memerald sec61β (Addgene inc)

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Addgene inc memerald sec61β
Memerald Sec61β, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/memerald+sec61b/mEmerald-Sec61b-C1+(Plasmid+%2390992)/10__1038_slash_s41467___026___78424___w-464-2-3
Average 93 stars, based on 30 article reviews

memerald sec61β - by Bioz Stars, 2026-10

93/100 stars

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Article Title: Periodic ER-plasma membrane junctions support long-range Ca 2+ signal integration in dendrites.
Article Snippet: .. pAAV hSyn INTRON mEmerald-Sec61b WPRE, pAAV hSyn INTRON HaloTag-Sec61b WPRE, pAAV hSyn INTRON mEmerald-ER WPRE, pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE, pAAV hSyn INTRON mScarlet-CAAX2 WPRE, pAAV hSyn INTRON HaloTag-STIM1 were generated subcloning the cORF of mEmerald-Sec61b (from Addgene #90992), HaloTag-Sec61b (Addgene #123285), mEmerald-ER3 (Addgene #54082), mTagBFP2-CAAX2 (synthesized with gBlock), mScarletI-CAAX2 (synthesized with gBlock), HaloTag-STIM1 (synthesized with gBlock) at BamHI and HindIII sites of pAAV Syn intron Psam4GlyR m3m4 HA (a gift from Scott Sternson, Addgene #196041). pAAV hSyn INTRON mEmerald-STIM1 WPRE was generated subcloning SS-mEmeraldlinker (synthesized with gBlock) at BamHI and BsrGI restriction enzyme sites of pAAV hSyn INTRON HaloTag-STIM1 WPRE. .. STIM2 construct design was based on SP-YFP-STM2(15-746) (Addgene #18862).

Subcloning:

Synthesized:

Plasmid Preparation:

Article Title: The endoplasmic reticulum adopts two distinct tubule forms
Article Snippet: .. Rtn4a-GFP (Addgene plasmid #61807) and mCherry-Climp63(mouse) (Addgene plasmid #136293) were gifts from Gia Voeltz . mEmerald-sec61b (Addgene plasmid #54249), mEmerald-ER-3 (Addgene plasmid #54082), and calnexin-mEmerlad (Addgene plasmid #54021) were gifts from Michael Davidson. pcDNA3.1(+) backbone was prepared by digesting the pcDNA3.1(+) IRES GFP plasmid (Addgene plasmid #51406; a gift from Kathleen Collins) using the restriction enzymes EcoRI (ThermoFisher, FD0274) and XbaI (ThermoFisher, FD0684). .. Rtn4b-GFP was generated by inserting two PCR-amplified fragments from the initial part (RTN4 AA1-AA185) and the later part (RTN4 AA1005-AA1192 plus AcGFP1) of Rtn4a-GFP into the pcDNA3.1(+) backbone using Gibson Assembly (New England Biolabs, #E2611S).

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