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membrane based antibody array  (R&D Systems)


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    Structured Review

    R&D Systems membrane based antibody array
    Membrane Based Antibody Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+based+antibody+array/Proteome+Profiler+Mouse+XL+Cytokine+Array/pm40706974-106-1-10
    Average 96 stars, based on 522 article reviews
    membrane based antibody array - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Chemotherapy induced cognitive impairment is associated with increased inflammation and oxidative damage in the hippocampus
    Article Snippet: In the present study, the relative levels of various cytokines and chemokines were determined simultaneously using a membrane based antibody array (R&D, ARY008). .. In the present study, the relative levels of various cytokines and chemokines were determined simultaneously using a membrane based antibody array (R&D, ARY008). ..

    Article Title: Constitutive secretion of pro-IL-18 allows keratinocytes to initiate inflammation during bacterial infection
    Article Snippet: For cell lysis, 0.05% triton X-100 (Sigma) was added 5 min. Other cell treatments are 5 μM caspase-1 inhibitor YVAD-fmk (R&D Systems), 50 μM SpeB inhibitor E-64 (Sigma), 20 μg/mL anti-IL-18 IgG (Abcam), or 10 μM caspase-1 inhibitor VX-765 (Invivogen), for the full duration of the experiment. .. The relative levels of selected human cytokines and chemokines were determined in parallel using a membrane-based antibody array (ARY005B, R&D Systems). ..

    Article Title: ADAM8 affects glioblastoma progression by regulating osteopontin-mediated angiogenesis.
    Article Snippet: For the OPN "rescue" assay, 2.5 ng/ml OPN (PeproTech, 120-35) was added to macrophage and 50 ng/ml OPN to U87 supernatants. .. A membrane-based antibody array [Proteome Profiler® Mouse XL Cytokine Array ARY028, RandD Systems, Abingdon, UK] was used to identify cytokine expression levels under different conditions, according to the manufacturer’s protocol. ..

    Article Title: High salt diet intake promotes the induction of experimental autoimmune encephalomyelitis by exacerbating neutrophil infiltration and microglial activation.
    Article Snippet: Although the cause of multiple sclerosis (MS) is not fully known, environmental and lifestyle factors are considered significant risk factors for its development and progression of experimental autoimmune encephalomyelitis (EAE), an animal model of MS.. Here, we found that dietary high salt (HS) intake significantly exacerbated the clinical scores of myelin oligodendrocyte glycoprotein-induced EAE mice in the acute phase, but not in the chronic phase.. During the acute phase of EAE, HS diet intake selectively promoted neutrophil infiltration into the spinal cord without affecting T cell, B cell, and dendritic cell infiltration.

    Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson's disease.
    Article Snippet: .. For the measurement we used a membrane-based antibody array (ARY006, R&D Systems) and followed the provider’s protocol. ..

    Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson’s disease
    Article Snippet: .. For the measurement we used a membrane-based antibody array (ARY006, R&D Systems) and followed the provider’s protocol. ..

    Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson’s disease
    Article Snippet: .. For the measurement we used a membrane-based antibody array (ARY006, R&D Systems) and followed the provider’s protocol. ..

    Ab Array:

    Article Title: Chemotherapy induced cognitive impairment is associated with increased inflammation and oxidative damage in the hippocampus
    Article Snippet: In the present study, the relative levels of various cytokines and chemokines were determined simultaneously using a membrane based antibody array (R&D, ARY008). .. In the present study, the relative levels of various cytokines and chemokines were determined simultaneously using a membrane based antibody array (R&D, ARY008). ..

    Article Title: Constitutive secretion of pro-IL-18 allows keratinocytes to initiate inflammation during bacterial infection
    Article Snippet: For cell lysis, 0.05% triton X-100 (Sigma) was added 5 min. Other cell treatments are 5 μM caspase-1 inhibitor YVAD-fmk (R&D Systems), 50 μM SpeB inhibitor E-64 (Sigma), 20 μg/mL anti-IL-18 IgG (Abcam), or 10 μM caspase-1 inhibitor VX-765 (Invivogen), for the full duration of the experiment. .. The relative levels of selected human cytokines and chemokines were determined in parallel using a membrane-based antibody array (ARY005B, R&D Systems). ..

    Article Title: ADAM8 affects glioblastoma progression by regulating osteopontin-mediated angiogenesis.
    Article Snippet: For the OPN "rescue" assay, 2.5 ng/ml OPN (PeproTech, 120-35) was added to macrophage and 50 ng/ml OPN to U87 supernatants. .. A membrane-based antibody array [Proteome Profiler® Mouse XL Cytokine Array ARY028, RandD Systems, Abingdon, UK] was used to identify cytokine expression levels under different conditions, according to the manufacturer’s protocol. ..

    Article Title: High salt diet intake promotes the induction of experimental autoimmune encephalomyelitis by exacerbating neutrophil infiltration and microglial activation.
    Article Snippet: Although the cause of multiple sclerosis (MS) is not fully known, environmental and lifestyle factors are considered significant risk factors for its development and progression of experimental autoimmune encephalomyelitis (EAE), an animal model of MS.. Here, we found that dietary high salt (HS) intake significantly exacerbated the clinical scores of myelin oligodendrocyte glycoprotein-induced EAE mice in the acute phase, but not in the chronic phase.. During the acute phase of EAE, HS diet intake selectively promoted neutrophil infiltration into the spinal cord without affecting T cell, B cell, and dendritic cell infiltration.

    Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson's disease.
    Article Snippet: .. For the measurement we used a membrane-based antibody array (ARY006, R&D Systems) and followed the provider’s protocol. ..

    Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson’s disease
    Article Snippet: .. For the measurement we used a membrane-based antibody array (ARY006, R&D Systems) and followed the provider’s protocol. ..

    Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson’s disease
    Article Snippet: .. For the measurement we used a membrane-based antibody array (ARY006, R&D Systems) and followed the provider’s protocol. ..

    Expressing:

    Article Title: ADAM8 affects glioblastoma progression by regulating osteopontin-mediated angiogenesis.
    Article Snippet: For the OPN "rescue" assay, 2.5 ng/ml OPN (PeproTech, 120-35) was added to macrophage and 50 ng/ml OPN to U87 supernatants. .. A membrane-based antibody array [Proteome Profiler® Mouse XL Cytokine Array ARY028, RandD Systems, Abingdon, UK] was used to identify cytokine expression levels under different conditions, according to the manufacturer’s protocol. ..



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    RayBiotech inc human cytokine antibody array membrane-based elisa kit
    ( A ) Schematic demonstrating the design of leukemia-on-a-chip that consists of three functional regions. ( B ) Whole scan of the resultant leukemic BM niche system, where the enlarged area shows the colocalization of murine B-ALL cells within the perivascular niche. The bottom insert shows colocalization in the endosteal niche. ( C ) Hematoxylin and eosin (H&E) staining image of the in vivo murine leukemic BM niche, with the enlarged area showing the colocalization of B-ALL cells within the perivascular and endosteal niches. ( D ) The chemoresistance was compared between the engineered human BM niches of ETV6-RUNX1 + REH and Ph + SUP-B15 B-ALL. Each drug concentration had three or more experimental replicates. ( E ) <t>The</t> <t>cytokine</t> profiles from two B-ALL blasts with and without niche cells were quantified using membrane-based enzyme-linked immunosorbent assay <t>(ELISA)</t> analysis. MCP-1, monocyte chemoattractant protein-1; MIG, monokine induced by gamma interferon. ( F ) Quantification of nuclear (Nuc)/cytoplasmic (Cyto) ratio of NF-κB in REH and SUP B-ALL within their respective niche models. The ratios for REH and SUP were manually measured from three experimental replicates ( n > 150). ( G ) Percentage of Ki67 + B-ALL cells, corresponding to (F). Data were collected from three experimental replicates. Unpaired t test (** P < 0.01, Mann-Whitney test). GCSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; GRO, growth-regulated oncogene; IL, interleukin; IFN-γ, interferon-γ; TGFβ1, transforming growth factor–β1; TNFα, tumor necrosis factor–α; DAPI, 4′,6-diamidino-2-phenylindole.
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    Image Search Results


    ( A ) Schematic demonstrating the design of leukemia-on-a-chip that consists of three functional regions. ( B ) Whole scan of the resultant leukemic BM niche system, where the enlarged area shows the colocalization of murine B-ALL cells within the perivascular niche. The bottom insert shows colocalization in the endosteal niche. ( C ) Hematoxylin and eosin (H&E) staining image of the in vivo murine leukemic BM niche, with the enlarged area showing the colocalization of B-ALL cells within the perivascular and endosteal niches. ( D ) The chemoresistance was compared between the engineered human BM niches of ETV6-RUNX1 + REH and Ph + SUP-B15 B-ALL. Each drug concentration had three or more experimental replicates. ( E ) The cytokine profiles from two B-ALL blasts with and without niche cells were quantified using membrane-based enzyme-linked immunosorbent assay (ELISA) analysis. MCP-1, monocyte chemoattractant protein-1; MIG, monokine induced by gamma interferon. ( F ) Quantification of nuclear (Nuc)/cytoplasmic (Cyto) ratio of NF-κB in REH and SUP B-ALL within their respective niche models. The ratios for REH and SUP were manually measured from three experimental replicates ( n > 150). ( G ) Percentage of Ki67 + B-ALL cells, corresponding to (F). Data were collected from three experimental replicates. Unpaired t test (** P < 0.01, Mann-Whitney test). GCSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; GRO, growth-regulated oncogene; IL, interleukin; IFN-γ, interferon-γ; TGFβ1, transforming growth factor–β1; TNFα, tumor necrosis factor–α; DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche

    doi: 10.1126/sciadv.aba5536

    Figure Lengend Snippet: ( A ) Schematic demonstrating the design of leukemia-on-a-chip that consists of three functional regions. ( B ) Whole scan of the resultant leukemic BM niche system, where the enlarged area shows the colocalization of murine B-ALL cells within the perivascular niche. The bottom insert shows colocalization in the endosteal niche. ( C ) Hematoxylin and eosin (H&E) staining image of the in vivo murine leukemic BM niche, with the enlarged area showing the colocalization of B-ALL cells within the perivascular and endosteal niches. ( D ) The chemoresistance was compared between the engineered human BM niches of ETV6-RUNX1 + REH and Ph + SUP-B15 B-ALL. Each drug concentration had three or more experimental replicates. ( E ) The cytokine profiles from two B-ALL blasts with and without niche cells were quantified using membrane-based enzyme-linked immunosorbent assay (ELISA) analysis. MCP-1, monocyte chemoattractant protein-1; MIG, monokine induced by gamma interferon. ( F ) Quantification of nuclear (Nuc)/cytoplasmic (Cyto) ratio of NF-κB in REH and SUP B-ALL within their respective niche models. The ratios for REH and SUP were manually measured from three experimental replicates ( n > 150). ( G ) Percentage of Ki67 + B-ALL cells, corresponding to (F). Data were collected from three experimental replicates. Unpaired t test (** P < 0.01, Mann-Whitney test). GCSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; GRO, growth-regulated oncogene; IL, interleukin; IFN-γ, interferon-γ; TGFβ1, transforming growth factor–β1; TNFα, tumor necrosis factor–α; DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Cytokine secretion profiles of niche cells were examined by using Mouse Cytokine Antibody Array membrane-based ELISA kit (AAM-CYT-3, RayBiotech) or Human Cytokine Antibody Array membrane-based ELISA kit (AAH-CYT-1, RayBiotech), according to the manufacturer’s protocols.

    Techniques: Functional Assay, Staining, In Vivo, Concentration Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    ( A ) Regional CXCR4 distribution on murine B-ALL cells cultured with or without niche cells. ( B ) Quantified result corresponding to (A). ( C ) Membrane-based ELISA analysis of CXCL12 expression level of niche cells (ECs and MSCs). ( D ) Quantified result corresponding to (C). ( E ) Representative image showing B-ALL cells colocalized with niche cells via VCAM-1/VLA-4 signaling. ( F ) Representative images showing nuclear translocation of NF-κB in B-ALL cells. ( G ) NF-κB activation in B-ALL cells with or without niche cells and under treatments with CXCR4 inhibitor, AMD3100 (AMD), and VLA-4 inhibitor, BIO. The ratios were manually quantified from three experimental replicates ( n > 200). One-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. ( H ) B-ALL cell viability cultured with or without niche cells. ( I ) Quantification of NF-κB activation in human NALM-6, 697, RS(4;11), and UOCB1 blasts within their leukemia niches. The ratios were manually measured from three experimental replicates ( N > 150). ( J ) Quantification of NF-κB activation in patient-derived B-ALL blasts within engineered leukemia niches. The ratios for various B-ALL blasts were manually measured from three technical replicates ( N > 20). Unpaired t test (* P < 0.05 and ** P < 0.01, Mann-Whitney test).

    Journal: Science Advances

    Article Title: Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche

    doi: 10.1126/sciadv.aba5536

    Figure Lengend Snippet: ( A ) Regional CXCR4 distribution on murine B-ALL cells cultured with or without niche cells. ( B ) Quantified result corresponding to (A). ( C ) Membrane-based ELISA analysis of CXCL12 expression level of niche cells (ECs and MSCs). ( D ) Quantified result corresponding to (C). ( E ) Representative image showing B-ALL cells colocalized with niche cells via VCAM-1/VLA-4 signaling. ( F ) Representative images showing nuclear translocation of NF-κB in B-ALL cells. ( G ) NF-κB activation in B-ALL cells with or without niche cells and under treatments with CXCR4 inhibitor, AMD3100 (AMD), and VLA-4 inhibitor, BIO. The ratios were manually quantified from three experimental replicates ( n > 200). One-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. ( H ) B-ALL cell viability cultured with or without niche cells. ( I ) Quantification of NF-κB activation in human NALM-6, 697, RS(4;11), and UOCB1 blasts within their leukemia niches. The ratios were manually measured from three experimental replicates ( N > 150). ( J ) Quantification of NF-κB activation in patient-derived B-ALL blasts within engineered leukemia niches. The ratios for various B-ALL blasts were manually measured from three technical replicates ( N > 20). Unpaired t test (* P < 0.05 and ** P < 0.01, Mann-Whitney test).

    Article Snippet: Cytokine secretion profiles of niche cells were examined by using Mouse Cytokine Antibody Array membrane-based ELISA kit (AAM-CYT-3, RayBiotech) or Human Cytokine Antibody Array membrane-based ELISA kit (AAH-CYT-1, RayBiotech), according to the manufacturer’s protocols.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Translocation Assay, Activation Assay, Derivative Assay, MANN-WHITNEY

    ( A ) Membrane-based ELISA analysis of CXCL12 secretion of ECs (top left) and MSCs (top right). Western blotting of CXCL12 expression of ECs (bottom left) and MSCs (bottom right). ( B ) VCAM-1 expression of ECs and the quantification result ( n > 200). ( C ) OPN expression of 2D cultured MSCs and the quantification result ( N > 200). ( D ) The correlation between distance of B-ALL to MSCs and dye-retaining ability of B-ALL ( n > 120). B-ALL with lower DiD intensity was excluded as indicated by the dashed line. ( E and F ) The flow cytometric images showing DiD dye retained in B-ALL, i.e., low (Lo), middle (Mid), and high (Hi) intensity. FSC-A, forward scatter area. ( G ) The representative images showing p21 expression in B-ALL located in the perivascular and endosteal niches ( N = 54). ( H ) B-ALL viability in the two niches treated with different drugs. ( I ) NF-κB activation in REH after coculture with hematopoietic cells. ( J ) REH viability cultured with hematopoietic cells after 48-hour treatment of 20 nM VCR. ( K ) Representative images of CD34 + HSPC within (REH) or without (Control) the leukemia niche at days 1 and 9. ( L ) Quantified number of CD34 + cells. Unpaired t test (* P < 0.05 and ** P < 0.01, Mann-Whitney test). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; a.u., arbitrary units.

    Journal: Science Advances

    Article Title: Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche

    doi: 10.1126/sciadv.aba5536

    Figure Lengend Snippet: ( A ) Membrane-based ELISA analysis of CXCL12 secretion of ECs (top left) and MSCs (top right). Western blotting of CXCL12 expression of ECs (bottom left) and MSCs (bottom right). ( B ) VCAM-1 expression of ECs and the quantification result ( n > 200). ( C ) OPN expression of 2D cultured MSCs and the quantification result ( N > 200). ( D ) The correlation between distance of B-ALL to MSCs and dye-retaining ability of B-ALL ( n > 120). B-ALL with lower DiD intensity was excluded as indicated by the dashed line. ( E and F ) The flow cytometric images showing DiD dye retained in B-ALL, i.e., low (Lo), middle (Mid), and high (Hi) intensity. FSC-A, forward scatter area. ( G ) The representative images showing p21 expression in B-ALL located in the perivascular and endosteal niches ( N = 54). ( H ) B-ALL viability in the two niches treated with different drugs. ( I ) NF-κB activation in REH after coculture with hematopoietic cells. ( J ) REH viability cultured with hematopoietic cells after 48-hour treatment of 20 nM VCR. ( K ) Representative images of CD34 + HSPC within (REH) or without (Control) the leukemia niche at days 1 and 9. ( L ) Quantified number of CD34 + cells. Unpaired t test (* P < 0.05 and ** P < 0.01, Mann-Whitney test). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; a.u., arbitrary units.

    Article Snippet: Cytokine secretion profiles of niche cells were examined by using Mouse Cytokine Antibody Array membrane-based ELISA kit (AAM-CYT-3, RayBiotech) or Human Cytokine Antibody Array membrane-based ELISA kit (AAH-CYT-1, RayBiotech), according to the manufacturer’s protocols.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Cell Culture, Activation Assay, MANN-WHITNEY