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Camlab Ltd microarray hybridization chamber
Microarray Hybridization Chamber, supplied by Camlab Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+chamber/hybridization+chamber/pm40561749-99-7-10
Average 90 stars, based on 1 article reviews
microarray hybridization chamber - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Improved serum biomarker detection via self-clickable Cu 2 O nanoparticle-coated Ag slides with oriented and stable antibody presentation on antifouling surfaces.
Article Snippet: We propose an innovative approach to immobilize antibodies (Abs) on solid supports, addressing challenges in generating immunosensor arrays.. This method involves a self-clickable Cu2O-nanoparticle-coated silver (Cu2O@Ag) surface for boronic acid (BA) functionalization, facilitating the irreversible immobilization of native Abs.. The surface catalyzes azide-alkyne cycloaddition with azido-containing BA molecules without requiring an external Cu(I) ion source.

Article Title: Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
Article Snippet: After a DNaseI treatment, the probe was labeled by nick translation with Cy3-dCTP (Amersham) according to the manufacturer's protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 μl of hybridization buffer (50% formamide, 10% dextran sulfate, 4× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts
Article Snippet: After DNase I treatment, the probe was labeled by nick translation using Cy3-dCTP (PA 53021; Amersham) according to the manufacturer's protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 μl hybridization buffer (50% formamide, 10% dextran sulfate, 4× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
Article Snippet: After a DNaseI treatment, the probe was labeled by nick translation with Cy3-dCTP (Amersham) according to the manufacturer’s protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 l of hybridization buffer (50% formamide, 10% dextran sulfate, 4 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts
Article Snippet: After DNase I treatment, the probe was labeled by nick translation using Cy3-dCTP (PA 53021; Amersham) according to the manufacturer’s protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 l hybridization buffer (50% formamide, 10% dextran sulfate, 4 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Regioselective S N 2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation.
Article Snippet: Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications.. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation Page 1 of 40 ACS Paragon Plus Environment ACS Applied Bio Materials 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Microarray:

Article Title: Improved serum biomarker detection via self-clickable Cu 2 O nanoparticle-coated Ag slides with oriented and stable antibody presentation on antifouling surfaces.
Article Snippet: We propose an innovative approach to immobilize antibodies (Abs) on solid supports, addressing challenges in generating immunosensor arrays.. This method involves a self-clickable Cu2O-nanoparticle-coated silver (Cu2O@Ag) surface for boronic acid (BA) functionalization, facilitating the irreversible immobilization of native Abs.. The surface catalyzes azide-alkyne cycloaddition with azido-containing BA molecules without requiring an external Cu(I) ion source.

Article Title: Small Molecule Microarrays
Article Snippet: .. Microarray hybrid chamber (CamLab, UK). ..

Article Title: Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
Article Snippet: After a DNaseI treatment, the probe was labeled by nick translation with Cy3-dCTP (Amersham) according to the manufacturer's protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 μl of hybridization buffer (50% formamide, 10% dextran sulfate, 4× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts
Article Snippet: After DNase I treatment, the probe was labeled by nick translation using Cy3-dCTP (PA 53021; Amersham) according to the manufacturer's protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 μl hybridization buffer (50% formamide, 10% dextran sulfate, 4× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
Article Snippet: After a DNaseI treatment, the probe was labeled by nick translation with Cy3-dCTP (Amersham) according to the manufacturer’s protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 l of hybridization buffer (50% formamide, 10% dextran sulfate, 4 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts
Article Snippet: After DNase I treatment, the probe was labeled by nick translation using Cy3-dCTP (PA 53021; Amersham) according to the manufacturer’s protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 l hybridization buffer (50% formamide, 10% dextran sulfate, 4 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Locus-Specific Gene Expression Pattern Suggests a Unique Propagation Strategy for a Giant Algal Virus
Article Snippet: For hybridization to microarrays, 7.5 l 20 SSC (Sigma), 1.0 l 10% SDS, and the labeled cRNA sample were combined in a total volume of 50 l. After being incubated at 100°C for 2 min (lid temperature, 110°C), the samples were cooled (room temperature, 5 min) and the volume was checked (and made up to 50 l when necessary) prior to loading the samples onto the microarray slide covered by a LifterSlip (Erie Scientific Company). .. Microarray hybridization was performed in a microarray hybrid chamber (Camlab) at 65°C for 18 h. The microarray slides were given three posthybridization washes. ..

Article Title: Regioselective S N 2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation.
Article Snippet: Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications.. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation Page 1 of 40 ACS Paragon Plus Environment ACS Applied Bio Materials 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Hybridization:

Article Title: Improved serum biomarker detection via self-clickable Cu 2 O nanoparticle-coated Ag slides with oriented and stable antibody presentation on antifouling surfaces.
Article Snippet: We propose an innovative approach to immobilize antibodies (Abs) on solid supports, addressing challenges in generating immunosensor arrays.. This method involves a self-clickable Cu2O-nanoparticle-coated silver (Cu2O@Ag) surface for boronic acid (BA) functionalization, facilitating the irreversible immobilization of native Abs.. The surface catalyzes azide-alkyne cycloaddition with azido-containing BA molecules without requiring an external Cu(I) ion source.

Article Title: Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
Article Snippet: After a DNaseI treatment, the probe was labeled by nick translation with Cy3-dCTP (Amersham) according to the manufacturer's protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 μl of hybridization buffer (50% formamide, 10% dextran sulfate, 4× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts
Article Snippet: After DNase I treatment, the probe was labeled by nick translation using Cy3-dCTP (PA 53021; Amersham) according to the manufacturer's protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 μl hybridization buffer (50% formamide, 10% dextran sulfate, 4× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
Article Snippet: After a DNaseI treatment, the probe was labeled by nick translation with Cy3-dCTP (Amersham) according to the manufacturer’s protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 l of hybridization buffer (50% formamide, 10% dextran sulfate, 4 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts
Article Snippet: After DNase I treatment, the probe was labeled by nick translation using Cy3-dCTP (PA 53021; Amersham) according to the manufacturer’s protocol. .. The cells were prehybridized by incubation for 30 min at 37°C with 20 l hybridization buffer (50% formamide, 10% dextran sulfate, 4 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate]) in a humidified microarray hybridization chamber (Camlab). ..

Article Title: Locus-Specific Gene Expression Pattern Suggests a Unique Propagation Strategy for a Giant Algal Virus
Article Snippet: For hybridization to microarrays, 7.5 l 20 SSC (Sigma), 1.0 l 10% SDS, and the labeled cRNA sample were combined in a total volume of 50 l. After being incubated at 100°C for 2 min (lid temperature, 110°C), the samples were cooled (room temperature, 5 min) and the volume was checked (and made up to 50 l when necessary) prior to loading the samples onto the microarray slide covered by a LifterSlip (Erie Scientific Company). .. Microarray hybridization was performed in a microarray hybrid chamber (Camlab) at 65°C for 18 h. The microarray slides were given three posthybridization washes. ..

Article Title: Regioselective S N 2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation.
Article Snippet: Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications.. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation Page 1 of 40 ACS Paragon Plus Environment ACS Applied Bio Materials 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Saline:

Article Title: Improved serum biomarker detection via self-clickable Cu 2 O nanoparticle-coated Ag slides with oriented and stable antibody presentation on antifouling surfaces.
Article Snippet: We propose an innovative approach to immobilize antibodies (Abs) on solid supports, addressing challenges in generating immunosensor arrays.. This method involves a self-clickable Cu2O-nanoparticle-coated silver (Cu2O@Ag) surface for boronic acid (BA) functionalization, facilitating the irreversible immobilization of native Abs.. The surface catalyzes azide-alkyne cycloaddition with azido-containing BA molecules without requiring an external Cu(I) ion source.

Article Title: Regioselective S N 2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation.
Article Snippet: Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications.. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation Page 1 of 40 ACS Paragon Plus Environment ACS Applied Bio Materials 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Gentle:

Article Title: Regioselective S N 2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation.
Article Snippet: Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications.. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation Page 1 of 40 ACS Paragon Plus Environment ACS Applied Bio Materials 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60



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Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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Image Search Results


Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Journal: BMC Cancer

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

doi: 10.1186/1471-2407-13-364

Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

Techniques: Microarray, Quantitative RT-PCR, Expressing