Journal: Nature Communications

Article Title: Recycling senescent cell lipids for targeted senotherapy

doi: 10.1038/s41467-026-70486-0

Figure Lengend Snippet: a Schematic comparing targeted senorecycle therapy vs. senolytics: targeting, non-apoptotic lipid extraction, sustained efficacy, dual chondroprotection-lubrication. b Experimental workflow for comparative analysis: Following mild senescence (IL-1β, 5 ng/mL,8 h, Bleomycin, 5 μg/mL, 8 h) induction, cells were treated with either D + Q (Dasatinib, 200 nM plus Quercetin, 10 μm) or MβCD@ICAM1-NPs for 24 h (MβCD 50 μg/mL), followed by parallel qPCR analysis of related genes and ELISA quantification of secreted proteins. c ELISA results of inflammatory mediators (IL-6, Mmp9, Mmp13, Ccl2) and DAMPs (Hmgb1) in conditioned media from senolytic- or NP-treated senescence (IL-1β) group. n = 8 biologically independent cell cultures (mean ± s.d.). d Workflow for detecting the effects of the two therapies on normal proliferating primary chondrocytes for 4 days: qPCR analysis of collected cells and ELISA quantification of secreted proteins in supernatants. e Trends of secretion levels of IL-6, Mmp9, Mmp13 and Ccl2 in the supernatant across two therapies ( n = 4, mean ± s.d.). f Trends of qPCR results of IL-6 , Mmp13 , Col2a1 and Aggrecan in cells across two treatments ( n = 3, mean ± s.d.). g Flow chart of the friction coefficient detection under different treatments. Fresh control or OA implants from the same part were collected, and the overall friction coefficient was detected after incubated with senolytics (D + Q) or MINH for 24 h. h COF-time curves (1200 s) across treatment groups. i Schematic diagram of in vivo therapeutic comparison using p16-3MR mice. j Quantitative analysis of 50% paw withdraw threshold across groups. n = 8 mice per group (mean ± s.d.). k Visualization results of the medial subchondral bone plate in each group(top) and the 3D reconstruction of murine knee joints (bottom). The heatmap shows its bone density distribution. Yellow arrows indicate osteophyte formation. l Representative images of Safranin-O staining results of knee joint sections across groups. The same position (black dashed box) in the above figure is enlarged and shown below (scale bar, 50 μm). m Representative images of immunohistochemical staining (Aggrecan, Col2a1, IL-6 and Mmp13) of medial tibia sections of knee joints across groups (scale bar, 50 μm). P values were determined using one-way ANOVA with a Tukey post-hoc test ( c , j ). Source data are provided as a Source Data file.

Article Snippet: MβCD (MCE, HY-101461, 50 μg/mL), Dasatinib (MCE, HY-10181, 200 nM), Quercetin (MCE, HY-18085, 10 μM).

Techniques: Extraction, Enzyme-linked Immunosorbent Assay, Control, Incubation, In Vivo, Comparison, Staining, Immunohistochemical staining

Journal: Nature Communications

Article Title: Recycling senescent cell lipids for targeted senotherapy

doi: 10.1038/s41467-026-70486-0

Figure Lengend Snippet: a Schematic comparing targeted senorecycle therapy vs. senolytics: targeting, non-apoptotic lipid extraction, sustained efficacy, dual chondroprotection-lubrication. b Experimental workflow for comparative analysis: Following mild senescence (IL-1β, 5 ng/mL,8 h, Bleomycin, 5 μg/mL, 8 h) induction, cells were treated with either D + Q (Dasatinib, 200 nM plus Quercetin, 10 μm) or MβCD@ICAM1-NPs for 24 h (MβCD 50 μg/mL), followed by parallel qPCR analysis of related genes and ELISA quantification of secreted proteins. c ELISA results of inflammatory mediators (IL-6, Mmp9, Mmp13, Ccl2) and DAMPs (Hmgb1) in conditioned media from senolytic- or NP-treated senescence (IL-1β) group. n = 8 biologically independent cell cultures (mean ± s.d.). d Workflow for detecting the effects of the two therapies on normal proliferating primary chondrocytes for 4 days: qPCR analysis of collected cells and ELISA quantification of secreted proteins in supernatants. e Trends of secretion levels of IL-6, Mmp9, Mmp13 and Ccl2 in the supernatant across two therapies ( n = 4, mean ± s.d.). f Trends of qPCR results of IL-6 , Mmp13 , Col2a1 and Aggrecan in cells across two treatments ( n = 3, mean ± s.d.). g Flow chart of the friction coefficient detection under different treatments. Fresh control or OA implants from the same part were collected, and the overall friction coefficient was detected after incubated with senolytics (D + Q) or MINH for 24 h. h COF-time curves (1200 s) across treatment groups. i Schematic diagram of in vivo therapeutic comparison using p16-3MR mice. j Quantitative analysis of 50% paw withdraw threshold across groups. n = 8 mice per group (mean ± s.d.). k Visualization results of the medial subchondral bone plate in each group(top) and the 3D reconstruction of murine knee joints (bottom). The heatmap shows its bone density distribution. Yellow arrows indicate osteophyte formation. l Representative images of Safranin-O staining results of knee joint sections across groups. The same position (black dashed box) in the above figure is enlarged and shown below (scale bar, 50 μm). m Representative images of immunohistochemical staining (Aggrecan, Col2a1, IL-6 and Mmp13) of medial tibia sections of knee joints across groups (scale bar, 50 μm). P values were determined using one-way ANOVA with a Tukey post-hoc test ( c , j ). Source data are provided as a Source Data file.

Article Snippet: MβCD (MCE, HY-101461, 50 μg/mL), Dasatinib (MCE, HY-10181, 200 nM), Quercetin (MCE, HY-18085, 10 μM).

Techniques: Extraction, Enzyme-linked Immunosorbent Assay, Control, Incubation, In Vivo, Comparison, Staining, Immunohistochemical staining