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loading control hrp conjugated β actin antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology loading control hrp conjugated β actin antibody
    Loading Control Hrp Conjugated β Actin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 46264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/loading+control/%CE%B2-Actin+Antibody/pmc13023379-203-15-23
    Average 96 stars, based on 46264 article reviews
    loading control hrp conjugated β actin antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Control:

    Article Title: Development of a Conditional Replication System Using a Lassa Virus Glycoprotein Complex-Encoding Retroviral Vector for Isolating Resistant Variants to Inhibitors in BSL-2.
    Article Snippet: To detect the LASV GPC, the membrane was incubated with a 1/500- diluted mouse anti-LASV GP1 monoclonal antibody EBS-I-301 (PROGEN, Heidelberg, Germany) followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody (Bio-Rad). .. For detection of actin protein as a loading control, the membrane was incubated with a 1/2000-diluted mouse anti-actin antibody sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody. .. Finally, antibody-bound proteins were visualized using an HRP substrate (Bio-Rad) by C-DiGit scanner (LI-COR, Lincoln, NB, USA).

    Article Title: A shift in the cellular redox state redirects aspartate for export under glucose deprivation.
    Article Snippet: After blocking in 5% bovine serum albumin (BSA)-TBST or milk-TBST for 1 hour at room temperature, membranes were probed with GOT1 antibody (ab239487, Abcam, Cambridge, UK; milk) 1:1000, GOT2 antibody (HPA018139, Sigma-Aldrich; milk) 1:2000, or VDAC1/2/3 mitochondrial loading control antibody (ab15895, Abcam; BSA) 1:5000. .. As a loading control, membranes were incubated with a β-actin antibody (sc-47778, clone C4, Santa Cruz Biotechnology, Dallas, TX) 1:3000 in 5% milk-TBST or with GAPDH-antibody conjugated to peroxidase (sc-365062) 1:5000 in 5% BSA-TBST. ..

    Article Title: N -( p -Coumaroyl) Serotonin Ameliorates LPS-Induced Inflammation in BV2 Microglia via MAPK/NF-κB Inactivation and HO-1/NQO1 Upregulation.
    Article Snippet: Immunoreactive bands were visualized using an enhanced chemiluminescence (ECL) system (Thermo Fisher Scientific, Inc.). .. Densitometric quantification was subsequently carried out using ImageJ software (version 1.52a; National Institutes of Health, Bethesda, MD, USA), with β-actin serving as the internal loading control. https://doi.org/10.3390/cimb48020232 β-actin Santa Cruz Biotech (Dallas, TX, USA) #sc-47778 43 M Antibodies were used at 1:1000 dilution (1:5000 for β-actin). p-, phosphorylated; MW, molecular weight; R, rabbit; M, mouse. ..

    Article Title: A shift in the cellular redox state redirects aspartate for export under glucose deprivation
    Article Snippet: After blocking in 5% bovine serum albumin (BSA)-TBST or milk-TBST for 1 hour at room temperature, membranes were probed with GOT1 antibody (ab239487, Abcam, Cambridge, UK; milk) 1:1000, GOT2 antibody (HPA018139, Sigma-Aldrich; milk) 1:2000, or VDAC1/2/3 mitochondrial loading control antibody (ab15895, Abcam; BSA) 1:5000. .. As a loading control, membranes were incubated with a β-actin antibody (sc-47778, clone C4, Santa Cruz Biotechnology, Dallas, TX) 1:3000 in 5% milk-TBST or with GAPDH-antibody conjugated to peroxidase (sc-365062) 1:5000 in 5% BSA-TBST. ..

    Article Title: Development of a Conditional Replication System Using a Lassa Virus Glycoprotein Complex-Encoding Retroviral Vector for Isolating Resistant Variants to Inhibitors in BSL-2
    Article Snippet: To detect the LASV GPC, the membrane was incubated with a 1/500-diluted mouse anti-LASV GP1 monoclonal antibody EBS-I-301 (PROGEN, Heidelberg, Germany) followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody (Bio-Rad). .. For detection of actin protein as a loading control, the membrane was incubated with a 1/2000-diluted mouse anti-actin antibody sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody. .. Finally, antibody-bound proteins were visualized using an HRP substrate (Bio-Rad) by C-DiGit scanner (LI-COR, Lincoln, NB, USA).

    Article Title: A novel spliceosomopathy caused by de novo SF3B3 variants
    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against FLAG (F3165, Sigma-Aldrich) and GFP (11814460001, Roche). .. GAPDH was used as a loading control, detected with mouse monoclonal anti-GAPDH (sc-47724, Santa Cruz Biotechnology Inc). ..

    Article Title: Upregulation of a CFTR mRNA isoform has therapeutic potential for the treatment of 3′ CFTR PTC variants
    Article Snippet: .. Western blot analyses were performed as described previously, except the loading control, ACTB was detected with beta actin antibody C4 (sc-47778) from Santa Cruz Biotechnology. .. Blots were imaged using the ChemiDoc MP Imaging System (Bio-Rad) or ChemiDoc XRS+ Gel Documentation System (Bio-Rad) and analyzed with the accompanying image analysis software.

    Article Title: A novel spliceosomopathy caused by de novo SF3B3 variants.
    Article Snippet: Membranes were incubated overnight at 4°C with primary antibodies against FLAG (F3165, Sigma-Aldrich) and GFP (11814460001, Roche). .. GAPDH was used as a loading control, detected with mouse monoclonal anti-GAPDH (sc-47724, Santa Cruz Biotechnology Inc). ..

    Membrane:

    Article Title: Development of a Conditional Replication System Using a Lassa Virus Glycoprotein Complex-Encoding Retroviral Vector for Isolating Resistant Variants to Inhibitors in BSL-2.
    Article Snippet: To detect the LASV GPC, the membrane was incubated with a 1/500- diluted mouse anti-LASV GP1 monoclonal antibody EBS-I-301 (PROGEN, Heidelberg, Germany) followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody (Bio-Rad). .. For detection of actin protein as a loading control, the membrane was incubated with a 1/2000-diluted mouse anti-actin antibody sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody. .. Finally, antibody-bound proteins were visualized using an HRP substrate (Bio-Rad) by C-DiGit scanner (LI-COR, Lincoln, NB, USA).

    Article Title: Development of a Conditional Replication System Using a Lassa Virus Glycoprotein Complex-Encoding Retroviral Vector for Isolating Resistant Variants to Inhibitors in BSL-2
    Article Snippet: To detect the LASV GPC, the membrane was incubated with a 1/500-diluted mouse anti-LASV GP1 monoclonal antibody EBS-I-301 (PROGEN, Heidelberg, Germany) followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody (Bio-Rad). .. For detection of actin protein as a loading control, the membrane was incubated with a 1/2000-diluted mouse anti-actin antibody sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody. .. Finally, antibody-bound proteins were visualized using an HRP substrate (Bio-Rad) by C-DiGit scanner (LI-COR, Lincoln, NB, USA).

    Incubation:

    Article Title: Development of a Conditional Replication System Using a Lassa Virus Glycoprotein Complex-Encoding Retroviral Vector for Isolating Resistant Variants to Inhibitors in BSL-2.
    Article Snippet: To detect the LASV GPC, the membrane was incubated with a 1/500- diluted mouse anti-LASV GP1 monoclonal antibody EBS-I-301 (PROGEN, Heidelberg, Germany) followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody (Bio-Rad). .. For detection of actin protein as a loading control, the membrane was incubated with a 1/2000-diluted mouse anti-actin antibody sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody. .. Finally, antibody-bound proteins were visualized using an HRP substrate (Bio-Rad) by C-DiGit scanner (LI-COR, Lincoln, NB, USA).

    Article Title: A shift in the cellular redox state redirects aspartate for export under glucose deprivation.
    Article Snippet: After blocking in 5% bovine serum albumin (BSA)-TBST or milk-TBST for 1 hour at room temperature, membranes were probed with GOT1 antibody (ab239487, Abcam, Cambridge, UK; milk) 1:1000, GOT2 antibody (HPA018139, Sigma-Aldrich; milk) 1:2000, or VDAC1/2/3 mitochondrial loading control antibody (ab15895, Abcam; BSA) 1:5000. .. As a loading control, membranes were incubated with a β-actin antibody (sc-47778, clone C4, Santa Cruz Biotechnology, Dallas, TX) 1:3000 in 5% milk-TBST or with GAPDH-antibody conjugated to peroxidase (sc-365062) 1:5000 in 5% BSA-TBST. ..

    Article Title: A shift in the cellular redox state redirects aspartate for export under glucose deprivation
    Article Snippet: After blocking in 5% bovine serum albumin (BSA)-TBST or milk-TBST for 1 hour at room temperature, membranes were probed with GOT1 antibody (ab239487, Abcam, Cambridge, UK; milk) 1:1000, GOT2 antibody (HPA018139, Sigma-Aldrich; milk) 1:2000, or VDAC1/2/3 mitochondrial loading control antibody (ab15895, Abcam; BSA) 1:5000. .. As a loading control, membranes were incubated with a β-actin antibody (sc-47778, clone C4, Santa Cruz Biotechnology, Dallas, TX) 1:3000 in 5% milk-TBST or with GAPDH-antibody conjugated to peroxidase (sc-365062) 1:5000 in 5% BSA-TBST. ..

    Article Title: Development of a Conditional Replication System Using a Lassa Virus Glycoprotein Complex-Encoding Retroviral Vector for Isolating Resistant Variants to Inhibitors in BSL-2
    Article Snippet: To detect the LASV GPC, the membrane was incubated with a 1/500-diluted mouse anti-LASV GP1 monoclonal antibody EBS-I-301 (PROGEN, Heidelberg, Germany) followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody (Bio-Rad). .. For detection of actin protein as a loading control, the membrane was incubated with a 1/2000-diluted mouse anti-actin antibody sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by 1/1000-diluted HRP-conjugated anti-mouse IgG antibody. .. Finally, antibody-bound proteins were visualized using an HRP substrate (Bio-Rad) by C-DiGit scanner (LI-COR, Lincoln, NB, USA).

    Molecular Weight:

    Article Title: N -( p -Coumaroyl) Serotonin Ameliorates LPS-Induced Inflammation in BV2 Microglia via MAPK/NF-κB Inactivation and HO-1/NQO1 Upregulation.
    Article Snippet: Immunoreactive bands were visualized using an enhanced chemiluminescence (ECL) system (Thermo Fisher Scientific, Inc.). .. Densitometric quantification was subsequently carried out using ImageJ software (version 1.52a; National Institutes of Health, Bethesda, MD, USA), with β-actin serving as the internal loading control. https://doi.org/10.3390/cimb48020232 β-actin Santa Cruz Biotech (Dallas, TX, USA) #sc-47778 43 M Antibodies were used at 1:1000 dilution (1:5000 for β-actin). p-, phosphorylated; MW, molecular weight; R, rabbit; M, mouse. ..

    Western Blot:

    Article Title: Upregulation of a CFTR mRNA isoform has therapeutic potential for the treatment of 3′ CFTR PTC variants
    Article Snippet: .. Western blot analyses were performed as described previously, except the loading control, ACTB was detected with beta actin antibody C4 (sc-47778) from Santa Cruz Biotechnology. .. Blots were imaged using the ChemiDoc MP Imaging System (Bio-Rad) or ChemiDoc XRS+ Gel Documentation System (Bio-Rad) and analyzed with the accompanying image analysis software.



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