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prey pact2 vector  (TaKaRa)


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    Structured Review

    TaKaRa prey pact2 vector
    Plasmids used in this study
    Prey Pact2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/matchmaker+pact2+vector/Human+Pancreas+Matchmaker+cDNA+Library+pACT2/pmc06433082-415-18-22
    Average 93 stars, based on 6 article reviews
    prey pact2 vector - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway"

    Article Title: N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA118.006913

    Plasmids used in this study
    Figure Legend Snippet: Plasmids used in this study

    Techniques Used: Plasmid Preparation, Marker

    Related Articles

    Transformation Assay:

    Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands
    Article Snippet: The DMM‐ HIS3 cassette‐containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3‐52, his3‐200, ade2‐101, lys2‐801, leu2‐3, 112, trp1‐901, tyr1‐501, gal4‐D512, gal80‐D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Matchmaker pACT2 vector (Clontech). ..

    Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands
    Article Snippet: The DMM-HIS3 cassette-containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4D512, gal80-D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Matchmaker pACT2 vector (Clontech). ..

    Activation Assay:

    Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands
    Article Snippet: The DMM‐ HIS3 cassette‐containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3‐52, his3‐200, ade2‐101, lys2‐801, leu2‐3, 112, trp1‐901, tyr1‐501, gal4‐D512, gal80‐D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Matchmaker pACT2 vector (Clontech). ..

    Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands
    Article Snippet: The DMM-HIS3 cassette-containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4D512, gal80-D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Matchmaker pACT2 vector (Clontech). ..

    Plasmid Preparation:

    Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands
    Article Snippet: The DMM‐ HIS3 cassette‐containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3‐52, his3‐200, ade2‐101, lys2‐801, leu2‐3, 112, trp1‐901, tyr1‐501, gal4‐D512, gal80‐D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Matchmaker pACT2 vector (Clontech). ..

    Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands
    Article Snippet: The DMM-HIS3 cassette-containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4D512, gal80-D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Matchmaker pACT2 vector (Clontech). ..



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    Image Search Results


    Plasmids used in this study

    Journal: The Journal of Biological Chemistry

    Article Title: N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway

    doi: 10.1074/jbc.RA118.006913

    Figure Lengend Snippet: Plasmids used in this study

    Article Snippet: Y2H screening and binding assays To screen human UBR1-binding proteins, a human pancreas cDNA library cloned into the prey pACT2 vector (638820, Clontech) was transformed into S. cerevisiae strain pJ69 l4A expressing a bait pCH1591 (expressing human UBR1 1–1031 ) using the lithium-acetate protocol ( 61 ).

    Techniques: Plasmid Preparation, Marker