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vectashield fluorescent mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield fluorescent mounting medium with dapi
    Vectashield Fluorescent Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/medium+for+fluorescence/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/10__1002_slash_pgr2__70054-63-4-10
    Average 98 stars, based on 22085 article reviews
    vectashield fluorescent mounting medium with dapi - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separation
    Article Snippet: Coverslips were washed 3 × 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa Fluor secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 × 10 min with 0.05% PBST followed by 2 × 1 min with PBS before mounting. .. Nuclear DNA was either stained with 0.5 μg/ml DAPI for 10 min at room temperature and then mounted with mounting medium for fluorescence (Vector, H-1000) or directly mounted in mounting medium with DAPI for fluorescence (Vector, H-1200) before imaging. .. Coverslips were washed 3 × 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa Fluor secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 × 10 min with 0.05% PBST followed by 2 × 1 min with PBS before mounting.

    Article Title: Alternative splicing modulates chromatin interactome and phase separation of the RIF1 C-terminal domain
    Article Snippet: Coverslips were washed 3 X 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa FluorTM secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 X 10 min with 0.05% PBST followed by 2 X 1 min with PBS before mounting. .. Nuclear DNA was either stained with 0.5 μg/ml DAPI for 10 min at room temperature and then mounted with mounting medium for fluorescence (Vector, H-1000), or directly mounted in mounting medium with DAPI for fluorescence (Vector, H-1200) before imaging. .. Images were acquired using Nikon A1RS or Nikon AX confocal microscopes under the desired objectives.

    Fluorescence:

    Article Title: DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separation
    Article Snippet: Coverslips were washed 3 × 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa Fluor secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 × 10 min with 0.05% PBST followed by 2 × 1 min with PBS before mounting. .. Nuclear DNA was either stained with 0.5 μg/ml DAPI for 10 min at room temperature and then mounted with mounting medium for fluorescence (Vector, H-1000) or directly mounted in mounting medium with DAPI for fluorescence (Vector, H-1200) before imaging. .. Coverslips were washed 3 × 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa Fluor secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 × 10 min with 0.05% PBST followed by 2 × 1 min with PBS before mounting.

    Article Title: A single-cell landscape of pre- and post-menopausal high-grade serous ovarian cancer ascites
    Article Snippet: Finally, slides were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (F6057, Sigma Aldrich). .. Drop mounting medium for fluorescence (VECTASHIELD H-1000, Vector lab) on the slide and mounted coverslips. .. We used ZEISS 980 for image acquisition, ZEN 2.3 (blue edition) image analysis software was used to separate positive from negative cells.

    Article Title: Expression of the VapD protein by Helicobacter pylori during intracellular infection
    Article Snippet: The cells were washed with 1× PBS and incubated with anti-mouse Alexa Fluor 594 (Life Technologies, USA) and anti-rabbit Alexa Fluor 488 (Life Technologies) antibodies, both of which were developed in goats and diluted 1:100 in 1× PBS, for 2 h at room temperature. .. Finally, the cells were washed with 1× PBS, incubated with DAPI for 1 min, washed and mounted with mounting medium for fluorescence (Vectashield Antifade Mounting Medium, Vector Laboratories). .. The samples were observed under a Nikon Eclipse E600 epifluorescence microscope (Nikon, USA) or Zeiss LSM 800 Confocal Laser Scanning Microscope (Zeiss, Germany).

    Article Title: Alternative splicing modulates chromatin interactome and phase separation of the RIF1 C-terminal domain
    Article Snippet: Coverslips were washed 3 X 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa FluorTM secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 X 10 min with 0.05% PBST followed by 2 X 1 min with PBS before mounting. .. Nuclear DNA was either stained with 0.5 μg/ml DAPI for 10 min at room temperature and then mounted with mounting medium for fluorescence (Vector, H-1000), or directly mounted in mounting medium with DAPI for fluorescence (Vector, H-1200) before imaging. .. Images were acquired using Nikon A1RS or Nikon AX confocal microscopes under the desired objectives.

    Article Title: Repurposing Niclosamide as a Novel Anti-SARS-CoV-2 Drug by Restricting Entry Protein CD147
    Article Snippet: .. Slides were mounted with SlowFade Gold antifade reagent (Invitrogen, Carlsbad, CA, USA, Cat# S36938) and DAPI containing mounting medium for fluorescence (Vector Laboratories, Newark, CA, USA, Cat# H-1200). .. Cells were imaged using an Olympus IX71 microscope using DP Controller and DP Manager software (3.3.1.222).

    Article Title: Short-term fibronectin treatment induces endothelial-like and angiogenic properties in monocyte-derived immature dendritic cells: involvement of intracellular VEGF and MAPK regulation.
    Article Snippet: Fibronectin (FN) is an extracellular matrix protein promoting cell proliferation, adhesion, and survival and is localized in the intimal layer of normal and atherosclerotic blood vessels.. Dendritic cells (DCs) are potent antigen-presenting cells located in healthy and diseased intima, and thus may predispose arteries to atherosclerosis.. Besides their pro-atherogenic activities DCs can promote neovascularization, by releasing pro-angiogenic mediators and/or by trans-differentiating into endothelial-like cells.

    Article Title: Expression of the VapD protein by Helicobacter pylori during intracellular infection.
    Article Snippet: The cells were washed with 1× PBS and incubated with anti- mouse Alexa Fluor 594 (Life Technologies, USA) and anti- rabbit Alexa Fluor 488 (Life Technologies) antibodies, both of which were developed in goats and diluted 1:100 in 1× PBS, for 2 h at room temperature. .. Finally, the cells were washed with 1× PBS, incubated with DAPI for 1 min, washed and mounted with mounting medium for fluorescence (Vectashield Antifade Mounting Medium, Vector Laboratories). .. The samples were observed under a Nikon Eclipse E600 epifluorescence microscope (Nikon, USA) or Zeiss LSM 800 Confocal Laser Scanning Microscope (Zeiss, Germany).

    Article Title: Incidental Lewy body disease and preclinical Parkinson disease.
    Article Snippet: .. The sections were then coverslipped in mounting medium for fluorescence (Vector Shield H-1000; Vector Laboratories, Burlingame, California). ..

    Imaging:

    Article Title: DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separation
    Article Snippet: Coverslips were washed 3 × 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa Fluor secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 × 10 min with 0.05% PBST followed by 2 × 1 min with PBS before mounting. .. Nuclear DNA was either stained with 0.5 μg/ml DAPI for 10 min at room temperature and then mounted with mounting medium for fluorescence (Vector, H-1000) or directly mounted in mounting medium with DAPI for fluorescence (Vector, H-1200) before imaging. .. Coverslips were washed 3 × 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa Fluor secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 × 10 min with 0.05% PBST followed by 2 × 1 min with PBS before mounting.

    Article Title: Alternative splicing modulates chromatin interactome and phase separation of the RIF1 C-terminal domain
    Article Snippet: Coverslips were washed 3 X 10 min with 0.05% v/v PBST (PBS with 0.05% v/v Tween-20) before incubating with Alexa FluorTM secondary antibodies (Thermo Fisher #A11032, #A32733) at a dilution factor of 1:10,000 at room temperature for 45 min. Coverslips were washed 3 X 10 min with 0.05% PBST followed by 2 X 1 min with PBS before mounting. .. Nuclear DNA was either stained with 0.5 μg/ml DAPI for 10 min at room temperature and then mounted with mounting medium for fluorescence (Vector, H-1000), or directly mounted in mounting medium with DAPI for fluorescence (Vector, H-1200) before imaging. .. Images were acquired using Nikon A1RS or Nikon AX confocal microscopes under the desired objectives.

    Incubation:

    Article Title: Expression of the VapD protein by Helicobacter pylori during intracellular infection
    Article Snippet: The cells were washed with 1× PBS and incubated with anti-mouse Alexa Fluor 594 (Life Technologies, USA) and anti-rabbit Alexa Fluor 488 (Life Technologies) antibodies, both of which were developed in goats and diluted 1:100 in 1× PBS, for 2 h at room temperature. .. Finally, the cells were washed with 1× PBS, incubated with DAPI for 1 min, washed and mounted with mounting medium for fluorescence (Vectashield Antifade Mounting Medium, Vector Laboratories). .. The samples were observed under a Nikon Eclipse E600 epifluorescence microscope (Nikon, USA) or Zeiss LSM 800 Confocal Laser Scanning Microscope (Zeiss, Germany).

    Article Title: Expression of the VapD protein by Helicobacter pylori during intracellular infection.
    Article Snippet: The cells were washed with 1× PBS and incubated with anti- mouse Alexa Fluor 594 (Life Technologies, USA) and anti- rabbit Alexa Fluor 488 (Life Technologies) antibodies, both of which were developed in goats and diluted 1:100 in 1× PBS, for 2 h at room temperature. .. Finally, the cells were washed with 1× PBS, incubated with DAPI for 1 min, washed and mounted with mounting medium for fluorescence (Vectashield Antifade Mounting Medium, Vector Laboratories). .. The samples were observed under a Nikon Eclipse E600 epifluorescence microscope (Nikon, USA) or Zeiss LSM 800 Confocal Laser Scanning Microscope (Zeiss, Germany).

    Microscopy:

    Article Title: Short-term fibronectin treatment induces endothelial-like and angiogenic properties in monocyte-derived immature dendritic cells: involvement of intracellular VEGF and MAPK regulation.
    Article Snippet: Fibronectin (FN) is an extracellular matrix protein promoting cell proliferation, adhesion, and survival and is localized in the intimal layer of normal and atherosclerotic blood vessels.. Dendritic cells (DCs) are potent antigen-presenting cells located in healthy and diseased intima, and thus may predispose arteries to atherosclerosis.. Besides their pro-atherogenic activities DCs can promote neovascularization, by releasing pro-angiogenic mediators and/or by trans-differentiating into endothelial-like cells.



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