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methemoglobin (methb  (Millipore)


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    Millipore methemoglobin (methb
    Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, <t>methemoglobin;</t> HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.
    Methemoglobin (Methb, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methb/methemoglobin++methb/pmc11208336-67-2-4
    Average 90 stars, based on 1 article reviews
    methemoglobin (methb - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Porphyromonas endodontalis HmuY differentially participates in heme acquisition compared to the Porphyromonas gingivalis and Tannerella forsythia hemophore-like proteins"

    Article Title: Porphyromonas endodontalis HmuY differentially participates in heme acquisition compared to the Porphyromonas gingivalis and Tannerella forsythia hemophore-like proteins

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2024.1421018

    Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, methemoglobin; HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.
    Figure Legend Snippet: Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, methemoglobin; HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.

    Techniques Used: Comparison, Activity Assay, Produced, Purification, Concentration Assay, Incubation, SDS Page, Staining

    Related Articles

    other:

    Article Title: Fluorescence depth estimation from wide-field optical imaging data for guiding brain tumor resection: a multi-inclusion phantom study
    Article Snippet: For the bulk media, concentrations of metHb (Sigma Aldrich) and Intralipid (Intralipid 20%, Patterson Veterinary Supply) were diluted to produce absorption coefficient and reduced scattering coefficient values that span the range of optical properties found in brain [ 17 , 18 ].

    Size-exclusion Chromatography:

    Article Title: Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA: the human hemoglobin receptor in Staphylococcus aureus.
    Article Snippet: 0022-2836/$ see front matter © 2006 E During infections the pathogen Staphylococcus aureus procures the essential nutrient iron from its host using iron-regulated surface determinant (Isd) proteins, which scavenge heme bound iron from host hemoproteins.. Four Isd proteins are displayed in the cell wall, where they function as receptors for host proteins and heme.. Each of the receptors contains one or more copies of a recently discovered domain called NEAT (NEAr Transporter) that has been shown to mediate protein binding.



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    Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, <t>methemoglobin;</t> HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.
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    Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, <t>methemoglobin;</t> HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.
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    Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, <t>methemoglobin;</t> HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.
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    Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, methemoglobin; HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Porphyromonas endodontalis HmuY differentially participates in heme acquisition compared to the Porphyromonas gingivalis and Tannerella forsythia hemophore-like proteins

    doi: 10.3389/fcimb.2024.1421018

    Figure Lengend Snippet: Comparison of the proteolytic activity of P. endodontalis and P. gingivalis . (A) Total proteolytic activity in whole bacterial cultures (iron- and heme-rich medium; Hm medium) was determined using azocasein as a substrate. The activity is shown as an increase in absorbance at 450 nm (A 450 ) caused by the release of a colored product within 60 minutes by 1 ml of bacterial culture with an optical density at 600 nm of 1. (B) Susceptibility of human hemoproteins and selected HmuY proteins to degradation by proteases produced by P. endodontalis or P. gingivalis . Purified proteins were added to the bacterial cultures (Hm medium) at a final 2 µM concentration, samples were incubated, collected at the indicated time points, and analyzed by SDS-PAGE and CBB G-250 staining. As a control, Hm medium alone was used instead of bacterial cultures. (C) Protein pattern of P. gingivalis or P. endodontalis proteins in iron- and heme-rich culture medium (Hm medium) without adding host hemoproteins or HmuY proteins. metHb, methemoglobin; HSA, serum albumin; Hpx, hemopexin; HmuY Pg , P. gingivalis HmuY; HmuY Pe , P. endodontalis HmuY homolog; HmuY Tf , T. forsythia HmuY homolog; M, protein molecular mass markers. **** P <0.0001.

    Article Snippet: Except for methemoglobin (metHb; Sigma-Aldrich), protein-heme complexes were prepared by mixing protein and heme at a 1:1.2 molar ratio and incubation at room temperature for 1 hour.

    Techniques: Comparison, Activity Assay, Produced, Purification, Concentration Assay, Incubation, SDS Page, Staining