product references rat anti mac 2 mab cedarlane  (Cedarlane)

Journal: PLoS ONE

Article Title: Efficacy and Mechanism of Angiotensin II Receptor Blocker Treatment in Experimental Abdominal Aortic Aneurysms

doi: 10.1371/journal.pone.0049642

Figure Lengend Snippet: After sacrifice, aortae were fixed, sectioned and underwent elastin staining and immunostaining for SMCs (SMC α-actin), macrophages (MAC2) and blood vessels (CD31). A–K: Residual medial elastin (A–E) and mural SMCs (F–K) in telmisartan (B, H), irbesartan (C, I), fluvastatin (D, J), doxycycline-treated (E, K) or control mice (A, F). L–V: macrophages (L–P) and endothelial cells (neovessels) (R–V) in telmisartan- (M, S), irbesartan- (N, T), fluvastatin- (O, U) or doxycycline- (P, V) treated mice or untreated control mice (L, R). Representative images, 4–5 mice in each group. Magnification: x200 in A–E, x100 in F–V.

Article Snippet: In brief, the PBS-rehydrated sections were incubated with a rabbit anti-mouse SMC α-actin polyclonal antibody (Laboratory Vision, Fremont, CA), a rat anti-mouse MAC2 mAb (M3/38, Cedarlane Laboratories, Burlington, Ontario, Canada), a rabbit anti-mouse CD31 polyclonal antibody (Laboratory Vision, Fremont, CA), or a species and isotype-matched negative control antibody.

Techniques: Staining, Immunostaining

Journal: Cell stem cell

Article Title: Smooth muscle cell reprogramming in aortic aneurysms

doi: 10.1016/j.stem.2020.02.013

Figure Lengend Snippet: (A) Histologic analysis of mouse ascending aortas dissected from 24-week-old Apoe−/− and TGFβR2iSMC-Apoe mice after 4 months of high cholesterol high fat diet (HCHFD). Representative low-magnification images of Oil-Red-O (left) and Alcian Blue (stain mucopolysaccharides sialomucins for cartilage)/Von Kossa (stain calcium for bone) (right) stained mouse ascending aortas. N=6 mice/group. Scale bar: 200 μm. (B) Representative images of H&E, Elastin, Oil-Red-O, Safranin O (cartilage)/Fast Green, Alcian Blue (stain mucopolysaccharides sialomucins for cartilage)/Von Kossa (stain calcium for bone), and Alizarin Red (calcium)-stained mouse ascending aortas from Apoe−/− and TGFβR2iSMC-Apoe mice (N=6 mice/group). Scale bar: 50 μm. (C) Representative IMC images stained for GFP (green), MYH11 (yellow), Aggrecan (cyan), Osteopontin (red), Adiponectin (blue), and Mac-2 (magenta) from Apoe−/− and TGFβR2iSMC-Apoe ascending aorta are shown overlaid (N=6 mice/group). Scale bar: 500 μm. (D) A high-magnification of (C) showing separate channels of GFP (green), MYH11 (red), Aggrecan (red), Osteopontin (red), and Adiponectin (red). (E) Segmentation of individual cell membrane using CellProlifer. (F) The segmentation mask in (E) was used as the basis for analysis of raw data from this IMC experiment. Based on raw data from each of the four input channels, each individual cell was clustered by expression by histoCAT and manually assigned a phenotype. PhenoGraph clustering of all cell phenotype visualized as a distinct color on the tSNE plot. (G) Heatmap visualizing the marker intensity for each Phenograph. See also Figure S3 and Table S2A.

Article Snippet: Rabbit monoclonal anti-MAC-2 (clone D4I2R) , Cell Signaling Technology , Cat# 87985, RRID:AB_2800111.

Techniques: Staining, Expressing, Marker

Journal: Cell stem cell

Article Title: Smooth muscle cell reprogramming in aortic aneurysms

doi: 10.1016/j.stem.2020.02.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-MAC-2 (clone D4I2R) , Cell Signaling Technology , Cat# 87985, RRID:AB_2800111.

Techniques: Recombinant, Multiplex Assay, Staining, In Situ, Marker, Expressing, Plasmid Preparation, shRNA, Software