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mouse embryonic stem cells cell mes lines d3  (ATCC)


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    ATCC mouse embryonic stem cells cell mes lines d3
    Mouse Embryonic Stem Cells Cell Mes Lines D3, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mes+d3+cells/ES-D3%3B+Embryonic+Stem+Cell%3B+Mouse/pm36936789-218-18-26
    Average 91 stars, based on 1 article reviews
    mouse embryonic stem cells cell mes lines d3 - by Bioz Stars, 2026-09
    91/100 stars

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    Expressing:

    Article Title: Cell-based screening of traditional Chinese medicines for proliferation enhancers of mouse embryonic stem cells.
    Article Snippet: A high-throughput cell-based method was developed for screening traditional Chinese herbal medicines (TCHMs) for potential stem cell growth promoters.. Mouse embryonic stem (mES) cells expressing enhanced green fluorescent protein (EGFP) were cultured in growth media supplemented with various TCHM extracts.. The dosage-dependent effects of TCHM extracts on cell growth, including proliferation and cytotoxicity, were assessed via EGFP fluorescence measurement.

    Cell Culture:

    Article Title: Embryotoxicity estimation of commonly used compounds with embryonic stem cell test
    Article Snippet: .. mES-D3 cells (CRL1934; ATCC, Manassas, VA, USA) and BALB/c 3T3 cells (CCL-163; ATCC) were cultured at 37°C in a 5% CO 2 atmosphere. mES cells were routinely cultured on mouse embryonic fibroblast feeder (0303–200; Innovative Cellular Therapeutics, Co., Ltd., Shanghai, China) in the presence of leukemia inhibitory factor (LIF; PMC9484; 1,000 U/ml, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) to maintain their undifferentiated status and were passaged every second day. mES cell medium consisted of Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM GlutaMAX-I Supplement, 1% non-essential amino acids (all Gibco; Thermo Fisher Scientific, Inc.), 0.1% β-mercaptoethanol (Merck KGaA, Darmstadt, Germany), 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich; Merck KGaA). .. Maintenance of BALB/c 3T3 cells used products from Gibco; Thermo Fisher Scientific, Inc., unless otherwise stated.

    Modification:

    Article Title: Embryotoxicity estimation of commonly used compounds with embryonic stem cell test
    Article Snippet: .. mES-D3 cells (CRL1934; ATCC, Manassas, VA, USA) and BALB/c 3T3 cells (CCL-163; ATCC) were cultured at 37°C in a 5% CO 2 atmosphere. mES cells were routinely cultured on mouse embryonic fibroblast feeder (0303–200; Innovative Cellular Therapeutics, Co., Ltd., Shanghai, China) in the presence of leukemia inhibitory factor (LIF; PMC9484; 1,000 U/ml, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) to maintain their undifferentiated status and were passaged every second day. mES cell medium consisted of Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM GlutaMAX-I Supplement, 1% non-essential amino acids (all Gibco; Thermo Fisher Scientific, Inc.), 0.1% β-mercaptoethanol (Merck KGaA, Darmstadt, Germany), 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich; Merck KGaA). .. Maintenance of BALB/c 3T3 cells used products from Gibco; Thermo Fisher Scientific, Inc., unless otherwise stated.



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    RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in <t>undifferentiated</t> <t>mES</t> cells and neuron-like cells differentiated from mES cells. <t>D3:</t> Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).
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    RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in undifferentiated mES cells and neuron-like cells differentiated from mES cells. D3: Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).

    Journal: Developmental dynamics : an official publication of the American Association of Anatomists

    Article Title: Concomitant differentiation of a population of mouse embryonic stem cells into neuron-like cells and Schwann cell-like cells in a slow-flow microfluidic device

    doi: 10.1002/dvdy.24466

    Figure Lengend Snippet: RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in undifferentiated mES cells and neuron-like cells differentiated from mES cells. D3: Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).

    Article Snippet: Culturing mES cells Cell lines: The cell line used for these studies was the D3 mES cell line (ATCC-CRL-1934; Doetschman et al., 1985 ).

    Techniques: Marker, Expressing, Control