prey pact2 vector (TaKaRa)
Structured Review

Prey Pact2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/matchmaker+pact2+vector/Human+Pancreas+Matchmaker+cDNA+Library+pACT2/pmc06433082-415-18-22
Average 93 stars, based on 6 article reviews
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1) Product Images from "N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway"
Article Title: N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.RA118.006913
Figure Legend Snippet: Plasmids used in this study
Techniques Used: Plasmid Preparation, Marker
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Transformation Assay:Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands Article Snippet: The DMM‐ HIS3 cassette‐containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3‐52, his3‐200, ade2‐101, lys2‐801, leu2‐3, 112, trp1‐901, tyr1‐501, gal4‐D512, gal80‐D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands Article Snippet: The DMM-HIS3 cassette-containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4D512, gal80-D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Activation Assay:Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands Article Snippet: The DMM‐ HIS3 cassette‐containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3‐52, his3‐200, ade2‐101, lys2‐801, leu2‐3, 112, trp1‐901, tyr1‐501, gal4‐D512, gal80‐D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands Article Snippet: The DMM-HIS3 cassette-containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4D512, gal80-D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Plasmid Preparation:Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands Article Snippet: The DMM‐ HIS3 cassette‐containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3‐52, his3‐200, ade2‐101, lys2‐801, leu2‐3, 112, trp1‐901, tyr1‐501, gal4‐D512, gal80‐D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the Article Title: Liver X Receptor–Binding DNA Motif Associated With Atherosclerosis‐Specific DNA Methylation Profiles of Alu Elements and Neighboring CpG Islands Article Snippet: The DMM-HIS3 cassette-containing fragment generated by ApaI digestion was inserted into the genome of the Saccharomyces cerevisiae YM4271 strain (genotype: MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4D512, gal80-D538, ade5::hisG) to generate the bait strain. .. The latter was transformed using 0.5 mg of a Gal4 activation domain (AD)–human aorta cDNA fusion library (prey) in the |