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ly6g gr1  (Bio-Rad)


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    Structured Review

    Bio-Rad ly6g gr1
    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for <t>Ly6G</t> (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.
    Ly6g Gr1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ly6g+gr1/Rat+anti+Mouse+Gr-1/bio_rxiv__64898__2026__04__21__719989-90-30-32
    Average 93 stars, based on 149 article reviews
    ly6g gr1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury"

    Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

    Journal: bioRxiv

    doi: 10.64898/2026.04.21.719989

    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.
    Figure Legend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Techniques Used: Immunofluorescence, Staining, Labeling

    Related Articles

    Western Blot:

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. Antibodies The following antibodies were used for Western blotting and immunohistochemistry staining: β-actin (Sigma, A-1978), α-tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF-κB p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blots and immunohistochemistry staining: β-actin (Sigma, A-1978), α-Tubulin (Sigma, T6074), Histone H3 (Cell signaling, 14269), NF-κB p65 (Cell signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, by guest on D ecem ber 19, 2019 http://w w w .jbc.org/ D ow nloaded from MCA1439T), F4/80 (Bio-Rad, MCA497R), Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blotting and immunohistochemistry staining: -actin (Sigma, A-1978), -tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF- B p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (BioRad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad,MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blotting and immunohistochemistry staining: β-actin (Sigma, A-1978), α-tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF-κB p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Immunohistochemistry:

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. Antibodies The following antibodies were used for Western blotting and immunohistochemistry staining: β-actin (Sigma, A-1978), α-tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF-κB p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blots and immunohistochemistry staining: β-actin (Sigma, A-1978), α-Tubulin (Sigma, T6074), Histone H3 (Cell signaling, 14269), NF-κB p65 (Cell signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, by guest on D ecem ber 19, 2019 http://w w w .jbc.org/ D ow nloaded from MCA1439T), F4/80 (Bio-Rad, MCA497R), Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blotting and immunohistochemistry staining: -actin (Sigma, A-1978), -tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF- B p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (BioRad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad,MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blotting and immunohistochemistry staining: β-actin (Sigma, A-1978), α-tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF-κB p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Staining:

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. Antibodies The following antibodies were used for Western blotting and immunohistochemistry staining: β-actin (Sigma, A-1978), α-tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF-κB p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blots and immunohistochemistry staining: β-actin (Sigma, A-1978), α-Tubulin (Sigma, T6074), Histone H3 (Cell signaling, 14269), NF-κB p65 (Cell signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, by guest on D ecem ber 19, 2019 http://w w w .jbc.org/ D ow nloaded from MCA1439T), F4/80 (Bio-Rad, MCA497R), Ly6G/Gr1 (Bio-Rad, MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blotting and immunohistochemistry staining: -actin (Sigma, A-1978), -tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF- B p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (BioRad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad,MCA2387T). ..

    Article Title: Disruption of hepatic small heterodimer partner induces dissociation of steatosis and inflammation in experimental nonalcoholic steatohepatitis
    Article Snippet: .. The following antibodies were used for Western blotting and immunohistochemistry staining: β-actin (Sigma, A-1978), α-tubulin (Sigma, T6074), histone H3 (Cell Signaling, 14269), NF-κB p65 (Cell Signaling, 8242), CD3 (Bio-Rad, MCA500GT), CD4 (Bio-Rad, MCA2691T), CD8 (Bio-Rad, MCA2694T), CD19 (Bio-Rad, MCA1439T), F4/80 (Bio-Rad, MCA497R), and Ly6G/Gr1 (Bio-Rad, MCA2387T). ..



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    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for <t>Ly6G</t> (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.
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    Image Search Results


    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Journal: bioRxiv

    Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

    doi: 10.64898/2026.04.21.719989

    Figure Lengend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

    Techniques: Immunofluorescence, Staining, Labeling