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lncrna+mrna human gene expression microarray v3.0,4 × 180k  (Agilent technologies)


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    Agilent technologies lncrna+mrna human gene expression microarray v3.0,4 × 180k
    Lncrna+Mrna Human Gene Expression Microarray V3.0,4 × 180k, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lncrna+gene+expression+microarray/pmc09243442-3-16-8
    Average 90 stars, based on 1 article reviews
    lncrna+mrna human gene expression microarray v3.0,4 × 180k - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Abstracts from the 56 th European Society of Human Genetics (ESHG) Conference: e-Posters.
    Article Snippet: Genetic investigation was performed using 180K Agilent oligonucleotides array CGH analysis according to the manufacturer’s instructions.

    Article Title: KDM1A genotyping and expression in 146 sporadic somatotroph pituitary adenomas.
    Article Snippet: Importance: A paradoxical increase of growth hormone (GH) following oral glucose load has been described in ∼30% of patients with acromegaly and has been related to the ectopic expression of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) in somatotropinomas.. Recently, we identified germline pathogenic variants and somatic loss of heterozygosity of lysine demethylase 1A (KDM1A) in patients with GIP-dependent primary bilateral macronodular adrenal hyperplasia with Cushing's syndrome.. The ectopic expression of GIPR in both adrenal and pituitary lesions suggests a common molecular mechanism.

    Article Title: Clinical and molecular cytogenetic studies of five new patients with 20q11q12 deletion and review of the literature: Proposition of two critical regions.
    Article Snippet: It was conducted using oligo 60k Agilent® array for Patients #1, #2, and #3, and oligo 180k Agilent® array (Agilent technologies, Santa, Clara, CA, USA) for Patient #5.

    Microarray:

    Article Title: Phenotypic spectrum in Weiss-Kruszka syndrome caused by ZNF462 variants: Three new patients and literature review.
    Article Snippet: .. Array-CGH microarray utilized peripheral blood and 180k microarray slides from Agilent Technologies, following the manufacturer’s guidelines. .. Scanned images underwent processing through Agilent Genomic Workbench version 6.5 and Cartagenia software (Agilent Technologies).

    Article Title: Amplification of Mutant NRAS in Melanocytic Tumors With Features of Spitz Tumors.
    Article Snippet: .. For 5 cases, copy number variation was evaluated by array comparative genomic hybridization (CGH) on Agilent SurePrint G3 Human CGH Microarray 4 180K arrays with commercially available normal reference DNA. ..

    Article Title: DNA sequence and chromatin differentiate sequence-specific transcription factor binding in the human malaria parasite Plasmodium falciparum.
    Article Snippet: .. Overall, the total number of dsDNA probes was 174 550 spots, which were arrayed using an Agilent Technologies 4 × 180k microarray design. .. Overall, the total number of dsDNA probes was 174 550 spots, which were arrayed using an Agilent Technologies 4 × 180k microarray design.

    Article Title: DNA sequence and chromatin differentiate sequence-specific transcription factor binding in the human malaria parasite Plasmodium falciparum.
    Article Snippet: .. Additionally, each DNA probe was replicated in random areas of the microarray surface (four C AC AC A / GTGC AC replicates and three GT AC / TGCA TGCA replicates per orientation), which brought the total number of DNA probes to 174,550 spots for a 4 × 180k microarray (Agilent Technologies). ..

    Article Title: Novel PUF60 variant suggesting an interaction between Verheij and Cornelia de Lange syndrome: phenotype description and review of the literature
    Article Snippet: .. Array-CGH microarray involved the use of peripheral blood and 180k microarray slides from Agilent Technologies (Santa Clara, CA), following the manufacturer’s protocols. .. Scanned images were processed using Agilent Genomic Workbench version 6.5 and Cartagenia software (Agilent Technologies).

    Hybridization:

    Article Title: Amplification of Mutant NRAS in Melanocytic Tumors With Features of Spitz Tumors.
    Article Snippet: .. For 5 cases, copy number variation was evaluated by array comparative genomic hybridization (CGH) on Agilent SurePrint G3 Human CGH Microarray 4 180K arrays with commercially available normal reference DNA. ..



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    Agilent technologies sureprint g3 gene expression microarrays human (v3) lncrnas
    LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated <t>lncRNAs</t> identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.
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    Image Search Results


    LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated lncRNAs identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.

    Journal: Frontiers in Oncology

    Article Title: Transforming Growth Factor-Beta-Regulated LncRNA-MUF Promotes Invasion by Modulating the miR-34a Snail1 Axis in Glioblastoma Multiforme

    doi: 10.3389/fonc.2021.788755

    Figure Lengend Snippet: LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated lncRNAs identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.

    Article Snippet: Agilent SurePrint G3 Gene Expression Microarrays for Human (v3) for lncRNAs, containing 30,606 lncRNAs and 37,756 RefSeq-coding transcripts, were used to interrogate lncRNA and mRNA changes in vehicle versus TGF-β1 (10 ng/ml)-treated T98G cells after 24 h. RNA was isolated using MN NucleoSpin RNA Plus isolation kit (Cat. No. 740984.5).

    Techniques: Genome Wide, Microarray, Quantitative RT-PCR, Expressing, Mutagenesis