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ma 104 cells  (ATCC)


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    ATCC ma 104 cells
    Ma 104 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ma+104+cells/MA-104+Clone+1/pm42906372-50-5-9
    Average 96 stars, based on 383 article reviews
    ma 104 cells - by Bioz Stars, 2026-11
    96/100 stars

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    Modification:

    Article Title: Preparation of a new monoclonal antibody against D205R protein of African swine fever virus and identification of its linear antigenic epitope.
    Article Snippet: African swine fever virus (ASFV), a highly contagious virus with a double-stranded DNA genome, is notorious for causing severe hemorrhagic fever in pigs, often leading to mortality rates as high as 100 %.. First identified in Kenya in 1921, the virus has since spread globally, with a significant outbreak in China in 2018, causing extensive economic losses in the swine industry.. The D205R protein (pD205R) of ASFV, classified as a nonstructural protein, plays a role in the transcription of viral genes and is associated with ASFV RNA polymerase.

    Article Title: Strong and early monkeypox virus-specific immunity associated with mild disease after intradermal clade-IIb-infection in CAST/EiJ-mice.
    Article Snippet: .. MA-104 cells (ATCC® CRL-2378.1TM) and Vero E6 cells (ATCC® CRL1586TM) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS, 1% non-essential amino acids (only for MA-104 cells), and 1% penicillin-streptomycin. .. MPXV clade IIa (isolate West Africa/Taï National Park/ Côte d’Ivoire/ 2012), and MPXV clade IIb (isolate muc IMB-1/Germany/2022) were propagated on MA-104 cells in DMEM (Sigma-Aldrich) supplemented with 2% FBS, 1% non-essential amino acids, and 1% penicillinstreptomycin at 37 °C.

    Cell Culture:

    Article Title: Strong and early monkeypox virus-specific immunity associated with mild disease after intradermal clade-IIb-infection in CAST/EiJ-mice.
    Article Snippet: .. MA-104 cells (ATCC® CRL-2378.1TM) and Vero E6 cells (ATCC® CRL1586TM) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS, 1% non-essential amino acids (only for MA-104 cells), and 1% penicillin-streptomycin. .. MPXV clade IIa (isolate West Africa/Taï National Park/ Côte d’Ivoire/ 2012), and MPXV clade IIb (isolate muc IMB-1/Germany/2022) were propagated on MA-104 cells in DMEM (Sigma-Aldrich) supplemented with 2% FBS, 1% non-essential amino acids, and 1% penicillinstreptomycin at 37 °C.

    Article Title: Hairpin loop to hairpin loop: a full-length assembly of the ASFV genome using Oxford Nanopore long-read sequencing
    Article Snippet: Aiming to get a full-length ASFV genome, we employed Oxford Nanopore Technologies (ONT) to sequence the genome of a cell culture-adapted ASFV originally isolated from infected Large White pigs in Phanat Nikhom District, Chonburi, Thailand in August 2023. .. The isolated ASFV was cultured in MA-104 cells (ATCC CRL-2378.1) in DMEM (Cytiva; Cat# SH30243.02) with 10% fetal bovine serum (FBS; Sigma; Cat# F7524) and was serially passaged until passage 50. ..

    other:

    Article Title: Loss of nsp14-exonuclease activity impairs the replication, proofreading, fitness and pathogenesis of SARS-CoV-2
    Article Snippet: Cells were maintained at 37°C, 5%CO 2 , and detached during passage and expansion with 0.05% trypsin-EDTA (Gibco).

    Virus:

    Article Title: Early treatment with tecovirimat against mpox disease in cynomolgus macaques.
    Article Snippet: All animals were infected intravenously with 1 × 106 TCID50 mpox clade IIb virus (hMPXV/USA/MA001/ 2022; lineage B.1, Clade IIb), originally sourced from BEI Resources (NR-58622). .. The virus was propagated on MA-104 cells (Clone 1; ATCC CRL 2378.1; RRID: CVCL_3846) to generate working stocks at Bioqual, Inc., and titre was determined by plaque assay on Vero E6 cells (ATCC CRL-1586/Vero C1008; RRID: CVCL_0574). ..

    Plaque Assay:

    Article Title: Early treatment with tecovirimat against mpox disease in cynomolgus macaques.
    Article Snippet: All animals were infected intravenously with 1 × 106 TCID50 mpox clade IIb virus (hMPXV/USA/MA001/ 2022; lineage B.1, Clade IIb), originally sourced from BEI Resources (NR-58622). .. The virus was propagated on MA-104 cells (Clone 1; ATCC CRL 2378.1; RRID: CVCL_3846) to generate working stocks at Bioqual, Inc., and titre was determined by plaque assay on Vero E6 cells (ATCC CRL-1586/Vero C1008; RRID: CVCL_0574). ..

    Isolation:

    Article Title: Hairpin loop to hairpin loop: a full-length assembly of the ASFV genome using Oxford Nanopore long-read sequencing
    Article Snippet: Aiming to get a full-length ASFV genome, we employed Oxford Nanopore Technologies (ONT) to sequence the genome of a cell culture-adapted ASFV originally isolated from infected Large White pigs in Phanat Nikhom District, Chonburi, Thailand in August 2023. .. The isolated ASFV was cultured in MA-104 cells (ATCC CRL-2378.1) in DMEM (Cytiva; Cat# SH30243.02) with 10% fetal bovine serum (FBS; Sigma; Cat# F7524) and was serially passaged until passage 50. ..



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    (A-B) HEK293T cells were co-transfected with mCherry-pSTING (20 ng) or its mutants together with pcGAS-HA (20 ng), plus ISRE-luc (10 ng) or NF-κΒ-luc (10 ng) and pRL-TK plasmid (0.4 ng) for 24 h. Luciferase activities were detected, and protein expressions were analyzed. (C-F) 3D4/21 cells in 24-well cell crawl slides were co-transfected with Flag-pSTING or K61R mutant together with KDEL-Red (C), mApple-SiT (D), MITO-GFP (E) or Lamp1-GFP (F) (each 0.5 μg) for 24 h, and then cells were fixed, stained and the colocalization of STING and its K61R mutant with organelles was examined by confocal fluorescence microscopy. (G) HEK293T cells in 6-well were transfected with Flag-pSTING or K61R mutant (1 μg/mL) for 24h, and stimulated with 2’3’-cGAMP (2 μg/mL) for 2 h. Then interaction between STING and TBK1/IRF3 was identified by Co-IP using anti-FLAG antibody, whereas rabbit IgG served as the control. (H-I) STING -/- 3D4/21 cells in 12-well were transfected with pmCherry-C1, mCherry-pSTING or K61R mutant (1 μg) for 24 h, and stimulated with 2’3’-cGAMP (2 μg/mL) for indicated times (H) or 6 h (I). Cells were harvested and analyzed by Western blotting (H) and RT-qPCR (I). (J-M) STING -/- 3D4/21 cells were transfected with Flag-pSTING or its mutants (1μg), and then stimulated with 2’3’-cGAMP (2 μg/mL) for 2 h, followed by infections with HSV-1 (MOI=0.01, 36 h) or PRV (MOI=0.01, 24 h). The viral titers in the supernatants from HSV-1 (J) or PRV (L) infected cells was measured by plaque assay, and the HSV-1 and PRV gD gene expressions were measured by RT-qPCR (K and M). ( N-P ) STING -/- <t>MA104</t> cells were transfected and stimulated as in STING -/- 3D4/21 cells, followed by infections ASFV-GFP, infected cells were detected by fluorescence microscopy and cytometry, Western blotting and quantitative PCR. * p < 0.05 and ** p < 0.01.
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    ma104 cell - by Bioz Stars, 2026-11
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    		  Buy from Supplier

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    (A-B) HEK293T cells were co-transfected with mCherry-pSTING (20 ng) or its mutants together with pcGAS-HA (20 ng), plus ISRE-luc (10 ng) or NF-κΒ-luc (10 ng) and pRL-TK plasmid (0.4 ng) for 24 h. Luciferase activities were detected, and protein expressions were analyzed. (C-F) 3D4/21 cells in 24-well cell crawl slides were co-transfected with Flag-pSTING or K61R mutant together with KDEL-Red (C), mApple-SiT (D), MITO-GFP (E) or Lamp1-GFP (F) (each 0.5 μg) for 24 h, and then cells were fixed, stained and the colocalization of STING and its K61R mutant with organelles was examined by confocal fluorescence microscopy. (G) HEK293T cells in 6-well were transfected with Flag-pSTING or K61R mutant (1 μg/mL) for 24h, and stimulated with 2’3’-cGAMP (2 μg/mL) for 2 h. Then interaction between STING and TBK1/IRF3 was identified by Co-IP using anti-FLAG antibody, whereas rabbit IgG served as the control. (H-I) STING -/- 3D4/21 cells in 12-well were transfected with pmCherry-C1, mCherry-pSTING or K61R mutant (1 μg) for 24 h, and stimulated with 2’3’-cGAMP (2 μg/mL) for indicated times (H) or 6 h (I). Cells were harvested and analyzed by Western blotting (H) and RT-qPCR (I). (J-M) STING -/- 3D4/21 cells were transfected with Flag-pSTING or its mutants (1μg), and then stimulated with 2’3’-cGAMP (2 μg/mL) for 2 h, followed by infections with HSV-1 (MOI=0.01, 36 h) or PRV (MOI=0.01, 24 h). The viral titers in the supernatants from HSV-1 (J) or PRV (L) infected cells was measured by plaque assay, and the HSV-1 and PRV gD gene expressions were measured by RT-qPCR (K and M). ( N-P ) STING -/- MA104 cells were transfected and stimulated as in STING -/- 3D4/21 cells, followed by infections ASFV-GFP, infected cells were detected by fluorescence microscopy and cytometry, Western blotting and quantitative PCR. * p < 0.05 and ** p < 0.01.

    Journal: bioRxiv

    Article Title: Species-specific regulation of porcine STING stability and antiviral signaling via its K61 mediated K48 ubiquitination and proteasome degradation

    doi: 10.64898/2026.03.26.714395

    Figure Lengend Snippet: (A-B) HEK293T cells were co-transfected with mCherry-pSTING (20 ng) or its mutants together with pcGAS-HA (20 ng), plus ISRE-luc (10 ng) or NF-κΒ-luc (10 ng) and pRL-TK plasmid (0.4 ng) for 24 h. Luciferase activities were detected, and protein expressions were analyzed. (C-F) 3D4/21 cells in 24-well cell crawl slides were co-transfected with Flag-pSTING or K61R mutant together with KDEL-Red (C), mApple-SiT (D), MITO-GFP (E) or Lamp1-GFP (F) (each 0.5 μg) for 24 h, and then cells were fixed, stained and the colocalization of STING and its K61R mutant with organelles was examined by confocal fluorescence microscopy. (G) HEK293T cells in 6-well were transfected with Flag-pSTING or K61R mutant (1 μg/mL) for 24h, and stimulated with 2’3’-cGAMP (2 μg/mL) for 2 h. Then interaction between STING and TBK1/IRF3 was identified by Co-IP using anti-FLAG antibody, whereas rabbit IgG served as the control. (H-I) STING -/- 3D4/21 cells in 12-well were transfected with pmCherry-C1, mCherry-pSTING or K61R mutant (1 μg) for 24 h, and stimulated with 2’3’-cGAMP (2 μg/mL) for indicated times (H) or 6 h (I). Cells were harvested and analyzed by Western blotting (H) and RT-qPCR (I). (J-M) STING -/- 3D4/21 cells were transfected with Flag-pSTING or its mutants (1μg), and then stimulated with 2’3’-cGAMP (2 μg/mL) for 2 h, followed by infections with HSV-1 (MOI=0.01, 36 h) or PRV (MOI=0.01, 24 h). The viral titers in the supernatants from HSV-1 (J) or PRV (L) infected cells was measured by plaque assay, and the HSV-1 and PRV gD gene expressions were measured by RT-qPCR (K and M). ( N-P ) STING -/- MA104 cells were transfected and stimulated as in STING -/- 3D4/21 cells, followed by infections ASFV-GFP, infected cells were detected by fluorescence microscopy and cytometry, Western blotting and quantitative PCR. * p < 0.05 and ** p < 0.01.

    Article Snippet: HEK293T cells (ATCC cat # CRL-3216), Vero cells (ATCC cat # CCL-81), Raw264.7 cells (ATCC cat # TIB-71), MDBK cells (ATCC cat # CCL-22) and MA104 cell (ATCC cat # CRL-2378.1) were cultured in DMEM (Hyclone Laboratories, USA) containing 10% fetal bovine serum (FBS, Vazyme Biotech Co., Ltd) and 100 IU/ml of penicillin plus 100 μg/ml streptomycin.

    Techniques: Transfection, Plasmid Preparation, Luciferase, Mutagenesis, Staining, Fluorescence, Microscopy, Co-Immunoprecipitation Assay, Control, Western Blot, Quantitative RT-PCR, Infection, Plaque Assay, Cytometry, Real-time Polymerase Chain Reaction