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Corning Life Sciences microarray hybridization chambers
Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
Microarray Hybridization Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+chamber/microarray+hybridization+chambers/pmc03733744-47-7-10
Average 90 stars, based on 1 article reviews
microarray hybridization chambers - by Bioz Stars, 2026-09
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1) Product Images from "Overexpression of primary microRNA 221/222 in acute myeloid leukemia"

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

Journal: BMC Cancer

doi: 10.1186/1471-2407-13-364

Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
Figure Legend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Techniques Used: Microarray, Quantitative RT-PCR, Expressing

Related Articles

Hybridization:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: Methods and articles for strand-specific polynucleotide detection with conjugated polymers
Article Snippet: .. To demonstrate the integration of the PFBT multichromophore on a substrate comprised of multiple human genome sensor polynucleotides, a microarray slide was prepared in the following manner and tested on labeled target cDNA: Target Hybridization: Target hybridization was performed on a MWG Human Starter Array slide with a LifterSlip (22×22 mm) in a Corning hybridization chamber using Alexa 647 labeled human cDNA. ..

Article Title: o-p'-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and Sprague-Dawley rats.
Article Snippet: Contents lists available at ScienceDirect Toxicology and Applied Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /ytaap o-p′-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and Sprague–Dawley rats Joshua C. Kwekel a,b, Agnes L. Forgacs a,b, Kurt J. Williams c, Timothy R. Zacharewski a,b,⁎ a Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI, USA b Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA c Pathobiology & Diagnostic Investigation, Michigan State University, East Lansing, MI, USA ⁎ Corresponding author at: 603 Wilson Road, Bioc Michigan State University, East Lansing, MI 48824, USA.. F E-mail address: tzachare@msu.edu (T.R.. Zacharewski) 0041-008X/$ – see front matter © 2013 Elsevier Inc. All ri http://dx.doi.org/10.1016/j.taap.2013.09.024 a b s t r a c t a r t i c l e i n f o

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Microarray:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: Methods and articles for strand-specific polynucleotide detection with conjugated polymers
Article Snippet: .. To demonstrate the integration of the PFBT multichromophore on a substrate comprised of multiple human genome sensor polynucleotides, a microarray slide was prepared in the following manner and tested on labeled target cDNA: Target Hybridization: Target hybridization was performed on a MWG Human Starter Array slide with a LifterSlip (22×22 mm) in a Corning hybridization chamber using Alexa 647 labeled human cDNA. ..

Article Title: Aptamer microarray as a novel bioassay for protein-protein interaction discovery and analysis.
Article Snippet: Aptamer microarray is investigated as a novel bioassay for protein–protein interaction (PPI) discovery and analysis.. Assaying a mixture of fluorescence-labeled thrombin and Escherichia coli proteins with an aptamer microarray, we found that thrombin and an unknown protein of E. coli (protein X) formed a complex of PPI, which was captured by an anti-thrombin aptamer probe.. The PPI observed on the microarray was double-checked by protein microarrays and confirmed by aptamer-baited co-immunoprecipitation (Co-IP) assays.

Article Title: o-p'-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and Sprague-Dawley rats.
Article Snippet: Contents lists available at ScienceDirect Toxicology and Applied Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /ytaap o-p′-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and Sprague–Dawley rats Joshua C. Kwekel a,b, Agnes L. Forgacs a,b, Kurt J. Williams c, Timothy R. Zacharewski a,b,⁎ a Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI, USA b Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA c Pathobiology & Diagnostic Investigation, Michigan State University, East Lansing, MI, USA ⁎ Corresponding author at: 603 Wilson Road, Bioc Michigan State University, East Lansing, MI 48824, USA.. F E-mail address: tzachare@msu.edu (T.R.. Zacharewski) 0041-008X/$ – see front matter © 2013 Elsevier Inc. All ri http://dx.doi.org/10.1016/j.taap.2013.09.024 a b s t r a c t a r t i c l e i n f o

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

other:

Article Title: Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.
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Labeling:

Article Title: Methods and articles for strand-specific polynucleotide detection with conjugated polymers
Article Snippet: .. To demonstrate the integration of the PFBT multichromophore on a substrate comprised of multiple human genome sensor polynucleotides, a microarray slide was prepared in the following manner and tested on labeled target cDNA: Target Hybridization: Target hybridization was performed on a MWG Human Starter Array slide with a LifterSlip (22×22 mm) in a Corning hybridization chamber using Alexa 647 labeled human cDNA. ..

Centrifugation:

Article Title: o-p'-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and Sprague-Dawley rats.
Article Snippet: Contents lists available at ScienceDirect Toxicology and Applied Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /ytaap o-p′-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and Sprague–Dawley rats Joshua C. Kwekel a,b, Agnes L. Forgacs a,b, Kurt J. Williams c, Timothy R. Zacharewski a,b,⁎ a Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI, USA b Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA c Pathobiology & Diagnostic Investigation, Michigan State University, East Lansing, MI, USA ⁎ Corresponding author at: 603 Wilson Road, Bioc Michigan State University, East Lansing, MI 48824, USA.. F E-mail address: tzachare@msu.edu (T.R.. Zacharewski) 0041-008X/$ – see front matter © 2013 Elsevier Inc. All ri http://dx.doi.org/10.1016/j.taap.2013.09.024 a b s t r a c t a r t i c l e i n f o

Incubation:

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..



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Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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https://www.bioz.com/product/microarray+chamber/microarray+hybridization+chambers/pmc03733744-47-7-10
Average 90 stars, based on 1 article reviews
microarray hybridization chambers - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Journal: BMC Cancer

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

doi: 10.1186/1471-2407-13-364

Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

Techniques: Microarray, Quantitative RT-PCR, Expressing