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sfgfp lamp1 mcherry addgene plasmid  (Addgene inc)


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    Addgene inc sfgfp lamp1 mcherry addgene plasmid
    Sfgfp Lamp1 Mcherry Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+tom20/Tom20-CIB-GFP+(Plasmid+%23117242)/pm41838720-407-48-49
    Average 90 stars, based on 3 article reviews
    sfgfp lamp1 mcherry addgene plasmid - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title:
    Article Snippet: Manufacturer Dilution/ Working concentration Mouse anti-ATP5A (monoclonal) ab14748 Abcam 1:100 Rabbit anti-RUNX2 (monoclonal) 12556S CST 1:1000 Mouse anti-Filamin A (monoclonal) F6682 Sigma 1:100 Rabbit anti-YAP (monoclonal) 14074S CST 1:200 DAPI (4′,6-diamidino-2-phenylindole) D1306 Invitrogen 1 μg ml-1 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor-647 Conjugate) 4414S CST Same as per primary antibody dilution Anti-rabbit IgG(H+L), F(ab') Fragment (Alexa Fluor 594 Conjugate) 8889 CST Alexa Fluor 647, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A21236 Invitrogen Alexa Fluor 568, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A11031 Invitrogen Alexa Fluor 488, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A-11001 Invitrogen Alexa Fluor 488, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody A-11008 Invitrogen Mito Flipper-TR CY-SC023 Cytoskeleton 1 μM SiR-Actin CY-SC006 Cytoskeleton 50 nM Alexa Fluor 647 Phalloidin 8940 CST 1:40 3 Mitotracker Green 9074S CST 100 nM Mitotracker Red CMXRos 9082S CST 100 nM .. Plasmid Source Identifier Comment mApple-FilaminA-N-9 Addgene 54901 Filamin overexpression mApple-dnFLNA This work DOI: 10.1073/pnas.110 421 1108 Human FLNA Repeat [19- 22]-HA cloned into mApple C1 mApple-C1 Addgene 54631 C1 cloning vector (Empty backbone) Strep-KifC1*-mCherry Addgene 120169 KIF-C1 based retrograde motor tagged with Streptavidin and mCherry mCherry-KIF5B*-strep Addgene 120164 KIF-5B based anterograde motor tagged with Streptavidin and mCherry TOM20*-SBP-GFP Addgene 120173 Mitochondrial OMM protein Tom20 tagged with SBP and GFP Mito-Dendra 2 Vaishnavi Ananthanarayanan 55796 Photo-convertible mitochondrial marker Vinculin T (pCAG-C1- Vcl-T) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Vinculin Tail domain overexpression Vinculin T12 (pCAG- C1-Vcl-T12) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Constitutively active Vinculin ..

    Over Expression:

    Article Title:
    Article Snippet: Manufacturer Dilution/ Working concentration Mouse anti-ATP5A (monoclonal) ab14748 Abcam 1:100 Rabbit anti-RUNX2 (monoclonal) 12556S CST 1:1000 Mouse anti-Filamin A (monoclonal) F6682 Sigma 1:100 Rabbit anti-YAP (monoclonal) 14074S CST 1:200 DAPI (4′,6-diamidino-2-phenylindole) D1306 Invitrogen 1 μg ml-1 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor-647 Conjugate) 4414S CST Same as per primary antibody dilution Anti-rabbit IgG(H+L), F(ab') Fragment (Alexa Fluor 594 Conjugate) 8889 CST Alexa Fluor 647, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A21236 Invitrogen Alexa Fluor 568, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A11031 Invitrogen Alexa Fluor 488, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A-11001 Invitrogen Alexa Fluor 488, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody A-11008 Invitrogen Mito Flipper-TR CY-SC023 Cytoskeleton 1 μM SiR-Actin CY-SC006 Cytoskeleton 50 nM Alexa Fluor 647 Phalloidin 8940 CST 1:40 3 Mitotracker Green 9074S CST 100 nM Mitotracker Red CMXRos 9082S CST 100 nM .. Plasmid Source Identifier Comment mApple-FilaminA-N-9 Addgene 54901 Filamin overexpression mApple-dnFLNA This work DOI: 10.1073/pnas.110 421 1108 Human FLNA Repeat [19- 22]-HA cloned into mApple C1 mApple-C1 Addgene 54631 C1 cloning vector (Empty backbone) Strep-KifC1*-mCherry Addgene 120169 KIF-C1 based retrograde motor tagged with Streptavidin and mCherry mCherry-KIF5B*-strep Addgene 120164 KIF-5B based anterograde motor tagged with Streptavidin and mCherry TOM20*-SBP-GFP Addgene 120173 Mitochondrial OMM protein Tom20 tagged with SBP and GFP Mito-Dendra 2 Vaishnavi Ananthanarayanan 55796 Photo-convertible mitochondrial marker Vinculin T (pCAG-C1- Vcl-T) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Vinculin Tail domain overexpression Vinculin T12 (pCAG- C1-Vcl-T12) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Constitutively active Vinculin ..

    Clone Assay:

    Article Title:
    Article Snippet: Manufacturer Dilution/ Working concentration Mouse anti-ATP5A (monoclonal) ab14748 Abcam 1:100 Rabbit anti-RUNX2 (monoclonal) 12556S CST 1:1000 Mouse anti-Filamin A (monoclonal) F6682 Sigma 1:100 Rabbit anti-YAP (monoclonal) 14074S CST 1:200 DAPI (4′,6-diamidino-2-phenylindole) D1306 Invitrogen 1 μg ml-1 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor-647 Conjugate) 4414S CST Same as per primary antibody dilution Anti-rabbit IgG(H+L), F(ab') Fragment (Alexa Fluor 594 Conjugate) 8889 CST Alexa Fluor 647, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A21236 Invitrogen Alexa Fluor 568, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A11031 Invitrogen Alexa Fluor 488, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A-11001 Invitrogen Alexa Fluor 488, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody A-11008 Invitrogen Mito Flipper-TR CY-SC023 Cytoskeleton 1 μM SiR-Actin CY-SC006 Cytoskeleton 50 nM Alexa Fluor 647 Phalloidin 8940 CST 1:40 3 Mitotracker Green 9074S CST 100 nM Mitotracker Red CMXRos 9082S CST 100 nM .. Plasmid Source Identifier Comment mApple-FilaminA-N-9 Addgene 54901 Filamin overexpression mApple-dnFLNA This work DOI: 10.1073/pnas.110 421 1108 Human FLNA Repeat [19- 22]-HA cloned into mApple C1 mApple-C1 Addgene 54631 C1 cloning vector (Empty backbone) Strep-KifC1*-mCherry Addgene 120169 KIF-C1 based retrograde motor tagged with Streptavidin and mCherry mCherry-KIF5B*-strep Addgene 120164 KIF-5B based anterograde motor tagged with Streptavidin and mCherry TOM20*-SBP-GFP Addgene 120173 Mitochondrial OMM protein Tom20 tagged with SBP and GFP Mito-Dendra 2 Vaishnavi Ananthanarayanan 55796 Photo-convertible mitochondrial marker Vinculin T (pCAG-C1- Vcl-T) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Vinculin Tail domain overexpression Vinculin T12 (pCAG- C1-Vcl-T12) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Constitutively active Vinculin ..

    Cloning:

    Article Title:
    Article Snippet: Manufacturer Dilution/ Working concentration Mouse anti-ATP5A (monoclonal) ab14748 Abcam 1:100 Rabbit anti-RUNX2 (monoclonal) 12556S CST 1:1000 Mouse anti-Filamin A (monoclonal) F6682 Sigma 1:100 Rabbit anti-YAP (monoclonal) 14074S CST 1:200 DAPI (4′,6-diamidino-2-phenylindole) D1306 Invitrogen 1 μg ml-1 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor-647 Conjugate) 4414S CST Same as per primary antibody dilution Anti-rabbit IgG(H+L), F(ab') Fragment (Alexa Fluor 594 Conjugate) 8889 CST Alexa Fluor 647, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A21236 Invitrogen Alexa Fluor 568, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A11031 Invitrogen Alexa Fluor 488, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A-11001 Invitrogen Alexa Fluor 488, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody A-11008 Invitrogen Mito Flipper-TR CY-SC023 Cytoskeleton 1 μM SiR-Actin CY-SC006 Cytoskeleton 50 nM Alexa Fluor 647 Phalloidin 8940 CST 1:40 3 Mitotracker Green 9074S CST 100 nM Mitotracker Red CMXRos 9082S CST 100 nM .. Plasmid Source Identifier Comment mApple-FilaminA-N-9 Addgene 54901 Filamin overexpression mApple-dnFLNA This work DOI: 10.1073/pnas.110 421 1108 Human FLNA Repeat [19- 22]-HA cloned into mApple C1 mApple-C1 Addgene 54631 C1 cloning vector (Empty backbone) Strep-KifC1*-mCherry Addgene 120169 KIF-C1 based retrograde motor tagged with Streptavidin and mCherry mCherry-KIF5B*-strep Addgene 120164 KIF-5B based anterograde motor tagged with Streptavidin and mCherry TOM20*-SBP-GFP Addgene 120173 Mitochondrial OMM protein Tom20 tagged with SBP and GFP Mito-Dendra 2 Vaishnavi Ananthanarayanan 55796 Photo-convertible mitochondrial marker Vinculin T (pCAG-C1- Vcl-T) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Vinculin Tail domain overexpression Vinculin T12 (pCAG- C1-Vcl-T12) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Constitutively active Vinculin ..

    Marker:

    Article Title:
    Article Snippet: Manufacturer Dilution/ Working concentration Mouse anti-ATP5A (monoclonal) ab14748 Abcam 1:100 Rabbit anti-RUNX2 (monoclonal) 12556S CST 1:1000 Mouse anti-Filamin A (monoclonal) F6682 Sigma 1:100 Rabbit anti-YAP (monoclonal) 14074S CST 1:200 DAPI (4′,6-diamidino-2-phenylindole) D1306 Invitrogen 1 μg ml-1 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor-647 Conjugate) 4414S CST Same as per primary antibody dilution Anti-rabbit IgG(H+L), F(ab') Fragment (Alexa Fluor 594 Conjugate) 8889 CST Alexa Fluor 647, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A21236 Invitrogen Alexa Fluor 568, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A11031 Invitrogen Alexa Fluor 488, Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody A-11001 Invitrogen Alexa Fluor 488, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody A-11008 Invitrogen Mito Flipper-TR CY-SC023 Cytoskeleton 1 μM SiR-Actin CY-SC006 Cytoskeleton 50 nM Alexa Fluor 647 Phalloidin 8940 CST 1:40 3 Mitotracker Green 9074S CST 100 nM Mitotracker Red CMXRos 9082S CST 100 nM .. Plasmid Source Identifier Comment mApple-FilaminA-N-9 Addgene 54901 Filamin overexpression mApple-dnFLNA This work DOI: 10.1073/pnas.110 421 1108 Human FLNA Repeat [19- 22]-HA cloned into mApple C1 mApple-C1 Addgene 54631 C1 cloning vector (Empty backbone) Strep-KifC1*-mCherry Addgene 120169 KIF-C1 based retrograde motor tagged with Streptavidin and mCherry mCherry-KIF5B*-strep Addgene 120164 KIF-5B based anterograde motor tagged with Streptavidin and mCherry TOM20*-SBP-GFP Addgene 120173 Mitochondrial OMM protein Tom20 tagged with SBP and GFP Mito-Dendra 2 Vaishnavi Ananthanarayanan 55796 Photo-convertible mitochondrial marker Vinculin T (pCAG-C1- Vcl-T) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Vinculin Tail domain overexpression Vinculin T12 (pCAG- C1-Vcl-T12) Narendrakumar Ramanan DOI: 10.1007/s00018- 021-03879-7 Constitutively active Vinculin ..



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    Image Search Results


    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

    Journal: bioRxiv

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    doi: 10.1101/2025.10.27.684728

    Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

    Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

    Techniques: Immunofluorescence, Staining

    (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

    Journal: bioRxiv

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    doi: 10.1101/2025.10.27.684728

    Figure Lengend Snippet: (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

    Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

    Techniques: Immunofluorescence, Staining

    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

    Journal: bioRxiv

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    doi: 10.1101/2025.10.27.684728

    Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

    Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

    Techniques: Immunofluorescence, Staining