Journal: BMC Genomics

Article Title: Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples

doi: 10.1186/1471-2164-13-16

Figure Lengend Snippet: Comparison of allele frequencies 1 estimated using high-throughput sequencing vs. genotyping for 43 SNPs across two pools of 75 dairy cattle divergent for calving interval (CIV) . 1 Actual genotype frequencies calculated from Sequenom ® MassARRAY data obtained from previous studies [ , , , , ].

Article Snippet: Estimated allele frequencies of a selection of SNPs from the sequence capture target enrichment and sequencing of pooled samples were compared to actual allele frequencies generated using Sequenom ® MassARRAY iPLEXTM gold assay.

Techniques: Comparison, Next-Generation Sequencing

Journal: BMC Genomics

Article Title: Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples

doi: 10.1186/1471-2164-13-16

Figure Lengend Snippet: Comparison between allele frequencies generated using Sequenom ® MassARRAY and Illumina GAIIx technologies.

Article Snippet: Estimated allele frequencies of a selection of SNPs from the sequence capture target enrichment and sequencing of pooled samples were compared to actual allele frequencies generated using Sequenom ® MassARRAY iPLEXTM gold assay.

Techniques: Comparison, Generated

Journal: BMC Genetics

Article Title: DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding ( GNAS ) genomic imprinting domain are associated with performance traits

doi: 10.1186/1471-2156-12-4

Figure Lengend Snippet: Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.

Article Snippet: All SNP genotyping was performed commercially by Sequenom Inc. (San Diego, CA, USA; http://www.sequenom.com using their proprietary MassARRAY iPLEXTM Gold genotyping platform.

Techniques: Sequencing, Expressing