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nontumorigenic breast epithelial cell line mcf12a  (ATCC)


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    ATCC nontumorigenic breast epithelial cell line mcf12a
    Nontumorigenic Breast Epithelial Cell Line Mcf12a, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf12a+cell+lines/MCF-12A/pmc12640659-180-53-59
    Average 96 stars, based on 297 article reviews
    nontumorigenic breast epithelial cell line mcf12a - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures.
    Article Snippet: .. 2D Cell Culture The MDA-MB-231, SKBR3 and MCF12A cell lines were purchased from the American Tissue Culture Collection (ATCC) and the MCF7 cell line was acquired from the European Collection of Authenticated Cell Cultures (ECACC). ..

    Article Title: Transgelin gene is frequently downregulated by promoter DNA hypermethylation in breast cancer.
    Article Snippet: .. Cell culture and AZA treatment of breast cell lines MCF7, MDA-MB-231, MDA-MB-453, MDA-MB-468, BT20, BT-474, ZR-75-1, HCC1937, MDA-MB-361, MDAMB-157, and MCF12A cell lines were purchased from American Type Culture Collection (ATCC). ..

    Multiple Displacement Amplification:

    Article Title: Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures.
    Article Snippet: .. 2D Cell Culture The MDA-MB-231, SKBR3 and MCF12A cell lines were purchased from the American Tissue Culture Collection (ATCC) and the MCF7 cell line was acquired from the European Collection of Authenticated Cell Cultures (ECACC). ..

    Article Title: Transgelin gene is frequently downregulated by promoter DNA hypermethylation in breast cancer.
    Article Snippet: .. Cell culture and AZA treatment of breast cell lines MCF7, MDA-MB-231, MDA-MB-453, MDA-MB-468, BT20, BT-474, ZR-75-1, HCC1937, MDA-MB-361, MDAMB-157, and MCF12A cell lines were purchased from American Type Culture Collection (ATCC). ..

    Article Title: The structure-function relationship of oncogenic LMTK3
    Article Snippet: .. ERα+ (MCF7, T47D), TNBC (MDA-MB-231, BT549) and non- transformed MCF12A cell lines were purchased from ATCC. .. MCF7 and MDA-MB-231 cells were maintained in low glucose DMEM (Sigma Aldrich, #D6046-500ML) supplemented with 10% FBS (Sigma Aldrich, #F7524-500ML) and 1% Penicillin/Streptomycin (Sigma-Aldrich, #P0781-100ML).

    Article Title: The neural stem cell gene PAFAH1B1 controls cell cycle progression, DNA integrity, and paclitaxel sensitivity of triple-negative breast cancer cells.
    Article Snippet: .. MDA-MB-231, HCC38, BT549, MCF10A, and MCF12A cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA). ..

    Article Title: The neural stem cell gene PAFAH1B1 controls cell cycle progression, DNA integrity, and paclitaxel sensitivity of triple-negative breast cancer cells
    Article Snippet: .. MDA-MB-231, HCC38, BT549, MCF10A, and MCF12A cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA). ..

    Article Title: Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures
    Article Snippet: .. The MDA-MB-231, SKBR3 and MCF12A cell lines were purchased from the American Tissue Culture Collection (ATCC) and the MCF7 cell line was acquired from the European Collection of Authenticated Cell Cultures (ECACC). ..

    Transformation Assay:

    Article Title: The structure-function relationship of oncogenic LMTK3
    Article Snippet: .. ERα+ (MCF7, T47D), TNBC (MDA-MB-231, BT549) and non- transformed MCF12A cell lines were purchased from ATCC. .. MCF7 and MDA-MB-231 cells were maintained in low glucose DMEM (Sigma Aldrich, #D6046-500ML) supplemented with 10% FBS (Sigma Aldrich, #F7524-500ML) and 1% Penicillin/Streptomycin (Sigma-Aldrich, #P0781-100ML).

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    mcf12a human non tumorigenic mammary epithelial cell line  (ATCC)
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    The percentage of non-tumorigenic <t>MCF12A</t> mammary cell viability with the most active compound 9 .
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    PAFAH1B1 silencing selectively reduces TNBC cell growth. A , TNBC cell lines MDA-MB-231, HCC38, and BT549 were transfected with either non-silencing control siRNA (siNS) or siRNA targeting PAFAH1B1 , and live cells were counted after 5 days using trypan blue exclusion or quantified using crystal violet staining. B , non-malignant breast cancer cell lines, MCF10A and <t>MCF12A,</t> were transfected and growth quantified after 5 days as in panel ( A ). Western blotting was performed at day 5 to confirm PAFAH1B1 silencing was maintained until the endpoint of the experiment. For all data, n = 3, points are technical replicates for each biological replicate, bars are means ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 by unpaired two-tailed t test.
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    cell line mcf12a  (ATCC)
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    PAFAH1B1 silencing selectively reduces TNBC cell growth. A , TNBC cell lines MDA-MB-231, HCC38, and BT549 were transfected with either non-silencing control siRNA (siNS) or siRNA targeting PAFAH1B1 , and live cells were counted after 5 days using trypan blue exclusion or quantified using crystal violet staining. B , non-malignant breast cancer cell lines, MCF10A and <t>MCF12A,</t> were transfected and growth quantified after 5 days as in panel ( A ). Western blotting was performed at day 5 to confirm PAFAH1B1 silencing was maintained until the endpoint of the experiment. For all data, n = 3, points are technical replicates for each biological replicate, bars are means ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 by unpaired two-tailed t test.
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    Image Search Results


    The percentage of non-tumorigenic MCF12A mammary cell viability with the most active compound 9 .

    Journal: Pharmaceuticals

    Article Title: Discovery and Evaluation of Novel Sulfonamide Derivatives Targeting Aromatase in ER+ Breast Cancer

    doi: 10.3390/ph18081206

    Figure Lengend Snippet: The percentage of non-tumorigenic MCF12A mammary cell viability with the most active compound 9 .

    Article Snippet: Adenocarcinomic human breast epithelial cells (MCF7, ATCC HTB-22), the mouse fibroblast cell line (NIH3T3, ATCC CRL-1658), and the MCF12A human non-tumorigenic mammary epithelial cell line were purchased from ATCC (Manassas, VA, USA).

    Techniques:

    PAFAH1B1 silencing selectively reduces TNBC cell growth. A , TNBC cell lines MDA-MB-231, HCC38, and BT549 were transfected with either non-silencing control siRNA (siNS) or siRNA targeting PAFAH1B1 , and live cells were counted after 5 days using trypan blue exclusion or quantified using crystal violet staining. B , non-malignant breast cancer cell lines, MCF10A and MCF12A, were transfected and growth quantified after 5 days as in panel ( A ). Western blotting was performed at day 5 to confirm PAFAH1B1 silencing was maintained until the endpoint of the experiment. For all data, n = 3, points are technical replicates for each biological replicate, bars are means ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 by unpaired two-tailed t test.

    Journal: The Journal of Biological Chemistry

    Article Title: The neural stem cell gene PAFAH1B1 controls cell cycle progression, DNA integrity, and paclitaxel sensitivity of triple-negative breast cancer cells

    doi: 10.1016/j.jbc.2025.110235

    Figure Lengend Snippet: PAFAH1B1 silencing selectively reduces TNBC cell growth. A , TNBC cell lines MDA-MB-231, HCC38, and BT549 were transfected with either non-silencing control siRNA (siNS) or siRNA targeting PAFAH1B1 , and live cells were counted after 5 days using trypan blue exclusion or quantified using crystal violet staining. B , non-malignant breast cancer cell lines, MCF10A and MCF12A, were transfected and growth quantified after 5 days as in panel ( A ). Western blotting was performed at day 5 to confirm PAFAH1B1 silencing was maintained until the endpoint of the experiment. For all data, n = 3, points are technical replicates for each biological replicate, bars are means ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 by unpaired two-tailed t test.

    Article Snippet: MDA-MB-231, HCC38, BT549, MCF10A, and MCF12A cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Transfection, Control, Staining, Western Blot, Two Tailed Test