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Matrix Science mascot database search software
Mascot Database Search Software, supplied by Matrix Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mascot+database+search+software/mascot+software/10__1093_slash_jimmun_slash_vkae019-136-16-22
Average 90 stars, based on 1 article reviews
mascot database search software - by Bioz Stars, 2026-09
90/100 stars

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Tandem Mass Spectroscopy:

Article Title: REEP1 Preserves Motor Function in SOD1 G93A Mice by Improving Mitochondrial Function via Interaction with NDUFA4.
Article Snippet: A decline in the activities of oxidative phosphorylation (OXPHOS) complexes has been consistently reported in amyotrophic lateral sclerosis (ALS) patients and animal models of ALS, although the underlying molecular mechanisms are still elusive.. Here, we report that receptor expression enhancing protein 1 (REEP1) acts as an important regulator of complex IV assembly, which is pivotal to preserving motor neurons in SOD1G93A mice.. We found the expression of REEP1 was greatly reduced in transgenic SOD1G93A mice with ALS.

Article Title: Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.
Article Snippet: Edited by Michael Ibba Mutations in PINK1 (PTEN-induced putative kinase 1) are associated with autosomal recessive early-onset Parkinson’s disease.. Full-length PINK1 (PINK1-l) has been extensively studied in mitophagy; however, the functions of the short form of PINK1 (PINK1-s) remain poorly understood.. Here, we report that PINK1-s is recruited to ribosome fractions after short-term inhibition of proteasomes.

Article Title: Methylene blue decreases mitochondrial lysine acetylation in the diabetic heart
Article Snippet: Lysine-acetylated peptides were enriched by immunoprecipitation with anti-Ac-Lys antibody beads (ImmuneChem Pharmaceuticals), and analyzed by LC-MS/MS using an UltiMate 3000 LC system (Dionex Inc., Sunnyvale, California) interfaced with a Velos Pro Ion Trap/Orbitrap Elite hybrid mass spectrometer (Thermo Scientific, Bremen, Germany) [ ]. .. Lysine-acetylated peptides were identified by comparing resultant MS/MS spectra against the Swiss-Prot database using Mascot database search software (version 2.4, Matrix Science, London, UK). ..

Article Title: Aggregatin is a mitochondrial regulator of MAVS activation to drive innate immunity
Article Snippet: Mitochondrial antiviral-signaling protein (MAVS) is a key adapter protein required for inducing type I interferons (IFN-Is) and other antiviral effector molecules.. The formation of MAVS aggregates on mitochondria is essential for its activation; however, the regulatory mitochondrial factor that mediates the aggregation process is unknown.. Our recent work has identified the protein Aggregatin as a critical seeding factor for β-amyloid peptide aggregation.

Software:

Article Title: REEP1 Preserves Motor Function in SOD1 G93A Mice by Improving Mitochondrial Function via Interaction with NDUFA4.
Article Snippet: A decline in the activities of oxidative phosphorylation (OXPHOS) complexes has been consistently reported in amyotrophic lateral sclerosis (ALS) patients and animal models of ALS, although the underlying molecular mechanisms are still elusive.. Here, we report that receptor expression enhancing protein 1 (REEP1) acts as an important regulator of complex IV assembly, which is pivotal to preserving motor neurons in SOD1G93A mice.. We found the expression of REEP1 was greatly reduced in transgenic SOD1G93A mice with ALS.

Article Title: Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.
Article Snippet: Edited by Michael Ibba Mutations in PINK1 (PTEN-induced putative kinase 1) are associated with autosomal recessive early-onset Parkinson’s disease.. Full-length PINK1 (PINK1-l) has been extensively studied in mitophagy; however, the functions of the short form of PINK1 (PINK1-s) remain poorly understood.. Here, we report that PINK1-s is recruited to ribosome fractions after short-term inhibition of proteasomes.

Article Title: Biochemical characterization of plasmepsin V from Plasmodium vivax Thailand isolates: Substrate specificity and enzyme inhibition.
Article Snippet: Plasmepsin V (PMV) is a Plasmodium aspartic protease responsible for the cleavage of the Plasmodium export element (PEXEL) motif, which is an essential step for export of PEXEL containing proteins and crucial for parasite viability.. Here we describe the genetic polymorphism of Plasmodium vivax PMV (PvPMV) Thailand isolates, followed by cloning, expression, purification and characterization of PvPMVThai, presenting the proand mature-form of PvPMV-Thai.. With our refolding and purification method, approximately 1 mg of PvPMV-Thai was obtained from 1 g of washed inclusion bodies.

Article Title: Methylene blue decreases mitochondrial lysine acetylation in the diabetic heart
Article Snippet: Lysine-acetylated peptides were enriched by immunoprecipitation with anti-Ac-Lys antibody beads (ImmuneChem Pharmaceuticals), and analyzed by LC-MS/MS using an UltiMate 3000 LC system (Dionex Inc., Sunnyvale, California) interfaced with a Velos Pro Ion Trap/Orbitrap Elite hybrid mass spectrometer (Thermo Scientific, Bremen, Germany) [ ]. .. Lysine-acetylated peptides were identified by comparing resultant MS/MS spectra against the Swiss-Prot database using Mascot database search software (version 2.4, Matrix Science, London, UK). ..

Article Title: Aggregatin is a mitochondrial regulator of MAVS activation to drive innate immunity
Article Snippet: Mitochondrial antiviral-signaling protein (MAVS) is a key adapter protein required for inducing type I interferons (IFN-Is) and other antiviral effector molecules.. The formation of MAVS aggregates on mitochondria is essential for its activation; however, the regulatory mitochondrial factor that mediates the aggregation process is unknown.. Our recent work has identified the protein Aggregatin as a critical seeding factor for β-amyloid peptide aggregation.

Article Title: Comparative Analysis of the Ultrastructure Between Discoid and Normal-Shaped Lateral Meniscus
Article Snippet: The EASY-Spray column (75 μm, 120 mm length, packed with C-18 resin, Thermo) was used as the analytical column. .. MS raw data files were extracted by using the ProteoWizard software (version 3.0.8789) and searched against the Swiss-Prot human protein sequence database (version 57) to identify distinct peptides and proteins via the Mascot database search software (version 2.1.04, Matrix Science, London, UK). ..

Article Title: Plasmodium falciparum Plasmepsin V (PfPMV): Insights into recombinant expression, substrate specificity and active site structure.
Article Snippet: Plasmepsin V from Plasmodium falciparum (PfPMV) is responsible for the cleavage of the Plasmodium export element (PEXEL) motif at the N-terminus of several hundreds of the exported proteins.. PfPMV is necessary for parasite viability and has become a novel promising target for antimalarial therapy.. The first recombinant expression of soluble, active PfPMV as thioredoxin fusion proteins is reported herein.

Article Title: Monitoring the synthesis of biomolecules using mass spectrometry
Article Snippet: .. Mascot database search software (Matrix Science, London, UK) for proteins and METLIN ( http://metlin.scripps.edu ) for metabolites. ..

Sequencing:

Article Title: Comparative Analysis of the Ultrastructure Between Discoid and Normal-Shaped Lateral Meniscus
Article Snippet: The EASY-Spray column (75 μm, 120 mm length, packed with C-18 resin, Thermo) was used as the analytical column. .. MS raw data files were extracted by using the ProteoWizard software (version 3.0.8789) and searched against the Swiss-Prot human protein sequence database (version 57) to identify distinct peptides and proteins via the Mascot database search software (version 2.1.04, Matrix Science, London, UK). ..



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Image Search Results


Journal: Cell reports

Article Title: MYO10 regulates genome stability and cancer inflammation through mediating mitosis

doi: 10.1016/j.celrep.2023.112531

Figure Lengend Snippet:

Article Snippet: Phosphorylated peptides were identified by comparing all the experimental peptide MS/MS spectra against the amino acid sequence of MYO10 using Mascot database search software (version 2.4, Matrix Science, London, UK).

Techniques: Staining, Recombinant, SYBR Green Assay, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Knock-Out, Software, Fluorescence, Microscopy