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human t lymphoblast cell line  (ATCC)


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    ATCC human t lymphoblast cell line
    Human T Lymphoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lymphoblastoid+t/13C1+Human+Lymphoblastoid+cell+Line/pm41362094-413-7-16
    Average 94 stars, based on 80 article reviews
    human t lymphoblast cell line - by Bioz Stars, 2026-11
    94/100 stars

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    Expressing:

    Article Title: TCR activation stimulates regulated intramembrane proteolysis of L-selectin by presenilin 1 and localized proteasomal degradation of the cytoplasmic tail.
    Article Snippet: .. L-selectin expressing T cells Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552) were sequentially transduced to co-express an HIV-1 Gag SLYNTVATL-specific TCR (868) with either human L- V5/His, non-tagged L-selectin or L-selectin-GFP (25) using methods described previously (11). .. L-selectin expressing T cells Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552) were sequentially transduced to co-express an HIV-1 Gag SLYNTVATL-specific TCR (868) with either human L- V5/His, non-tagged L-selectin or L-selectin-GFP (25) using methods described previously (11).

    Transduction:

    Article Title: ADAM17-dependent proteolysis of L-selectin promotes early clonal expansion of cytotoxic T cells
    Article Snippet: .. Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552), were sequentially transduced with lentiviral particles isolated from transiently transfected 293 T cells to express an HIV-1 Gag SLYNTVATL-specific TCR (868) and either wildtype L-selectin or shedding-resistant ∆M-N human L-selectin . .. Full-length and ∆M-N-L-selectin were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent), and the PCR product was cleaved with BamHI and XhoI before ligation into BamHI-linearized pSxW using In-fusion (Clontech).

    Isolation:

    Article Title: ADAM17-dependent proteolysis of L-selectin promotes early clonal expansion of cytotoxic T cells
    Article Snippet: .. Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552), were sequentially transduced with lentiviral particles isolated from transiently transfected 293 T cells to express an HIV-1 Gag SLYNTVATL-specific TCR (868) and either wildtype L-selectin or shedding-resistant ∆M-N human L-selectin . .. Full-length and ∆M-N-L-selectin were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent), and the PCR product was cleaved with BamHI and XhoI before ligation into BamHI-linearized pSxW using In-fusion (Clontech).

    Transfection:

    Article Title: ADAM17-dependent proteolysis of L-selectin promotes early clonal expansion of cytotoxic T cells
    Article Snippet: .. Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552), were sequentially transduced with lentiviral particles isolated from transiently transfected 293 T cells to express an HIV-1 Gag SLYNTVATL-specific TCR (868) and either wildtype L-selectin or shedding-resistant ∆M-N human L-selectin . .. Full-length and ∆M-N-L-selectin were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent), and the PCR product was cleaved with BamHI and XhoI before ligation into BamHI-linearized pSxW using In-fusion (Clontech).



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    Image Search Results


    Growth inhibitory activities of target compounds against CCRF-HSB-2 and KB tumour cell lines.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Novel alloxazine analogues: design, synthesis, and antitumour efficacy enhanced by kinase screening, molecular docking, and ADME studies

    doi: 10.1080/14756366.2024.2398551

    Figure Lengend Snippet: Growth inhibitory activities of target compounds against CCRF-HSB-2 and KB tumour cell lines.

    Article Snippet: Two human tumour cell lines, CCRF-HSB-2 (human T-cell acute lymphoblastoid leukaemia) and KB (human oral epidermoid carcinoma), were obtained from the National Institute of Biomedical Innovation, Health, and Nutrition (NIBIOHN)-JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan) with the reference numbers: JCRB0031 and JCRB9027, respectively.

    Techniques: Activity Assay