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ltl fitc  (Vector Laboratories)


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    Structured Review

    Vector Laboratories ltl fitc
    Ltl Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ltl+fitc/Biotinylated+Lotus+Tetragonolobus+Lectin+(LTL)/pm41898549-239-31-32
    Average 96 stars, based on 409 article reviews
    ltl fitc - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids
    Article Snippet: .. Kidney organoids on day 26 of differentiation cultured at the air-liquid interface in transwells were added to medium with pHrodoTM Red Dextran, 10,000 MW, for Endocytosis (Invitrogen, P10361) at 10 mg/mL and incubated at 37 °C in a 5% CO 2 incubator for 24 h. Organoids were then washed 3 times with ice-cold DPBS (−) and loaded into DPBS (−) containing LTL-FITC ((1:100) Vector Laboratories, FL-1321) for 2 h at 4 °C. .. Stained kidney organoids were observed for fluorescence using a CV8000 (Yokogawa).

    Incubation:

    Article Title: Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids
    Article Snippet: .. Kidney organoids on day 26 of differentiation cultured at the air-liquid interface in transwells were added to medium with pHrodoTM Red Dextran, 10,000 MW, for Endocytosis (Invitrogen, P10361) at 10 mg/mL and incubated at 37 °C in a 5% CO 2 incubator for 24 h. Organoids were then washed 3 times with ice-cold DPBS (−) and loaded into DPBS (−) containing LTL-FITC ((1:100) Vector Laboratories, FL-1321) for 2 h at 4 °C. .. Stained kidney organoids were observed for fluorescence using a CV8000 (Yokogawa).

    Article Title: Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids
    Article Snippet: .. LTL-FITC (FL-1321, Vector Laboratories) was added at a concentration of 1:100, and the mixture was incubated for 30 min at 4 °C with a rotator. ..

    Article Title: Nephron progenitor fate is modulated by angiotensin type 1 receptor signaling in human kidney organoids.
    Article Snippet: Nephron progenitor fate is modulated by angiotensin type 1 receptor signaling in human kidney organoids Hyunjae Chung, Waleed Rahmani, Sarthak Sinha, Aysa Imanzadeh, Alexander Pun, Rohit Arora, Arzina Jaffer, Jeff Biernaskie, Justin Chun D ow nloaded from https://academ ic.oup.com /stm cls/advance-article/doi/10.1093/stm cls/sxaf012/8087999 by guest on 21 M arch 2025 Ac ce pte d M an us cri pt © The Author(s) 2025.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons AttributionNonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits noncommercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Nephron progenitor fate is modulated by angiotensin type 1 receptor signaling in human kidney organoids
    Article Snippet: Primary antibodies to NPHS1 (R&D Systems, Cat. #AF4269), PDGFR-β (Cell Signaling, Cat. #4564), E-Cadherin (BD Transduction laboratories, Cat. #610181), Synaptopodin (SCBT, Cat. #sc-515842), SIX2 (ProteinTech, Cat. #11562-1-AP), CD31 (BD Pharmingen, 555444), and AT1R (ThermoFisher, Cat. #MA5-42535). .. After 6 washes with wash buffer (0.3% Triton X-100 in PBS) for 10 minutes each wash, organoids were incubated with fluorescent conjugated secondary antibodies (Alexa Fluor donkey anti-mouse 405 (ThermoFisher), donkey anti-rabbit 568 (Invitrogen), donkey anti-sheep 647 (Invitrogen), donkey anti-mouse 647 (Invitrogen) or LTL-FITC (Vector laboratories, Cat. #FL-1321-2) for 2 hours at room temperature. ..

    Blocking Assay:

    Article Title: Dynamic Glycoprotein Hyposialylation Promotes Chemotherapy Evasion and Metastatic Seeding of Quiescent Circulating Tumor Cell Clusters in Breast Cancer
    Article Snippet: .. Cells were blocked with carbofree blocking solution (Vector Laboratories, SP-5040) and stained with antibodies for proteins (CD45, epithelial marker EpCAM, and other blood cell lineage markers) and lectins for glycans, such as SNA-FITC, MALII-FITC, PHA-L-PE, LTL-FITC, RCA-Rho, LEL-APC, ConA-PE (Vector Laboratories), and MALII-FITC (GlycoMATRIX). ..

    Staining:

    Article Title: Dynamic Glycoprotein Hyposialylation Promotes Chemotherapy Evasion and Metastatic Seeding of Quiescent Circulating Tumor Cell Clusters in Breast Cancer
    Article Snippet: .. Cells were blocked with carbofree blocking solution (Vector Laboratories, SP-5040) and stained with antibodies for proteins (CD45, epithelial marker EpCAM, and other blood cell lineage markers) and lectins for glycans, such as SNA-FITC, MALII-FITC, PHA-L-PE, LTL-FITC, RCA-Rho, LEL-APC, ConA-PE (Vector Laboratories), and MALII-FITC (GlycoMATRIX). ..

    Marker:

    Article Title: Dynamic Glycoprotein Hyposialylation Promotes Chemotherapy Evasion and Metastatic Seeding of Quiescent Circulating Tumor Cell Clusters in Breast Cancer
    Article Snippet: .. Cells were blocked with carbofree blocking solution (Vector Laboratories, SP-5040) and stained with antibodies for proteins (CD45, epithelial marker EpCAM, and other blood cell lineage markers) and lectins for glycans, such as SNA-FITC, MALII-FITC, PHA-L-PE, LTL-FITC, RCA-Rho, LEL-APC, ConA-PE (Vector Laboratories), and MALII-FITC (GlycoMATRIX). ..

    Concentration Assay:

    Article Title: Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids
    Article Snippet: .. LTL-FITC (FL-1321, Vector Laboratories) was added at a concentration of 1:100, and the mixture was incubated for 30 min at 4 °C with a rotator. ..

    other:




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    Vector Laboratories fitc conjugated ltl
    (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of <t>FITC-sinistrin.</t> Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.
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    (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of <t>FITC-sinistrin.</t> Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.
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    Image Search Results


    (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of FITC-sinistrin. Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.

    Journal: bioRxiv

    Article Title: Life in the fast lane: Functional consequences of male-female dynamic differences in the renal autoregulation of flow

    doi: 10.1101/2025.09.12.675896

    Figure Lengend Snippet: (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of FITC-sinistrin. Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.

    Article Snippet: The following antibodies were employed in this study recognizing KIM-1 (kidney injury molecule-1, Rat, 1:500, R&D Systems, MAB1817), FITC-conjugated LTL (Lotis Tetragonolobus Lectin, 1:1000, Vector Laboratories, FL-1321).

    Techniques: Microscopy, Clinical Proteomics, Fluorescence, Magnetic Resonance Imaging, Filtration, Sampling