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mcsf growth factor  (PeproTech)


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    Structured Review

    PeproTech mcsf growth factor
    Mcsf Growth Factor, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcsf+growth+factor/mcsf+growth+factor/10__3390_slash_jfb16060220-78-9-1
    Average 90 stars, based on 1 article reviews
    mcsf growth factor - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collagenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 d. After day 7, supernatant was harvested daily, and samples with p27 1 ng/ml were filtered (MW cutoff 30 kDa) and filtrates stored at 80°C.

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collegenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 days.

    Article Title: Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis
    Article Snippet: Cells were cultured in medium containing α‐MEM supplemented with 10% heat‐inactivated FBS, 5% penicillin/streptomycin solution, 5% L‐glutamine, 25 ng/mL MCSF (Peprotech) for 6 days.

    Article Title: Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation.
    Article Snippet: RPMI 1640 medium supplemented with MCSF (20 ng/mL, PEPROTECH, USA) and 10 % FBS was used to resuspend the cells before culture at a concentration of 2 × H. Jiang et al. Materials Today Bio 27 (2024) 101151 106 cells/mL.

    Article Title: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.
    Article Snippet: The mixture was centrifuged again for 4 min at 300 × g. Cells were then plated on a 24- well plate with Iscove’s Modified Dulbecco’s Medium (IMDM), 10% PBS+ P/S, and 10 ngmL−1 MCSF (Peprotech).

    Article Title: Notch signal deficiency alleviates hypertrophic scar formation after wound healing through the inhibition of inflammation.
    Article Snippet: Pathological scar is a common complication after wound healing.. One of the most important factors that affects scar formation is inflammation.. During this process, macrophages play a critical role in the wound healing process, as well as in scar formation.

    Article Title: A Modular Biomimetic Preclinical Platform to Elucidate the Interaction Between Cancer Cells and the Bone Metastatic Niche
    Article Snippet: 310-01; Peprotech, Rocky Hill, NJ, USA) was added with MCSF.

    Expressing:

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collagenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 d. After day 7, supernatant was harvested daily, and samples with p27 1 ng/ml were filtered (MW cutoff 30 kDa) and filtrates stored at 80°C.

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collegenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 days.

    Article Title: Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis
    Article Snippet: Cells were cultured in medium containing α‐MEM supplemented with 10% heat‐inactivated FBS, 5% penicillin/streptomycin solution, 5% L‐glutamine, 25 ng/mL MCSF (Peprotech) for 6 days.

    Article Title: Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation.
    Article Snippet: RPMI 1640 medium supplemented with MCSF (20 ng/mL, PEPROTECH, USA) and 10 % FBS was used to resuspend the cells before culture at a concentration of 2 × H. Jiang et al. Materials Today Bio 27 (2024) 101151 106 cells/mL.

    Article Title: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.
    Article Snippet: The mixture was centrifuged again for 4 min at 300 × g. Cells were then plated on a 24- well plate with Iscove’s Modified Dulbecco’s Medium (IMDM), 10% PBS+ P/S, and 10 ngmL−1 MCSF (Peprotech).

    Article Title: Notch signal deficiency alleviates hypertrophic scar formation after wound healing through the inhibition of inflammation.
    Article Snippet: Pathological scar is a common complication after wound healing.. One of the most important factors that affects scar formation is inflammation.. During this process, macrophages play a critical role in the wound healing process, as well as in scar formation.

    Article Title: A Modular Biomimetic Preclinical Platform to Elucidate the Interaction Between Cancer Cells and the Bone Metastatic Niche
    Article Snippet: 310-01; Peprotech, Rocky Hill, NJ, USA) was added with MCSF.

    Control:

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collagenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 d. After day 7, supernatant was harvested daily, and samples with p27 1 ng/ml were filtered (MW cutoff 30 kDa) and filtrates stored at 80°C.

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collegenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 days.

    Article Title: Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis
    Article Snippet: Cells were cultured in medium containing α‐MEM supplemented with 10% heat‐inactivated FBS, 5% penicillin/streptomycin solution, 5% L‐glutamine, 25 ng/mL MCSF (Peprotech) for 6 days.

    Article Title: Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation.
    Article Snippet: RPMI 1640 medium supplemented with MCSF (20 ng/mL, PEPROTECH, USA) and 10 % FBS was used to resuspend the cells before culture at a concentration of 2 × H. Jiang et al. Materials Today Bio 27 (2024) 101151 106 cells/mL.

    Article Title: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.
    Article Snippet: The mixture was centrifuged again for 4 min at 300 × g. Cells were then plated on a 24- well plate with Iscove’s Modified Dulbecco’s Medium (IMDM), 10% PBS+ P/S, and 10 ngmL−1 MCSF (Peprotech).

    Article Title: Notch signal deficiency alleviates hypertrophic scar formation after wound healing through the inhibition of inflammation.
    Article Snippet: Pathological scar is a common complication after wound healing.. One of the most important factors that affects scar formation is inflammation.. During this process, macrophages play a critical role in the wound healing process, as well as in scar formation.

    Article Title: A Modular Biomimetic Preclinical Platform to Elucidate the Interaction Between Cancer Cells and the Bone Metastatic Niche
    Article Snippet: 310-01; Peprotech, Rocky Hill, NJ, USA) was added with MCSF.

    Recombinant:

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collagenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 d. After day 7, supernatant was harvested daily, and samples with p27 1 ng/ml were filtered (MW cutoff 30 kDa) and filtrates stored at 80°C.

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collegenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 days.

    Article Title: Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis
    Article Snippet: Cells were cultured in medium containing α‐MEM supplemented with 10% heat‐inactivated FBS, 5% penicillin/streptomycin solution, 5% L‐glutamine, 25 ng/mL MCSF (Peprotech) for 6 days.

    Article Title: Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation.
    Article Snippet: RPMI 1640 medium supplemented with MCSF (20 ng/mL, PEPROTECH, USA) and 10 % FBS was used to resuspend the cells before culture at a concentration of 2 × H. Jiang et al. Materials Today Bio 27 (2024) 101151 106 cells/mL.

    Article Title: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.
    Article Snippet: The mixture was centrifuged again for 4 min at 300 × g. Cells were then plated on a 24- well plate with Iscove’s Modified Dulbecco’s Medium (IMDM), 10% PBS+ P/S, and 10 ngmL−1 MCSF (Peprotech).

    Article Title: Notch signal deficiency alleviates hypertrophic scar formation after wound healing through the inhibition of inflammation.
    Article Snippet: Pathological scar is a common complication after wound healing.. One of the most important factors that affects scar formation is inflammation.. During this process, macrophages play a critical role in the wound healing process, as well as in scar formation.

    Article Title: A Modular Biomimetic Preclinical Platform to Elucidate the Interaction Between Cancer Cells and the Bone Metastatic Niche
    Article Snippet: 310-01; Peprotech, Rocky Hill, NJ, USA) was added with MCSF.

    Negative Control:

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collagenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 d. After day 7, supernatant was harvested daily, and samples with p27 1 ng/ml were filtered (MW cutoff 30 kDa) and filtrates stored at 80°C.

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collegenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 days.

    Article Title: Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis
    Article Snippet: Cells were cultured in medium containing α‐MEM supplemented with 10% heat‐inactivated FBS, 5% penicillin/streptomycin solution, 5% L‐glutamine, 25 ng/mL MCSF (Peprotech) for 6 days.

    Article Title: Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation.
    Article Snippet: RPMI 1640 medium supplemented with MCSF (20 ng/mL, PEPROTECH, USA) and 10 % FBS was used to resuspend the cells before culture at a concentration of 2 × H. Jiang et al. Materials Today Bio 27 (2024) 101151 106 cells/mL.

    Article Title: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.
    Article Snippet: The mixture was centrifuged again for 4 min at 300 × g. Cells were then plated on a 24- well plate with Iscove’s Modified Dulbecco’s Medium (IMDM), 10% PBS+ P/S, and 10 ngmL−1 MCSF (Peprotech).

    Article Title: Notch signal deficiency alleviates hypertrophic scar formation after wound healing through the inhibition of inflammation.
    Article Snippet: Pathological scar is a common complication after wound healing.. One of the most important factors that affects scar formation is inflammation.. During this process, macrophages play a critical role in the wound healing process, as well as in scar formation.

    Article Title: A Modular Biomimetic Preclinical Platform to Elucidate the Interaction Between Cancer Cells and the Bone Metastatic Niche
    Article Snippet: 310-01; Peprotech, Rocky Hill, NJ, USA) was added with MCSF.

    RNA Extraction:

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collagenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 d. After day 7, supernatant was harvested daily, and samples with p27 1 ng/ml were filtered (MW cutoff 30 kDa) and filtrates stored at 80°C.

    Article Title: An Integrated Systems Analysis Implicates EGR1 Downregulation in Simian Immunodeficiency Virus Encephalitis-Induced Neural Dysfunction
    Article Snippet: Macrophages/microglia were isolated by Percoll gradient from disrupted, collegenase-digested brains of rhesus macaques with SIVE and maintained in RPMI 1640 supplemented with MCSF at 50 ng/ml (Peprotech) up to 30 days.

    Article Title: Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis
    Article Snippet: Cells were cultured in medium containing α‐MEM supplemented with 10% heat‐inactivated FBS, 5% penicillin/streptomycin solution, 5% L‐glutamine, 25 ng/mL MCSF (Peprotech) for 6 days.

    Article Title: Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation.
    Article Snippet: RPMI 1640 medium supplemented with MCSF (20 ng/mL, PEPROTECH, USA) and 10 % FBS was used to resuspend the cells before culture at a concentration of 2 × H. Jiang et al. Materials Today Bio 27 (2024) 101151 106 cells/mL.

    Article Title: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.
    Article Snippet: The mixture was centrifuged again for 4 min at 300 × g. Cells were then plated on a 24- well plate with Iscove’s Modified Dulbecco’s Medium (IMDM), 10% PBS+ P/S, and 10 ngmL−1 MCSF (Peprotech).

    Article Title: Notch signal deficiency alleviates hypertrophic scar formation after wound healing through the inhibition of inflammation.
    Article Snippet: Pathological scar is a common complication after wound healing.. One of the most important factors that affects scar formation is inflammation.. During this process, macrophages play a critical role in the wound healing process, as well as in scar formation.

    Article Title: A Modular Biomimetic Preclinical Platform to Elucidate the Interaction Between Cancer Cells and the Bone Metastatic Niche
    Article Snippet: 310-01; Peprotech, Rocky Hill, NJ, USA) was added with MCSF.



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    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL <t>MCSF</t> alone or 25 ng/mL MCSF and 100 <t>ng/mL</t> <t>RANKL</t> for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.
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    Image Search Results


    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Journal: PeerJ

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    doi: 10.7717/peerj.14814

    Figure Lengend Snippet: Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Techniques: Derivative Assay, Cell Culture, Lysis, RNA Extraction, Staining

    (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Journal: PeerJ

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    doi: 10.7717/peerj.14814

    Figure Lengend Snippet: (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Techniques: Transformation Assay