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microarray gene expression analysis  (Thermo Fisher)


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    Thermo Fisher microarray gene expression analysis
    Microarray Gene Expression Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+gene+expression+analysis/microarray+gene+expression+analysis/pm38843995-127-1-8
    Average 90 stars, based on 1 article reviews
    microarray gene expression analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Comprehensive genomic profiling of EWSR1/FUS::CREB translocation-associated tumors uncovers prognostically significant recurrent genetic alterations and methylation-transcriptional correlates.
    Article Snippet: .. Affymetrix microarray gene expression analysis Details of the microarray protocol were described previously5,6. .. RNA was isolated using RNAwiz RNA isolation reagent (Ambion) and run through a column with RNase-free DNase (Qiagen).

    Article Title: Diacylglycerol kinase delta overexpression improves glucose clearance and protects against the development of obesity.
    Article Snippet: .. A microarray gene expression analysis was performed by Affymetrix Mouse Gene 1.1 ST array (ThermoFisher Scientific) on the total RNA extracted from EDL muscle wild-type sedentary (WT; n=5), MLC_DGKδ2 transgenic sedentary mice (TG; n=6), wild-type exercised (WT exercised; n=5) and MLC_DGKδ2 transgenic exercised mice (TG exercised; n=5). ..

    Article Title: SLC45A2: A Melanoma Antigen with High Tumor Selectivity and Reduced Potential for Autoimmune Toxicity
    Article Snippet: .. Three days following transduction, GFP-positive melanocytes were sorted and RNA was prepared for Affymetrix microarray gene expression analysis as described previously ( 22 ). ..

    Article Title: Thyroid Hormone Transporters MCT8 and OATP1C1 Control Skeletal Muscle Regeneration
    Article Snippet: .. To obtain a broad overview of the expression pattern of different TH hormone transporters ( ) during myogenesis, we took advantage of our previously published Affymetrix microarray gene expression analysis ( ). ..

    Article Title: Time-course analysis of microRNA-induced mesenchymal-to-epithelial transition underscores the complexity of the underlying molecular processes.
    Article Snippet: .. Three biologically independent RNA samples were collected at each time point (21 samples total) and subjected to microarray gene expression analysis (Affymetrix, U133 +2) as previously described [15]. .. Initial quality control, summarization and normalization of all 21 CEL files using RMA (Robust Multiarray Average) was performed using the Affymetrix Expression Console (software 1.2.1.20) resulting in expression data for 54,613 probe sets (23,518 annotated genes).

    Article Title: TransCONFIRM: Identification of a genetic signature of response to fulvestrant in advanced hormone receptor positive breast cancer
    Article Snippet: .. The PAM50 intrinsic subtype predictions and RS classifications predictions in this study are considered surrogate classifiers since they are based on Affymetrix microarray gene expression analysis and not the original platforms that were used to establish these classifiers (Microarray data from Agilent human 1Av2 microarrays for the PAM50 and RT-PCR for OncotypeDx RS). .. Immunohistochemistry Studies Samples were centrally stained for ER, PR (ER/PR pharmDxTM Kit, Dako), HER2 (HercepTestTM Kit, Dako) and Ki67 (clone MIB-1, dilution 1:100, DAKO).

    Article Title: Transforming Growth Factor β‐Activated Kinase 1 Regulates Mesenchymal Stem Cell Proliferation Through Stabilization of Yap1/Taz Proteins
    Article Snippet: .. The microarray gene expression analysis of DMSO treated BMMSCs and 5zox treated BMMSCs were performed using the Affymetrix ClariomTM S, mouse (Thermo Fisher Scientific) and the Affymetrix Transcriptome Analysis Console software (Thermo Fisher Scientific). .. Total RNA was collected from MSCs using TRI Reagent® (Molecular Research Center Inc., Cincinnati, OH, USA) and reverse-transcribed with the PrimeScript® RT Master Mix Kit (TAKARA Bio Inc., Shiga, Japan).

    Article Title: Treatment of vasculoproliferative conditions with Lrg1 antagonists
    Article Snippet: Isolated and purified microvessel fragments from the retinae of wild type (WT) mice, retina dystrophy (RD) 1 mice, curlytail (CT) mice and very-low density lipoprotein receptor (VLDLR) knock-out mice at time points corresponding to stages when vascular anomalies were present were investigated. .. Microarray gene expression analysis (Affymetrix®) on isolated RNA from the three mouse models revealed 63 genes common to all that were either up- or down-regulated in the microvessels from diseased retina (FIG. 10). ..

    Gene Expression:

    Article Title: Comprehensive genomic profiling of EWSR1/FUS::CREB translocation-associated tumors uncovers prognostically significant recurrent genetic alterations and methylation-transcriptional correlates.
    Article Snippet: .. Affymetrix microarray gene expression analysis Details of the microarray protocol were described previously5,6. .. RNA was isolated using RNAwiz RNA isolation reagent (Ambion) and run through a column with RNase-free DNase (Qiagen).

    Article Title: Diacylglycerol kinase delta overexpression improves glucose clearance and protects against the development of obesity.
    Article Snippet: .. A microarray gene expression analysis was performed by Affymetrix Mouse Gene 1.1 ST array (ThermoFisher Scientific) on the total RNA extracted from EDL muscle wild-type sedentary (WT; n=5), MLC_DGKδ2 transgenic sedentary mice (TG; n=6), wild-type exercised (WT exercised; n=5) and MLC_DGKδ2 transgenic exercised mice (TG exercised; n=5). ..

    Article Title: SLC45A2: A Melanoma Antigen with High Tumor Selectivity and Reduced Potential for Autoimmune Toxicity
    Article Snippet: .. Three days following transduction, GFP-positive melanocytes were sorted and RNA was prepared for Affymetrix microarray gene expression analysis as described previously ( 22 ). ..

    Article Title: Thyroid Hormone Transporters MCT8 and OATP1C1 Control Skeletal Muscle Regeneration
    Article Snippet: .. To obtain a broad overview of the expression pattern of different TH hormone transporters ( ) during myogenesis, we took advantage of our previously published Affymetrix microarray gene expression analysis ( ). ..

    Article Title: Time-course analysis of microRNA-induced mesenchymal-to-epithelial transition underscores the complexity of the underlying molecular processes.
    Article Snippet: .. Three biologically independent RNA samples were collected at each time point (21 samples total) and subjected to microarray gene expression analysis (Affymetrix, U133 +2) as previously described [15]. .. Initial quality control, summarization and normalization of all 21 CEL files using RMA (Robust Multiarray Average) was performed using the Affymetrix Expression Console (software 1.2.1.20) resulting in expression data for 54,613 probe sets (23,518 annotated genes).

    Article Title: TransCONFIRM: Identification of a genetic signature of response to fulvestrant in advanced hormone receptor positive breast cancer
    Article Snippet: .. The PAM50 intrinsic subtype predictions and RS classifications predictions in this study are considered surrogate classifiers since they are based on Affymetrix microarray gene expression analysis and not the original platforms that were used to establish these classifiers (Microarray data from Agilent human 1Av2 microarrays for the PAM50 and RT-PCR for OncotypeDx RS). .. Immunohistochemistry Studies Samples were centrally stained for ER, PR (ER/PR pharmDxTM Kit, Dako), HER2 (HercepTestTM Kit, Dako) and Ki67 (clone MIB-1, dilution 1:100, DAKO).

    Article Title: Transforming Growth Factor β‐Activated Kinase 1 Regulates Mesenchymal Stem Cell Proliferation Through Stabilization of Yap1/Taz Proteins
    Article Snippet: .. The microarray gene expression analysis of DMSO treated BMMSCs and 5zox treated BMMSCs were performed using the Affymetrix ClariomTM S, mouse (Thermo Fisher Scientific) and the Affymetrix Transcriptome Analysis Console software (Thermo Fisher Scientific). .. Total RNA was collected from MSCs using TRI Reagent® (Molecular Research Center Inc., Cincinnati, OH, USA) and reverse-transcribed with the PrimeScript® RT Master Mix Kit (TAKARA Bio Inc., Shiga, Japan).

    Article Title: Treatment of vasculoproliferative conditions with Lrg1 antagonists
    Article Snippet: Isolated and purified microvessel fragments from the retinae of wild type (WT) mice, retina dystrophy (RD) 1 mice, curlytail (CT) mice and very-low density lipoprotein receptor (VLDLR) knock-out mice at time points corresponding to stages when vascular anomalies were present were investigated. .. Microarray gene expression analysis (Affymetrix®) on isolated RNA from the three mouse models revealed 63 genes common to all that were either up- or down-regulated in the microvessels from diseased retina (FIG. 10). ..

    Transgenic Assay:

    Article Title: Diacylglycerol kinase delta overexpression improves glucose clearance and protects against the development of obesity.
    Article Snippet: .. A microarray gene expression analysis was performed by Affymetrix Mouse Gene 1.1 ST array (ThermoFisher Scientific) on the total RNA extracted from EDL muscle wild-type sedentary (WT; n=5), MLC_DGKδ2 transgenic sedentary mice (TG; n=6), wild-type exercised (WT exercised; n=5) and MLC_DGKδ2 transgenic exercised mice (TG exercised; n=5). ..

    Transduction:

    Article Title: SLC45A2: A Melanoma Antigen with High Tumor Selectivity and Reduced Potential for Autoimmune Toxicity
    Article Snippet: .. Three days following transduction, GFP-positive melanocytes were sorted and RNA was prepared for Affymetrix microarray gene expression analysis as described previously ( 22 ). ..

    Expressing:

    Article Title: Thyroid Hormone Transporters MCT8 and OATP1C1 Control Skeletal Muscle Regeneration
    Article Snippet: .. To obtain a broad overview of the expression pattern of different TH hormone transporters ( ) during myogenesis, we took advantage of our previously published Affymetrix microarray gene expression analysis ( ). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: TransCONFIRM: Identification of a genetic signature of response to fulvestrant in advanced hormone receptor positive breast cancer
    Article Snippet: .. The PAM50 intrinsic subtype predictions and RS classifications predictions in this study are considered surrogate classifiers since they are based on Affymetrix microarray gene expression analysis and not the original platforms that were used to establish these classifiers (Microarray data from Agilent human 1Av2 microarrays for the PAM50 and RT-PCR for OncotypeDx RS). .. Immunohistochemistry Studies Samples were centrally stained for ER, PR (ER/PR pharmDxTM Kit, Dako), HER2 (HercepTestTM Kit, Dako) and Ki67 (clone MIB-1, dilution 1:100, DAKO).

    Software:

    Article Title: Transforming Growth Factor β‐Activated Kinase 1 Regulates Mesenchymal Stem Cell Proliferation Through Stabilization of Yap1/Taz Proteins
    Article Snippet: .. The microarray gene expression analysis of DMSO treated BMMSCs and 5zox treated BMMSCs were performed using the Affymetrix ClariomTM S, mouse (Thermo Fisher Scientific) and the Affymetrix Transcriptome Analysis Console software (Thermo Fisher Scientific). .. Total RNA was collected from MSCs using TRI Reagent® (Molecular Research Center Inc., Cincinnati, OH, USA) and reverse-transcribed with the PrimeScript® RT Master Mix Kit (TAKARA Bio Inc., Shiga, Japan).

    Isolation:

    Article Title: Treatment of vasculoproliferative conditions with Lrg1 antagonists
    Article Snippet: Isolated and purified microvessel fragments from the retinae of wild type (WT) mice, retina dystrophy (RD) 1 mice, curlytail (CT) mice and very-low density lipoprotein receptor (VLDLR) knock-out mice at time points corresponding to stages when vascular anomalies were present were investigated. .. Microarray gene expression analysis (Affymetrix®) on isolated RNA from the three mouse models revealed 63 genes common to all that were either up- or down-regulated in the microvessels from diseased retina (FIG. 10). ..



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    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by <t>microarray</t> analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.
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    Agilent technologies two-color microarray-based gene expression analysis microarrays
    Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by <t>microarray.</t> There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.
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    Image Search Results


    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Journal: Journal of Experimental Botany

    Article Title: NAC61 regulates late- and post-ripening osmotic, oxidative, and biotic stress responses in grapevine

    doi: 10.1093/jxb/erad507

    Figure Lengend Snippet: NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Article Snippet: The cDNA synthesis, labelling, hybridization, and washing steps were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide ( https://www.agilent.com/cs/library/usermanuals/Public/G4140-90040_GeneExpression_OneColor_6.9.pdf ).

    Techniques: Expressing, Microarray

    Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by microarray. There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.

    Journal: Frontiers in Oncology

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro

    doi: 10.3389/fonc.2024.1323422

    Figure Lengend Snippet: Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by microarray. There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.

    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Techniques: Microarray, Activation Assay