methyl β cyclodextrin mβcd  (TargetMol)

Journal: Nature Communications

Article Title: Recycling senescent cell lipids for targeted senotherapy

doi: 10.1038/s41467-026-70486-0

Figure Lengend Snippet: a Schematic comparing targeted senorecycle therapy vs. senolytics: targeting, non-apoptotic lipid extraction, sustained efficacy, dual chondroprotection-lubrication. b Experimental workflow for comparative analysis: Following mild senescence (IL-1β, 5 ng/mL,8 h, Bleomycin, 5 μg/mL, 8 h) induction, cells were treated with either D + Q (Dasatinib, 200 nM plus Quercetin, 10 μm) or MβCD@ICAM1-NPs for 24 h (MβCD 50 μg/mL), followed by parallel qPCR analysis of related genes and ELISA quantification of secreted proteins. c ELISA results of inflammatory mediators (IL-6, Mmp9, Mmp13, Ccl2) and DAMPs (Hmgb1) in conditioned media from senolytic- or NP-treated senescence (IL-1β) group. n = 8 biologically independent cell cultures (mean ± s.d.). d Workflow for detecting the effects of the two therapies on normal proliferating primary chondrocytes for 4 days: qPCR analysis of collected cells and ELISA quantification of secreted proteins in supernatants. e Trends of secretion levels of IL-6, Mmp9, Mmp13 and Ccl2 in the supernatant across two therapies ( n = 4, mean ± s.d.). f Trends of qPCR results of IL-6 , Mmp13 , Col2a1 and Aggrecan in cells across two treatments ( n = 3, mean ± s.d.). g Flow chart of the friction coefficient detection under different treatments. Fresh control or OA implants from the same part were collected, and the overall friction coefficient was detected after incubated with senolytics (D + Q) or MINH for 24 h. h COF-time curves (1200 s) across treatment groups. i Schematic diagram of in vivo therapeutic comparison using p16-3MR mice. j Quantitative analysis of 50% paw withdraw threshold across groups. n = 8 mice per group (mean ± s.d.). k Visualization results of the medial subchondral bone plate in each group(top) and the 3D reconstruction of murine knee joints (bottom). The heatmap shows its bone density distribution. Yellow arrows indicate osteophyte formation. l Representative images of Safranin-O staining results of knee joint sections across groups. The same position (black dashed box) in the above figure is enlarged and shown below (scale bar, 50 μm). m Representative images of immunohistochemical staining (Aggrecan, Col2a1, IL-6 and Mmp13) of medial tibia sections of knee joints across groups (scale bar, 50 μm). P values were determined using one-way ANOVA with a Tukey post-hoc test ( c , j ). Source data are provided as a Source Data file.

Article Snippet: MβCD (MCE, HY-101461, 50 μg/mL), Dasatinib (MCE, HY-10181, 200 nM), Quercetin (MCE, HY-18085, 10 μM).

Techniques: Extraction, Enzyme-linked Immunosorbent Assay, Control, Incubation, In Vivo, Comparison, Staining, Immunohistochemical staining

Journal: Journal of Virology

Article Title: Bovine coronavirus enters HRT-18 cells via membrane fusion and clathrin-mediated endocytosis in a low pH-, dynamin-, cholesterol-, microtubule-, Rab7-, and Rab11-dependent manner

doi: 10.1128/jvi.01274-25

Figure Lengend Snippet: BCoV requires cholesterol to enter HRT-18 cells. ( A ) The maximum safe concentrations of MβCD were determined using the CCK-8 assay. ( B ) Western blot analysis was used to evaluate the BCoV N protein expression levels, with grayscale analysis performed and presented as a bar graph. ( C ) RT-qPCR was performed to assess the BCoV gene copy numbers. ( D ) TCID 50 assay was used to measure the BCoV viral titers in the cell supernatant. ( E ) IFA was used to detect the number of BCoV-infected cells. Scale bar = 100 µm. ( F ) RT-qPCR was used to evaluate the effect of MβCD on the viral entry. ( G ) RT-qPCR was used to evaluate the effect of MβCD on the viral attachment. Data are presented as the mean ± SD of three independent experiments (not significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The inhibitors used in this study included SSAA09E3 (Cat HY-138102, MedChemExpress), a novel inhibitor that blocks the fusion of the viral membrane with the host cell membrane; CPZ (Cat C0982, Sigma), a clathrin-mediated endocytosis inhibitor; nystatin (Cat 475914, Sigma), a caveolae inhibitor that acts as a sterol-binding agent disrupting caveolae; blebbistatin (Cat 203391, Sigma), an inhibitor of micropinocytosis; dynasore (Cat T1848, TargetMol), a dynamin inhibitor; MβCD (Cat T4072, TargetMol), a cholesterol depletion inhibitor; chloroquine (Cat S6999, Selleck) and NH 4 Cl (Cat A9434, Sigma), a potent inhibitor of V-ATPase and a specific inhibitor of acidification of endosomal vesicles; colchicine (Cat HY-16569, MedChemExpress), which inhibits the polymerization of tubulin; E64d (Cat S7393, Selleck), a cathepsin inhibitor; and camostat (Cat HY-13512, MedChemExpress), a TMPRSS2 inhibitor.

Techniques: CCK-8 Assay, Western Blot, Expressing, Quantitative RT-PCR, Infection