vectashield vibrance antifade mounting media with dapi (Vector Laboratories)

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Vector Laboratories vectashield vibrance antifade mounting media with dapi

Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Vectashield Vibrance Antifade Mounting Media With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/media+with+dapi/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit+with+DAPI/pmc12810342-56-10-18
Average 96 stars, based on 160 article reviews

vectashield vibrance antifade mounting media with dapi - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis"

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

Journal: Journal of Translational Autoimmunity

doi: 10.1016/j.jtauto.2025.100345

Figure Legend Snippet: Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Techniques Used: Fluorescence

Imaging:

Article Title: Rolipram‐Loaded PgP Nanotherapeutics via Intrathecal Administration Reduces Secondary Injury in a Rat Acute Moderate Contusion SCI Model
Article Snippet: Goat anti‐mouse Cy3‐conjugated secondary antibodies (1:200; Cat.# 115–165‐003, Jackson ImmunoResearch, West Grove, PA) were used for CREB, NeuN and Arg1 and AlexaFluor 488‐conjugated goat anti‐rabbit secondary antibodies (1:500; Cat.# A‐11008, Thermo Fisher Scientific, Hampton, NH) were used for pCREB, GFAP and CD68. .. For mounting, imaging, and analysis, anti‐fade mounting media with DAPI (Vectashield, Vector, Newark, CA) was applied and coverslips placed and sealed with clear nail polish (OPI, Calabasas, CA). .. Images of pCREB, CREB, NeuN, CD68, Arg1 and GFAP stained sections were captured using a Keyence BZ‐X810 microscope (Original magnification: 100X) and stitched by BZ‐X800 Analyzer.

Article Title: Near-infrared photoimmunotherapy for effective elimination of ovarian cancer cells by inducing immunogenic cell death
Article Snippet: After that the sections were incubated with Alexa Fluor 488 Tyramide (Invitrogen, B40953 ), Alexa Fluor 546 Tyramine (Invitrogen, B40954 ), Alexa Fluor 647 Tyramide (Invitrogen, B40958 ), iFluor 750 Styramide (AAT Bioquest, #45065) according to the abovementioned procedure. .. Finally, the slides were prepared for fluorescence imaging by mounting them with mounting media with DAPI (#H-1200, Vector Laboratories). .. For binding analysis of scFv-SNAP fusion proteins, scFv-Erbitux-SNAP, scFv-Hereceptin-SNAP, scFv-Farletuzumab-SNAP, scFv-Tisotuzumab-SNAP, and scFv-Sacituzumab-SNAP were conjugated with SNAP-biotin (New England Biolabs, #S9110S) by incubating at a 1:3 molar ratio at room temperature for 2 h. Tissue staining was performed according to the above-mentioned procedure except instead of using secondary antibody, streptavidin-conjugated HRP was used.

Staining:

Article Title: BMI-1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma.
Article Snippet: Primary antibodies were used against BMI-1 (1 : 500, Cat# 6964, RRID:AB_10828713; Cell signaling), RING1B (1 : 500, Cat# 5694, RRID:AB_10705604; Cell signaling), H3 S10-P (1 : 500, Cat# 9706, RRID:AB_331748; Cell signaling), and Cyclin B1 (1 : 500, Cat# 4138, RRID: AB_2072132; Cell signaling) were stained with corresponding secondary antibodies, Alexa Fluor 488 AffiniPureTM Donkey Anti-Rabbit IgG (H + L) (1 : 500, Cat# 711-545- 152, RRID:AB_2313584; Jackson ImmunoResearch, West Grove, PA, USA) or Alexa Fluor 594 AffiniPureTM Donkey Anti-Mouse IgG (H + L) (1 : 500, Cat# 715-585-150, RRID:AB_2340854, Jackson ImmunoResearch). .. For nuclear staining, cells were embedded with mounting media with DAPI (H1200; Vector Laboratories, Newark, CA, USA). .. Images were captured with a 609 oil objective on a Nikon Eclipse Ti confocal microscope.

Article Title: Treatment of Fuchs' endothelial corneal dystrophy
Article Snippet: .. On the next day, cells were washed twice with wash buffer at 37° C. for 15 min, and then stained with mounting media with DAPI (H-1500; Vector Labs). .. Cells were imaged at 60× magnification using a Widefield Deltavision microscope.

Article Title: Rolipram‐Loaded PgP Nanotherapeutics via Intrathecal Administration Reduces Secondary Injury in a Rat Acute Moderate Contusion SCI Model
Article Snippet: .. Sections (Total 7 sections/rat, 1.2, 2.4, and 3.6 mm distances from the epicenter on both rostral (+) and caudal (−) sides) were selected and were stained by the terminal deoxynucleotidyl transferase‐mediated dUTP nicked end labeling (TUNEL) method using an ApopTag Plus Fluorescein in situ Apoptosis Detection Kit (S7111, EMD Millipore, Temecula, CA, USA) and anti‐fade mounting media with DAPI (Vectashield, Vector, Newark, CA) was applied and coverslips placed and sealed with clear nail polish (OPI, Calabasas, CA). .. Stained sections were imaged at 100X using a Keyence fluorescence microscope and TUNEL positive cells counted using NIH image software as described above.

Fluorescence:

Article Title: The Lysine Demethylase KDM4C Is an Oncogenic Driver and Regulates ERK Activity in KRAS-Mutant Pancreatic Ductal Adenocarcinoma
Article Snippet: Secondary antibodies were obtained from Abcam and used at 1:300 dilution; anti-rat IgG (Cy3, cat. #ab98416, RRID: AB_10673341), anti-mouse IgG (FITC, cat. #ab6785, RRID: AB_955241), anti-mouse IgG (Cy3, cat. #ab97035, RRID: AB_10680176), and anti-rabbit IgG (FITC, cat. #ab6717, RRID: AB_955238). .. Samples were mounted with fluorescence mounting media with DAPI (Vector Laboratories, cat. #H-1200-10) and left to dry overnight. .. Slides were imaged on the Olympus confocal microscope and quantified with ImageJ.

End Labeling:

Article Title: Rolipram‐Loaded PgP Nanotherapeutics via Intrathecal Administration Reduces Secondary Injury in a Rat Acute Moderate Contusion SCI Model
Article Snippet: .. Sections (Total 7 sections/rat, 1.2, 2.4, and 3.6 mm distances from the epicenter on both rostral (+) and caudal (−) sides) were selected and were stained by the terminal deoxynucleotidyl transferase‐mediated dUTP nicked end labeling (TUNEL) method using an ApopTag Plus Fluorescein in situ Apoptosis Detection Kit (S7111, EMD Millipore, Temecula, CA, USA) and anti‐fade mounting media with DAPI (Vectashield, Vector, Newark, CA) was applied and coverslips placed and sealed with clear nail polish (OPI, Calabasas, CA). .. Stained sections were imaged at 100X using a Keyence fluorescence microscope and TUNEL positive cells counted using NIH image software as described above.

TUNEL Assay:

Article Title: Rolipram‐Loaded PgP Nanotherapeutics via Intrathecal Administration Reduces Secondary Injury in a Rat Acute Moderate Contusion SCI Model
Article Snippet: .. Sections (Total 7 sections/rat, 1.2, 2.4, and 3.6 mm distances from the epicenter on both rostral (+) and caudal (−) sides) were selected and were stained by the terminal deoxynucleotidyl transferase‐mediated dUTP nicked end labeling (TUNEL) method using an ApopTag Plus Fluorescein in situ Apoptosis Detection Kit (S7111, EMD Millipore, Temecula, CA, USA) and anti‐fade mounting media with DAPI (Vectashield, Vector, Newark, CA) was applied and coverslips placed and sealed with clear nail polish (OPI, Calabasas, CA). .. Stained sections were imaged at 100X using a Keyence fluorescence microscope and TUNEL positive cells counted using NIH image software as described above.

In Situ:

Article Title: Rolipram‐Loaded PgP Nanotherapeutics via Intrathecal Administration Reduces Secondary Injury in a Rat Acute Moderate Contusion SCI Model
Article Snippet: .. Sections (Total 7 sections/rat, 1.2, 2.4, and 3.6 mm distances from the epicenter on both rostral (+) and caudal (−) sides) were selected and were stained by the terminal deoxynucleotidyl transferase‐mediated dUTP nicked end labeling (TUNEL) method using an ApopTag Plus Fluorescein in situ Apoptosis Detection Kit (S7111, EMD Millipore, Temecula, CA, USA) and anti‐fade mounting media with DAPI (Vectashield, Vector, Newark, CA) was applied and coverslips placed and sealed with clear nail polish (OPI, Calabasas, CA). .. Stained sections were imaged at 100X using a Keyence fluorescence microscope and TUNEL positive cells counted using NIH image software as described above.

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