined by the method of Lowry, using the DC protein assay kit (BIO RAD). ..



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    Image Search Results


    Human hepatic CYP content (pmol/mg microsomal protein) for CYP2C9, CYP2C19, CYP2E1, CYP3A4, CYP3A5 and CYP3A7 plotted against age. For each individual plot, the center of the three lines is the average CYP content while the upper and lower two lines represent the 95% confidence intervals. Equations generated from plots are utilized in the human heapatic CYP content calculator (http://wings.buffalo.edu/smbs/pmy/resources/Cyp%20Calculator.htm).

    Journal: Toxicology

    Article Title: Cytochrome P450-specific human PBPK/PD models for the organophosphorus pesticides: chlorpyrifos and parathion

    doi: 10.1016/j.tox.2011.04.002

    Figure Lengend Snippet: Human hepatic CYP content (pmol/mg microsomal protein) for CYP2C9, CYP2C19, CYP2E1, CYP3A4, CYP3A5 and CYP3A7 plotted against age. For each individual plot, the center of the three lines is the average CYP content while the upper and lower two lines represent the 95% confidence intervals. Equations generated from plots are utilized in the human heapatic CYP content calculator (http://wings.buffalo.edu/smbs/pmy/resources/Cyp%20Calculator.htm).

    Article Snippet: CYP content (pmol P450/mg microsomal protein) was plotted against age (log[days]) using MATLAB (The Mathworks Inc; Natick, MA) to generate curves ( ).

    Techniques: Generated

    A comparison of metabolic parameters for PBPK/PD models published by Timchalk et al. (2002) and the refined human CYP-specific models.

    Journal: Toxicology

    Article Title: Cytochrome P450-specific human PBPK/PD models for the organophosphorus pesticides: chlorpyrifos and parathion

    doi: 10.1016/j.tox.2011.04.002

    Figure Lengend Snippet: A comparison of metabolic parameters for PBPK/PD models published by Timchalk et al. (2002) and the refined human CYP-specific models.

    Article Snippet: CYP content (pmol P450/mg microsomal protein) was plotted against age (log[days]) using MATLAB (The Mathworks Inc; Natick, MA) to generate curves ( ).

    Techniques: Comparison, Activation Assay

    The resulting output for human hepatic CYP content for a 1 year old and 19 year old using the human hepatic CYP content calculator.

    Journal: Toxicology

    Article Title: Cytochrome P450-specific human PBPK/PD models for the organophosphorus pesticides: chlorpyrifos and parathion

    doi: 10.1016/j.tox.2011.04.002

    Figure Lengend Snippet: The resulting output for human hepatic CYP content for a 1 year old and 19 year old using the human hepatic CYP content calculator.

    Article Snippet: CYP content (pmol P450/mg microsomal protein) was plotted against age (log[days]) using MATLAB (The Mathworks Inc; Natick, MA) to generate curves ( ).

    Techniques:

    Human PBPK/PD model simulations of the time course for AChE (solid line) and BuChE (dashed line) inhibition following a single oral exposure to chlorpyrifos or parathion in infants and adults. Average hepatic CYP content for the two ages (Table 2) and protein binding values for chlorpyrifos (Timchalk et al. 2002b) and parathion (Table 3) were utilized.

    Journal: Toxicology

    Article Title: Cytochrome P450-specific human PBPK/PD models for the organophosphorus pesticides: chlorpyrifos and parathion

    doi: 10.1016/j.tox.2011.04.002

    Figure Lengend Snippet: Human PBPK/PD model simulations of the time course for AChE (solid line) and BuChE (dashed line) inhibition following a single oral exposure to chlorpyrifos or parathion in infants and adults. Average hepatic CYP content for the two ages (Table 2) and protein binding values for chlorpyrifos (Timchalk et al. 2002b) and parathion (Table 3) were utilized.

    Article Snippet: CYP content (pmol P450/mg microsomal protein) was plotted against age (log[days]) using MATLAB (The Mathworks Inc; Natick, MA) to generate curves ( ).

    Techniques: Inhibition, Protein Binding

    Effect of varying hepatic CYP content on human PBPK/PD model simulations for a 19yr old following a single oral exposure to chlorpyrifos.

    Journal: Toxicology

    Article Title: Cytochrome P450-specific human PBPK/PD models for the organophosphorus pesticides: chlorpyrifos and parathion

    doi: 10.1016/j.tox.2011.04.002

    Figure Lengend Snippet: Effect of varying hepatic CYP content on human PBPK/PD model simulations for a 19yr old following a single oral exposure to chlorpyrifos.

    Article Snippet: CYP content (pmol P450/mg microsomal protein) was plotted against age (log[days]) using MATLAB (The Mathworks Inc; Natick, MA) to generate curves ( ).

    Techniques: Inhibition

    Effect of varying hepatic CYP content on human PBPK/PD model simulations for a 19yr old following a single oral exposure to parathion.

    Journal: Toxicology

    Article Title: Cytochrome P450-specific human PBPK/PD models for the organophosphorus pesticides: chlorpyrifos and parathion

    doi: 10.1016/j.tox.2011.04.002

    Figure Lengend Snippet: Effect of varying hepatic CYP content on human PBPK/PD model simulations for a 19yr old following a single oral exposure to parathion.

    Article Snippet: CYP content (pmol P450/mg microsomal protein) was plotted against age (log[days]) using MATLAB (The Mathworks Inc; Natick, MA) to generate curves ( ).

    Techniques: Inhibition