m hominis atcc 23114  (ATCC)

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: COG classification of genes. (A) Clusters of orthologous genes (COGs) distribution among the 52 M. hominis strains associated with infertility. (B) COGs distribution among the 10 M. hominis strains associated with genital infections. Bars in green, red, and blue, represent the core genome, the accessory genes (shell genes), and unique genes (cloud genes), respectively.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: Single nucleotide polymorphism (SNP) distribution among M. hominis strains associated with gynecological infections (red boxplot) and infertility (blue boxplot). Student's t -test was used to assess for statistical significant difference ( P < 0.05) between the mean SNPs count values of strains linked to infertility and gynecological infections. * Represents the mean SNP count of each boxplot.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: TreeWAS-based phylogenetic tree illustrating the relatedness between the 62 Tunisian M. hominis clinical strains based on pathotype association. Strains in red are associated with gynecological infections pathotype and strains in blue are associated with infertility pathotype.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Microorganisms

Article Title: Mycoplasma hominis Causes DNA Damage and Cell Death in Primary Human Keratinocytes

doi: 10.3390/microorganisms10101962

Figure Lengend Snippet: Mycoplasma hominis infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.

Article Snippet: The strain M. hominis (ATCC 23114-PG-21) was obtained from the ICB/USP Mycoplasma laboratory and grown in 100 mL of SP4 medium [ ] until the mid-log phase.

Techniques: Infection, Control, One-tailed Test, MANN-WHITNEY, Clonogenic Assay, Staining