mcherry coding sequences (TaKaRa)
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mcherry coding sequences
Mcherry Coding Sequences, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcherry+coding+sequence/pmc11244814-323-2-6
Average 86 stars, based on 1 article reviews
Mcherry Coding Sequences, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcherry+coding+sequence/pmc11244814-323-2-6
Average 86 stars, based on 1 article reviews
mcherry coding sequences - by Bioz Stars,
2026-09
86/100 stars
Images
1) Product Images from "Canonical and noncanonical roles of Hop1 are crucial for meiotic prophase in the fungus Sordaria macrospora"
Article Title: Canonical and noncanonical roles of Hop1 are crucial for meiotic prophase in the fungus Sordaria macrospora
Journal: PLOS Biology
doi: 10.1371/journal.pbio.3002705
Figure Legend Snippet: ( A ) Top: domain diagram of WT Hop1. Bottom: diagram of the deleted or mutated Hop1 sites in the 6 analyzed mutants (). ( B ) Protein localization and phenotypes of the hop1-HORMAΔ mutant. Top: In the hop1Δ background, the mutant protein tagged with mCherry (mC, left) is not visible along the axes, marked by Spo76-GFP (middle) and merge (right). Bottom: colocalization with Ecm11-GFP + Hei10-GFP (middle) indicates that only few SC segments (containing Hei10 foci, arrows) are formed in this mutant; right corresponding DAPI. ( C, D ) hop1-HORMAΔ SCs exhibit the same length ( C ) and Hei10 foci number ( D ) as hop1Δ . Mean and error bar (SD) are indicated for each set. Significance between WT, hop1Δ and hop1-HORMAΔ nuclei was established by Brown–Forsythe ANOVA test: ns = not significant, P -value > 0.05; n = 38 and 42 nuclei. ( E ) hop1-only-HORMA . Top: the HORMA domain alone is sufficient for axis localization of the protein but only as discontinuous segments (arrows) that colocalize with Spo76-GFP (arrows middle and merge right) in contrast to the continuous lines seen in WT with Hop1-mCherry (right). Bottom: colocalization of Hop1-only-HORMA-mC (left) with Ecm11-GFP and Hei10-GFP (middle) and merge (right) indicates that the visible mutant segments correspond to SCs. ( C ) The mutant SCs have the same length as hop1Δ SCs ( C ) but exhibit a slightly lower number of Hei10 foci ( D ): Brown–Forsythe ANOVA test, ns = not significant; n = 31 and 42 nuclei. Scale bars: 2 μm. The raw data underlying panels 6C and 6D are available in . SC, synaptonemal complex; WT, wild-type.
Techniques Used: Mutagenesis
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The mCherry-PMCA2b construct was created by replacing the enhanced green fluorescent protein (EGFP) fragment of pEGFP-C1PMCA2x/b (59) with the Article Title: Peritoneal Tumorigenesis and Inflammation are Ameliorated by Humidified-Warm Carbon Dioxide Insufflation in the Mouse Article Snippet: .. Murine stem cell virus (MSCV)-mCherry-CT26 cells were obtained by stable retroviral transduction with an MSCV-mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV-IRES-GFP (Addgene) with the Article Title: Targeted insertion in well-characterized Drosophila cell lines using φC31 integrase Article Snippet: .. Complete sequences for all of these plasmids are given in the supplemental materials; critical portions came from the following plasmids: insulators and eGFP from pStinger ( Barolo et al. 2000 ), P element ends from Carnegie4 , actin promoter and DHFR coding sequence from pUC-act-DHFR ( Segal et al. 1996 ), metallothionein promoter from pRmHa-1 ( Bunch et al. 1988 ), φC31 integrase coding sequence from pET11phiC31polyA ( Groth et al. 2004 ), HSV TK coding sequence from pAL119-TK ( Dewey et al. 1999 ) (from AddGene), attP sites from pXLBacII-attP-yellow forward (gift from Koen Venken), attB sites from attB-P[acman]-ApR ( Venken et al. 2006 ), Gateway entry cassette (from Invitrogen), Article Title: Apart from its known function, the plasma membrane Ca²⁺ATPase can regulate Ca²⁺ signaling by controlling phosphatidylinositol 4,5-bisphosphate levels. 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Sequences for all these plasmids are deposited in GenBank; critical portions came from the following plasmids: insulators and eGFP from pStinger ( Barolo et al. 2000 ), P-element ends from Carnegie4 (Rubin and Spradling 1983a), actin promoter and dihydrofolate reductase (DHFR) coding sequence from pUC-act-DHFR ( Segal et al. 1996 ), metallothionein promoter from pRmHa-1 ( Bunch et al. 1988 ), phiC31 integrase coding sequence from pET11phiC31polyA ( Groth et al. 2004 ), HSV TK coding sequence from pAL119-TK ( Dewey et al. 1999 ) (purchased from AddGene), attP sites from pXLBacII-attP-yellow forward (gift from Koen Venken), attB sites from attB-P[acman]-ApR ( Venken et al. 2006 ), Gateway entry cassette (purchased from Invitrogen), and Article Title: Experimental study of delivery of humidified‐warm carbon dioxide during open abdominal surgery Article Snippet: .. Murine stem cell virus (MSCV)‐mCherry‐CT26 cells were generated by stable retroviral transduction with an MSCV‐mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV‐IRES‐GFP (Addgene, Cambridge, Massachusetts, USA) with the Derivative Assay:Article Title: AMBRA1 is involved in T cell receptor-mediated metabolic reprogramming through an ATG7-independent pathway. Article Snippet: Metabolic reprogramming contributes to dynamic alteration of cell functions and characteristics.. In T cells, TCR-mediated signaling evokes metabolic reprogramming and autophagy.. AMBRA1 is known to serve in the facilitation of autophagy and quality control of mitochondria, but the role of AMBRA1 in T cell metabolic alteration is unknown. Article Title: Peritoneal Tumorigenesis and Inflammation are Ameliorated by Humidified-Warm Carbon Dioxide Insufflation in the Mouse Article Snippet: .. 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Murine stem cell virus (MSCV)‐mCherry‐CT26 cells were generated by stable retroviral transduction with an MSCV‐mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV‐IRES‐GFP (Addgene, Cambridge, Massachusetts, USA) with the Amplification:Article Title: Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection Article Snippet: pDM4 , Suicide vector; sacBR oriR6K Cm r , 40. .. pmCherry-1 , Vector used as template for amplification of the Construct:Article Title: Plasma membrane Ca²⁺-ATPases can shape the pattern of Ca²⁺ transients induced by store-operated Ca²⁺ entry. Article Snippet: .. The mCherry-PMCA2b construct was created by replacing the enhanced green fluorescent protein (EGFP) fragment of pEGFP-C1PMCA2x/b (59) with the Article Title: Apart from its known function, the plasma membrane Ca²⁺ATPase can regulate Ca²⁺ signaling by controlling phosphatidylinositol 4,5-bisphosphate levels. Article Snippet: Plasma membrane Ca ATPases (PMCAs, also known as ATP2B1–ATP2B4) are known targets of phosphatidylinositol 4,5bisphosphate [PtdIns(4,5)P2], but if and how they control the PtdIns(4,5)P2 pool has not been considered.. We demonstrate here that PMCAs protect PtdIns(4,5)P2 in the plasma membrane from hydrolysis by phospholipase C (PLC).. Comparison of active and inactive PMCAs indicates that the protection operates by two mechanisms; one requiring active PMCAs, the other not. Virus:Article Title: Peritoneal Tumorigenesis and Inflammation are Ameliorated by Humidified-Warm Carbon Dioxide Insufflation in the Mouse Article Snippet: .. 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Murine stem cell virus (MSCV)-mCherry-CT26 cells were obtained by stable retroviral transduction with an MSCV-mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV-IRES-GFP (Addgene) with the Article Title: Experimental study of delivery of humidified‐warm carbon dioxide during open abdominal surgery Article Snippet: .. Murine stem cell virus (MSCV)‐mCherry‐CT26 cells were generated by stable retroviral transduction with an MSCV‐mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV‐IRES‐GFP (Addgene, Cambridge, Massachusetts, USA) with the Transduction:Article Title: Peritoneal Tumorigenesis and Inflammation are Ameliorated by Humidified-Warm Carbon Dioxide Insufflation in the Mouse Article Snippet: .. Murine stem cell virus (MSCV)-mCherry-CT26 cells were obtained by stable retroviral transduction with an MSCV-mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV-IRES-GFP (Addgene) with the Article Title: Experimental study of delivery of humidified‐warm carbon dioxide during open abdominal surgery Article Snippet: .. Murine stem cell virus (MSCV)‐mCherry‐CT26 cells were generated by stable retroviral transduction with an MSCV‐mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV‐IRES‐GFP (Addgene, Cambridge, Massachusetts, USA) with the Plasmid Preparation:Article Title: Peritoneal Tumorigenesis and Inflammation are Ameliorated by Humidified-Warm Carbon Dioxide Insufflation in the Mouse Article Snippet: .. Murine stem cell virus (MSCV)-mCherry-CT26 cells were obtained by stable retroviral transduction with an MSCV-mCherry vector derived by replacing the green fluorescent protein (GFP) coding region of MSCV-IRES-GFP (Addgene) with the Article Title: Apart from its known function, the plasma membrane Ca²⁺ATPase can regulate Ca²⁺ signaling by controlling phosphatidylinositol 4,5-bisphosphate levels. Article Snippet: Plasma membrane Ca ATPases (PMCAs, also known as ATP2B1–ATP2B4) are known targets of phosphatidylinositol 4,5bisphosphate [PtdIns(4,5)P2], but if and how they control the PtdIns(4,5)P2 pool has not been considered.. We demonstrate here that PMCAs protect PtdIns(4,5)P2 in the plasma membrane from hydrolysis by phospholipase C (PLC).. Comparison of active and inactive PMCAs indicates that the protection operates by two mechanisms; one requiring active PMCAs, the other not. 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