Review

m14 cells (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC m14 cells
M14 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/M1%2E4/10__5114_slash_aoms_slash_209585-36-2-4
Average 95 stars, based on 356 article reviews

m14 cells - by Bioz Stars, 2026-09

95/100 stars

Images

Cell Culture:

Article Title: Synthesis and Biological Evaluation of Cyclobutane-Based β3 Integrin Antagonists: A Novel Approach to Targeting Integrins for Cancer Therapy
Article Snippet: RGDS was obtained from Sigma-Aldrich, cRGDfV from Enzo Life Sciences (Farmingdale, NY, USA), and GR144053 from Tocris Bioscience (Bristol, UK). .. Human melanoma SK-Mel-2 and M14 cells (ATCC, LGC Standards, Teddington, UK) were cultured in RPMI 1640 cell culture medium supplemented with 10% FBS, 1 mM sodium pyruvate and 2 mM L-glutamine (all Sigma) at 37 °C in a humidified atmosphere containing 5% CO 2 . ..

Article Title: Integrated Genomics Identifies miR-181/TFAM Pathway as a Critical Driver of Drug Resistance in Melanoma
Article Snippet: .. A375, M14 cells were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured RPMI 1640 (Invitrogen, Karlsruhe, Germany) with L-Glutamine, 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 100 U/mL penicillin and 50 μg of streptomycin, at 37 °C in humidified 5% CO 2 atmosphere. .. HeLa cells (No. CCL 185; American Type Culture Collection, Rockville, MD, USA) were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamine (GIBCO BRL, Life Technologies) at 37°C and 5% CO2.

Article Title: Synthesis and Biological Evaluation of Cyclobutane-Based β3 Integrin Antagonists: A Novel Approach to Targeting Integrins for Cancer Therapy.
Article Snippet: RGDS was obtained from Sigma-Aldrich, cRGDfV from Enzo Life Sciences (Farmingdale, NY, USA), and GR144053 from Tocris Bioscience (Bristol, UK). .. Human melanoma SK-Mel-2 and M14 cells (ATCC, LGC Standards, Teddington, UK) were cultured in RPMI 1640 cell culture medium supplemented with 10% FBS, 1 mM sodium pyruvate and 2 mM L-glutamine (all Sigma) at 37 ◦C in a humidified atmosphere containing 5% CO2. ..

other:

Article Title: Registered report: Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors
Article Snippet: M14 cells , Cells , ATCC , HTB-129 , Original source of the cells unspecified.

Expressing:

Article Title: Rsf-1 regulates malignant melanoma cell viability and chemoresistance via NF-κB/Bcl-2 signaling
Article Snippet: .. M14 cells with low Rsf-1 expression, and MV3 and A375 cells with high Rsf-1 expression were purchased from the American Type Culture Collection (Manassas, VA, USA). .. M14 and A375 cells were cultivated in Dulbecco's Modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc.).

Similar Products

m14 cells (ATCC)

ATCC m14 cells
M14 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/M1%2E4/10__5114_slash_aoms_slash_209585-36-2-4
Average 95 stars, based on 1 article reviews

m14 cells - by Bioz Stars, 2026-09

95/100 stars

Buy from Supplier

melanoma m14 6 48 leukemia hl 60 tb 6 44 non small cell lung cancer hop 62 (ATCC)

ATCC melanoma m14 6 48 leukemia hl 60 tb 6 44 non small cell lung cancer hop 62
Melanoma M14 6 48 Leukemia Hl 60 Tb 6 44 Non Small Cell Lung Cancer Hop 62, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/M1%2E4/pm23899273__jo401171q_si_001-16-141-176
Average 95 stars, based on 1 article reviews

melanoma m14 6 48 leukemia hl 60 tb 6 44 non small cell lung cancer hop 62 - by Bioz Stars, 2026-09

95/100 stars

Buy from Supplier

m14 cell lines (ATCC)

ATCC m14 cell lines
M14 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/M1%2E4/pm40763661-474-4-10
Average 95 stars, based on 1 article reviews

m14 cell lines - by Bioz Stars, 2026-09

95/100 stars

Buy from Supplier

leukemia hl 60 tb 6 39 non small cell lung cancer nci h460 6 37 melanoma m14 6 34 breast cancer mcf7 6 34 cns cancer u251 (ATCC)

ATCC leukemia hl 60 tb 6 39 non small cell lung cancer nci h460 6 37 melanoma m14 6 34 breast cancer mcf7 6 34 cns cancer u251
Leukemia Hl 60 Tb 6 39 Non Small Cell Lung Cancer Nci H460 6 37 Melanoma M14 6 34 Breast Cancer Mcf7 6 34 Cns Cancer U251, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/MCF7/pmc11702208__RA___015___D4RA07174C___s001-10-47-89
Average 99 stars, based on 1 article reviews

leukemia hl 60 tb 6 39 non small cell lung cancer nci h460 6 37 melanoma m14 6 34 breast cancer mcf7 6 34 cns cancer u251 - by Bioz Stars, 2026-09

99/100 stars

Buy from Supplier

human metastatic melanoma cell line m14 (Procell Inc)

Procell Inc human metastatic melanoma cell line m14

Identification of differentially expressed genes based on ImmuneScore and StromalScore. ( A-C ) Overall survival analysis of high and low Immune/Stromal/ESTIMATE Scores in patients with <t>metastatic</t> melanoma using the ESTIMATE algorithm. ( D , E ) Heatmap depicting the top 100 DEmRNAs with the most significant p-values between high and low Immune/Stromal Scores (|log Fold change|>1.9, p < 0.001). Blue represents downregulation, while red represents upregulation. ( F ) Venn diagram showing the upregulated and downregulated genes commonly intersecting between Stromal and Immune Scores, with green indicating immune-related DEmRNAs and purple indicating stromal-related DEmRNAs. ( G ) Construction of a protein-protein interaction network for overlapping genes using the STRING online tool (confidence score > 0.900) and Cytoscape software. ( H ) The top 15 genes ranked by nodes in the PPI network are shown in the graph. ( I ) Univariate Cox analysis was conducted on genes with a node count of 15 or more in the PPI network to identify differentially expressed genes significantly associated with prognosis

Human Metastatic Melanoma Cell Line M14, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/the+human+a2058+cell+line/pmc11682626-61-8-18
Average 90 stars, based on 1 article reviews

human metastatic melanoma cell line m14 - by Bioz Stars, 2026-09

90/100 stars

Buy from Supplier

representative melanoma cell line m14 (ATCC)

ATCC representative melanoma cell line m14

Representative Melanoma Cell Line M14, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/M1%2E4/pmc10069597-41-0-16
Average 95 stars, based on 1 article reviews

representative melanoma cell line m14 - by Bioz Stars, 2026-09

95/100 stars

Buy from Supplier

m14 cells (iCell Bioscience Inc)

iCell Bioscience Inc m14 cells

M14 Cells, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/m14+cells/pm36323090-43-12-14
Average 90 stars, based on 1 article reviews

m14 cells - by Bioz Stars, 2026-09

90/100 stars

Buy from Supplier

m14 human melanoma cells (ATCC)

ATCC m14 human melanoma cells

MTT assay performed after 72 h of incubation with the formulations under study. B16 cells: upper left panel; D4M cells: upper right panel; <t>M14</t> cells: lower left panel; A2058 cells: lower right panel. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

M14 Human Melanoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m14+cells/M1%2E4/pmc09740980-73-2-15
Average 95 stars, based on 1 article reviews

m14 human melanoma cells - by Bioz Stars, 2026-09

95/100 stars

Buy from Supplier

Image Search Results

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Identification of differentially expressed genes based on ImmuneScore and StromalScore. ( A-C ) Overall survival analysis of high and low Immune/Stromal/ESTIMATE Scores in patients with metastatic melanoma using the ESTIMATE algorithm. ( D , E ) Heatmap depicting the top 100 DEmRNAs with the most significant p-values between high and low Immune/Stromal Scores (|log Fold change|>1.9, p < 0.001). Blue represents downregulation, while red represents upregulation. ( F ) Venn diagram showing the upregulated and downregulated genes commonly intersecting between Stromal and Immune Scores, with green indicating immune-related DEmRNAs and purple indicating stromal-related DEmRNAs. ( G ) Construction of a protein-protein interaction network for overlapping genes using the STRING online tool (confidence score > 0.900) and Cytoscape software. ( H ) The top 15 genes ranked by nodes in the PPI network are shown in the graph. ( I ) Univariate Cox analysis was conducted on genes with a node count of 15 or more in the PPI network to identify differentially expressed genes significantly associated with prognosis

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Software

The expression of PTPN6 and its correlation analysis with survival. ( A ) Boxplot showing differential expression of PTPN6 in metastatic melanoma samples and normal skin tissue samples. ( B ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with overall survival. ( C ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with progression-free survival. ( D ) RT-qPCR assay analyzed the transcription level of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( E ) Western blotting assay analyzed the expression of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. (** p < 0.01, *** p < 0.001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: The expression of PTPN6 and its correlation analysis with survival. ( A ) Boxplot showing differential expression of PTPN6 in metastatic melanoma samples and normal skin tissue samples. ( B ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with overall survival. ( C ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with progression-free survival. ( D ) RT-qPCR assay analyzed the transcription level of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( E ) Western blotting assay analyzed the expression of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. (** p < 0.01, *** p < 0.001)

Techniques: Expressing, Quantitative Proteomics, Quantitative RT-PCR, Western Blot

Overexpression of PTPN6 inhibited the proliferation and viability of metastatic melanoma cells in vitro. ( A–C ) RT-qPCR and Western blotting assays were performed to evaluate the mRNA and protein expression of PTPN6 in A2058 and M14 cells after transfection with PTPN6. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( D , E ) CCK8 assay was used to determine the impact of PTPN6 overexpression on the proliferation of A2058 and M14 cells. ( F , G ) EdU assay was conducted to assess the impact of PTPN6 overexpression on the viability of A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as the mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 inhibited the proliferation and viability of metastatic melanoma cells in vitro. ( A–C ) RT-qPCR and Western blotting assays were performed to evaluate the mRNA and protein expression of PTPN6 in A2058 and M14 cells after transfection with PTPN6. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( D , E ) CCK8 assay was used to determine the impact of PTPN6 overexpression on the proliferation of A2058 and M14 cells. ( F , G ) EdU assay was conducted to assess the impact of PTPN6 overexpression on the viability of A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as the mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques: Over Expression, In Vitro, Quantitative RT-PCR, Western Blot, Expressing, Transfection, CCK-8 Assay, EdU Assay, Standard Deviation

Overexpression of PTPN6 suppressed the migration and invasion of metastatic melanoma cells in vitro. ( A , B ) Wound healing assay was performed to determine the impact of PTPN6 overexpression on the migration of A2058 and M14 cells. ( C , D ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 overexpression on the migration and invasion of A2058 and M14 cells. ( E , F ) Western blotting assay was conducted to verify the expression of migration and invasion-related genes, including MMP2, MMP9, E-cadherin, and Vimentin, in control, NC, and PTPN6 overexpression groups in A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 suppressed the migration and invasion of metastatic melanoma cells in vitro. ( A , B ) Wound healing assay was performed to determine the impact of PTPN6 overexpression on the migration of A2058 and M14 cells. ( C , D ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 overexpression on the migration and invasion of A2058 and M14 cells. ( E , F ) Western blotting assay was conducted to verify the expression of migration and invasion-related genes, including MMP2, MMP9, E-cadherin, and Vimentin, in control, NC, and PTPN6 overexpression groups in A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques: Over Expression, Migration, In Vitro, Wound Healing Assay, Western Blot, Expressing, Control, Standard Deviation

Overexpression of PTPN6 inhibited the activity of the JAK2-STAT3 signaling pathway in metastatic melanoma cells. ( A-D ) Western blotting assay was used to assess the expression of p-JAK2, JAK2, p-STAT3, and STAT3 after transfection with the PTPN6 overexpression plasmid. (**** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 inhibited the activity of the JAK2-STAT3 signaling pathway in metastatic melanoma cells. ( A-D ) Western blotting assay was used to assess the expression of p-JAK2, JAK2, p-STAT3, and STAT3 after transfection with the PTPN6 overexpression plasmid. (**** p < 0.0001)

Techniques: Over Expression, Activity Assay, Western Blot, Expressing, Transfection, Plasmid Preparation

PTPN6 may affect immune cell infiltration and immune therapy response in metastatic melanoma. ( A ) TIMER 2.0 online analysis tool was used to examine the relationship between PTPN6 and the levels of infiltration of 14 immune cells and tumor purity in metastatic melanoma. ( B ) The correlation between PTPN6 and immune checkpoint therapy (alone or in combination with PD-1 or CTLA-4) response was analyzed using the TCIA database. ( C , D ) Western blotting assay was used to detect PD-L1 expression after PTPN6 overexpression (* p < 0.05, ** p < 0.01)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: PTPN6 may affect immune cell infiltration and immune therapy response in metastatic melanoma. ( A ) TIMER 2.0 online analysis tool was used to examine the relationship between PTPN6 and the levels of infiltration of 14 immune cells and tumor purity in metastatic melanoma. ( B ) The correlation between PTPN6 and immune checkpoint therapy (alone or in combination with PD-1 or CTLA-4) response was analyzed using the TCIA database. ( C , D ) Western blotting assay was used to detect PD-L1 expression after PTPN6 overexpression (* p < 0.05, ** p < 0.01)

Techniques: Western Blot, Expressing, Over Expression

Knockdown of PTPN6 promoted the proliferation, migration, and invasion of metastatic melanoma cells in vitro. ( A , B ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cells after transfection with GV341-PTPN6-3FLAG-SV40-puromycin lentivirus. ( C , D ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cell lines stably overexpressing PTPN6 after transiently transfected siPTPN6 or siNC. ( E , F ) CCK8 assay was used to determine the impact of PTPN6 knockdown on the proliferation of cells. ( G , H ) Wound healing assay was performed to determine the impact of PTPN6 knockdown on the migration of cells. ( I , J ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 knockdown on the migration and invasion of cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Knockdown of PTPN6 promoted the proliferation, migration, and invasion of metastatic melanoma cells in vitro. ( A , B ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cells after transfection with GV341-PTPN6-3FLAG-SV40-puromycin lentivirus. ( C , D ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cell lines stably overexpressing PTPN6 after transiently transfected siPTPN6 or siNC. ( E , F ) CCK8 assay was used to determine the impact of PTPN6 knockdown on the proliferation of cells. ( G , H ) Wound healing assay was performed to determine the impact of PTPN6 knockdown on the migration of cells. ( I , J ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 knockdown on the migration and invasion of cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques: Knockdown, Migration, In Vitro, Western Blot, Expressing, Transfection, Stable Transfection, CCK-8 Assay, Wound Healing Assay, Standard Deviation

MTT assay performed after 72 h of incubation with the formulations under study. B16 cells: upper left panel; D4M cells: upper right panel; M14 cells: lower left panel; A2058 cells: lower right panel. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

Journal: Nanomaterials

Article Title: Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment

doi: 10.3390/nano12234233

Figure Lengend Snippet: MTT assay performed after 72 h of incubation with the formulations under study. B16 cells: upper left panel; D4M cells: upper right panel; M14 cells: lower left panel; A2058 cells: lower right panel. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

Article Snippet: A2058 and M14 human melanoma cells and B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: MTT Assay, Incubation

Clonogenic assay with B16 (upper left panel), D4M (upper right panel), M14 (lower left panel), and A2058 (lower right panel) melanoma cells. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Cells were treated with the formulations under study for 3 h. Afterwards, the medium was changed, and cells were cultured in drug-free medium for an additional 7 days. Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

Journal: Nanomaterials

Article Title: Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment

doi: 10.3390/nano12234233

Figure Lengend Snippet: Clonogenic assay with B16 (upper left panel), D4M (upper right panel), M14 (lower left panel), and A2058 (lower right panel) melanoma cells. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Cells were treated with the formulations under study for 3 h. Afterwards, the medium was changed, and cells were cultured in drug-free medium for an additional 7 days. Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

Article Snippet: A2058 and M14 human melanoma cells and B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Clonogenic Assay, Cell Culture

Journal: Nanomaterials

Article Title: Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment

doi: 10.3390/nano12234233

Figure Lengend Snippet: Hypothesized sorafenib (SOR) mechanism of action: ( a ) SOR release profile in culture medium, and ( b ) SOR internalization migration assay (B16, M14 melanoma cells): *** p < 0.005, * p < 0.05, # p < 0.01. ( c ) Hypothesized cellular pathways. Abbreviations: FCS: fetal calf serum; IL: Intralipid ® 10%; RAF: rapidly accelerated fibrosarcoma kinases; RTK: receptor tyrosine kinases.

Article Snippet: A2058 and M14 human melanoma cells and B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration

Review

Structured Review

Images

Related Articles

Cell Culture:

other:

Expressing:

Similar Products

Image Search Results