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leukemia inhibitory factor (R&D Systems)

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R&D Systems leukemia inhibitory factor
Leukemia Inhibitory Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lif+protein/Recombinant+Human+LIF+Protein/pm42822105-87-55-59
Average 93 stars, based on 65 article reviews

leukemia inhibitory factor - by Bioz Stars, 2026-11

93/100 stars

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Double Staining:

Article Title: Studies using leukemia inhibitory factor (LIF) knockout mice and a LIF adenoviral vector demonstrate a key anti-inflammatory role for this cytokine in cutaneous inflammation.
Article Snippet: Following immersion in 5-bromo-4-chloro-3-indolyl b-D-galactoside (X-Gal) reagent (5 mM K3Fe(CN)6, 5 mM K4Fe(CN)6, 25 mM MgCl2, and 2 mg/ml X-Gal; Roche Molecular Biochemicals, Indianapolis, IN) for 4 h at 37°C, sections were counterstained with eosin and examined by light microscopy. .. Double staining for LIF protein and b-galactosidase Sections stained with X-Gal reagent were blocked with 2% swine serum for 2 h at room temperature (RT) and then immunostained overnight at 4°C with polyclonal goat anti-LIF Abs (R&D Systems, Minneapolis, MN) at a 1:2000 dilution in 2% swine serum in PBS. .. Biotinylated swine anti-goat IgG (Roche) was then applied for 2 h at RT.

Staining:

Cell Culture:

Article Title: Protein Kinase Inhibitor γ reciprocally regulates osteoblast and adipocyte differentiation by downregulating Leukemia Inhibitory Factor
Article Snippet: .. LIF protein in cell culture supernatants was measured using a kit (MLF00, R & D Systems) following the recommendations of the manufacturer. .. To determine whether LIF mediates the effects of Pkig deletion or knockdown, indicated concentrations of either a neutralizing affinity-purified goat anti-murine LIF antibody (AF449, R & D Systems, Minneapolis, MN) or a normal goat control immunoglobulin (AB-108-C, R & D Systems) was incubated with MEFs or mdMSCs experiments.