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Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

Journal: Breast Cancer Research : BCR

Article Title: Activating transcription factor-2 (ATF2) is a key determinant of resistance to endocrine treatment in an in vitro model of breast cancer

doi: 10.1186/s13058-020-01359-7

Figure Lengend Snippet: Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

Article Snippet: We also performed a microarray gene expression profiling (Illumina Human HT12_v4) to explore alterations in gene expression between MCF7 and TAMRs after ATF2 silencing and confirmed gene expression changes by quantitative RT-PCR.

Techniques: Biomarker Discovery, Microarray, Transfection, Negative Control, Quantitative RT-PCR, Knockdown