Journal: Frontiers in Oncology

Article Title: Transforming Growth Factor-Beta-Regulated LncRNA-MUF Promotes Invasion by Modulating the miR-34a Snail1 Axis in Glioblastoma Multiforme

doi: 10.3389/fonc.2021.788755

Figure Lengend Snippet: LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated lncRNAs identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.

Article Snippet: Agilent SurePrint G3 Gene Expression Microarrays for Human (v3) for lncRNAs, containing 30,606 lncRNAs and 37,756 RefSeq-coding transcripts, were used to interrogate lncRNA and mRNA changes in vehicle versus TGF-β1 (10 ng/ml)-treated T98G cells after 24 h. RNA was isolated using MN NucleoSpin RNA Plus isolation kit (Cat. No. 740984.5).

Techniques: Genome Wide, Microarray, Quantitative RT-PCR, Expressing, Mutagenesis