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luclite kit  (Packard Instruments)

 
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    Packard Instruments luclite kit
    Luclite Kit, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luclite+system/luclite+kit/pm29356860-54-7-9
    Average 90 stars, based on 1 article reviews
    luclite kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: Alteration of the glucocorticoid receptor subcellular localization by non steroidal compounds.
    Article Snippet: The glucocorticoid receptor (GR) engages transient or stable interactions with chaperones (hsp90, hsp70), co-chaperones (p60/ hop, hsp40) and several other polypeptides such as immunophilins (Cyp40, FKBP59) and p23 to achieve a high a nity ligand binding state.. This complex dissociates in response to hormonal stimuli and holo-GR translocates into the nucleus, where it regulates the activity of glucocorticoid-sensitive genes.. GR activity is controlled through its ligand binding domain by steroids displaying either agonistic or antagonistic activity.

    Article Title: Allosteric Regulation of the Discriminative Responsiveness of Retinoic Acid Receptor to Natural and Synthetic Ligands by Retinoid X Receptor and DNA
    Article Snippet: .. The luciferase assay was performed with the LucLite system (Packard Instruments, Rungis, France) according to the manufacturer’s guidelines, and activity (as relative luciferase units was measured with a LumiCount plate reader (Packard). ..

    Article Title: Critical role of the H6-H7 loop in the conformational adaptation of all-trans retinoic acid and synthetic retinoids within the ligand-binding site of RARalpha.
    Article Snippet: .. Cells were treated with the different retinoids at a final concentration of 10 6 M for 16 h. The luciferase assay was performed with the LucLite system (Packard Instruments, Rungis, France) according to the manufacturer’s instructions and activity (as relative units) was measured with a Lumi-Count reader. .. In vitro transcription, translation and limited proteolytic digestion Wild-type and receptor mutants in pSG5 were transcribed and in vitro translated in the presence of [35S]methionine by using rabbit reticulocyte lysates (Quick T7 TnT kit, Promega).

    Activity Assay:

    Article Title: Allosteric Regulation of the Discriminative Responsiveness of Retinoic Acid Receptor to Natural and Synthetic Ligands by Retinoid X Receptor and DNA
    Article Snippet: .. The luciferase assay was performed with the LucLite system (Packard Instruments, Rungis, France) according to the manufacturer’s guidelines, and activity (as relative luciferase units was measured with a LumiCount plate reader (Packard). ..

    Article Title: Critical role of the H6-H7 loop in the conformational adaptation of all-trans retinoic acid and synthetic retinoids within the ligand-binding site of RARalpha.
    Article Snippet: .. Cells were treated with the different retinoids at a final concentration of 10 6 M for 16 h. The luciferase assay was performed with the LucLite system (Packard Instruments, Rungis, France) according to the manufacturer’s instructions and activity (as relative units) was measured with a Lumi-Count reader. .. In vitro transcription, translation and limited proteolytic digestion Wild-type and receptor mutants in pSG5 were transcribed and in vitro translated in the presence of [35S]methionine by using rabbit reticulocyte lysates (Quick T7 TnT kit, Promega).

    Concentration Assay:

    Article Title: Critical role of the H6-H7 loop in the conformational adaptation of all-trans retinoic acid and synthetic retinoids within the ligand-binding site of RARalpha.
    Article Snippet: .. Cells were treated with the different retinoids at a final concentration of 10 6 M for 16 h. The luciferase assay was performed with the LucLite system (Packard Instruments, Rungis, France) according to the manufacturer’s instructions and activity (as relative units) was measured with a Lumi-Count reader. .. In vitro transcription, translation and limited proteolytic digestion Wild-type and receptor mutants in pSG5 were transcribed and in vitro translated in the presence of [35S]methionine by using rabbit reticulocyte lysates (Quick T7 TnT kit, Promega).



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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
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    Image Search Results


    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation

    doi: 10.1016/j.mcpro.2022.100488

    Figure Lengend Snippet: Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

    Article Snippet: Cells were cultured 48 h post-transfection, and luciferase activity was analyzed using Luclite plus Reporter Gene Assay (PerkinElmer) measured by Glo-Max Microplate Multimode reader (Promega).

    Techniques: Phospho-proteomics, Expressing, Transfection, Luciferase, Plasmid Preparation, Activity Assay, Reporter Gene Assay, Activation Assay, SDS Page, Control, Virus