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mouse leydig cell line tm3 678  (ATCC)


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    ATCC mouse leydig cell line tm3 678
    Mouse Leydig Cell Line Tm3 678, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/leydig+cells/TM3/pm42167485-290-20-31
    Average 95 stars, based on 319 article reviews
    mouse leydig cell line tm3 678 - by Bioz Stars, 2026-09
    95/100 stars

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    Cell Culture:

    Article Title: THE POTENTIAL EFFECTS OF TRIBULUS TERRESTRIS L. ON CELLULAR PARAMETERS AND STEROIDOGENESIS IN VITRO
    Article Snippet: All obtained data were collected and processed using the Agilent OpenLab ChemStation software for LC 3D Systems (Luksic et al., 2016). .. Cell culture and in vitro setups The mice TM3 line of Leydig cells (provided by American Type Culture Collection, ATCC #CRL-1714TM; Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture (Ham’s) F12 along with HEPEs with phenol red (DMEM/F12; Sigma-Aldrich, St. Louis, MO, USA). .. The medium was enhanced with 2.5% fetal bovine serum (FBS; BiochromAG, Berlin, Germany), 5% horse serum (HS; Gibco-Life Technologies, Auckland, New Zealand), 2.5 mM L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), 1% penicillin and streptomycin formula (Sigma-Aldrich, St. Louis, MO, USA) and maintained at 37°C, 5% CO2 under the 95% humidified atmosphere.

    Article Title: Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions.
    Article Snippet: 293T cells (CRL-3216; ATCC) and 3T3-L1 preadipocytes (CL-173; ATCC) were cultured in high-glucose DMEM (Life Technologies) D ow nloaded from http://rupress.org/jcb/article-pdf/217/3/975/1600317/jcb_201704184.pdf by guest on 13 D ecem ber 2024 rab18-NrZ-SNA re promotes lipid droplet growth • Xu et al. 991 containing 10% FBS (Life Technologies), 2 mM l-glutamine, 100 U/ ml penicillin, and 100 μg/ml streptomycin at 37°C in a humidified incubator containing 5% CO2. .. TM-3 Leydig cells were obtained from ATCC (CRL-1714) and cultured in high-glucose DMEM supplemented with 5% FBS and 2.5% horse serum. .. Monolayers of 3T3-L1 preadipocytes were induced to differentiate into mature adipocytes as described by the protocol of ATCC.

    Article Title: The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
    Article Snippet: Additionally, 10 l of a 50 ng/ml testosterone standard (purchased from Sigma-Aldrich, DE) was injected into the LC-MS instrument using the same parameters, and its m/z ratio was obtained for comparison with the extracts. .. In vitro investigations of Tongkat Ali extracts in TM-3 Leydig cells The TM-3 Leydig cell line (American Type Culture Collection, ATCC® CRL-1714TM, US), isolated from mouse testes, was selected as the cultured cell type, consistent with previous work on Tongkat Ali. .. 9 The complete medium consisted of DMEM/F-12 media (Nacalai Tesque, JP), which is a 1:1 ratio of Dulbecco’s Modified Eagles Medium and F-12 Ham’s nutrient mixture, 2.5 % Fetal Bovine Serum (Thermo Scientific, UK), 5% Horse serum (Thermo Scientific, UK) and supplemented with 1% penicillin/streptomycin (Nacalai Tesque, JP) were prepared for the cell cultivation.

    Article Title: Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions
    Article Snippet: 293T cells (CRL-3216; ATCC) and 3T3-L1 preadipocytes (CL-173; ATCC) were cultured in high-glucose DMEM (Life Technologies) containing 10% FBS (Life Technologies), 2 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C in a humidified incubator containing 5% CO 2 . .. TM-3 Leydig cells were obtained from ATCC (CRL-1714) and cultured in high-glucose DMEM supplemented with 5% FBS and 2.5% horse serum. .. Monolayers of 3T3-L1 preadipocytes were induced to differentiate into mature adipocytes as described by the protocol of ATCC.

    Article Title: The AP-1 transcription factors differentially regulate Cx43 expression in a ERK1/2-dependent manner in MA-10 Leydig cells.
    Article Snippet: The expression vector for mouse Creb1 (Cat #: MR204788) was purchased from OriGene (OriGene Technologies, Inc., Rockville, MD, USA). .. Mouse MA-10 (CRL-3050TM, ATCC) and TM3 (CRL-1714TM, ATCC) Leydig cells were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured as previously described [5,15,16]. .. MA-10 and TM3 cells were transfected using the jetPRIME® reagent (Polyplus Transfection, Illkirch, France) as previously described [6].

    In Vitro:

    Article Title: THE POTENTIAL EFFECTS OF TRIBULUS TERRESTRIS L. ON CELLULAR PARAMETERS AND STEROIDOGENESIS IN VITRO
    Article Snippet: All obtained data were collected and processed using the Agilent OpenLab ChemStation software for LC 3D Systems (Luksic et al., 2016). .. Cell culture and in vitro setups The mice TM3 line of Leydig cells (provided by American Type Culture Collection, ATCC #CRL-1714TM; Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture (Ham’s) F12 along with HEPEs with phenol red (DMEM/F12; Sigma-Aldrich, St. Louis, MO, USA). .. The medium was enhanced with 2.5% fetal bovine serum (FBS; BiochromAG, Berlin, Germany), 5% horse serum (HS; Gibco-Life Technologies, Auckland, New Zealand), 2.5 mM L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), 1% penicillin and streptomycin formula (Sigma-Aldrich, St. Louis, MO, USA) and maintained at 37°C, 5% CO2 under the 95% humidified atmosphere.

    Article Title: The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
    Article Snippet: Additionally, 10 l of a 50 ng/ml testosterone standard (purchased from Sigma-Aldrich, DE) was injected into the LC-MS instrument using the same parameters, and its m/z ratio was obtained for comparison with the extracts. .. In vitro investigations of Tongkat Ali extracts in TM-3 Leydig cells The TM-3 Leydig cell line (American Type Culture Collection, ATCC® CRL-1714TM, US), isolated from mouse testes, was selected as the cultured cell type, consistent with previous work on Tongkat Ali. .. 9 The complete medium consisted of DMEM/F-12 media (Nacalai Tesque, JP), which is a 1:1 ratio of Dulbecco’s Modified Eagles Medium and F-12 Ham’s nutrient mixture, 2.5 % Fetal Bovine Serum (Thermo Scientific, UK), 5% Horse serum (Thermo Scientific, UK) and supplemented with 1% penicillin/streptomycin (Nacalai Tesque, JP) were prepared for the cell cultivation.

    Article Title: In Vitro and In Vivo Evaluations of Smilax myosotiflora (Ubi Jaga) Compared to the Well-Known Aphrodisiac Plant of Eurycoma longifolia (Tongkat Ali)
    Article Snippet: Ph Eur., Merck, US) while being shaken to reveal the protein bands. .. In vitro evaluations with testicular cell cultures Cell cultures of TM-3 Leydig cells (CRL-1714TM cell types, acquired from American Type Culture Collections, ATCC, US) were grown to confluency.13,14 Each well was approximated with 4000 cells using a hemocytometer (Neubauer, CN). .. After treating each well with 50 μg/mL of the plant extracts for 72 hours, the quantities of testosterone were measured using an ELISA kit (Elabscience Biotechnology Inc., US).

    Modification:

    Article Title: THE POTENTIAL EFFECTS OF TRIBULUS TERRESTRIS L. ON CELLULAR PARAMETERS AND STEROIDOGENESIS IN VITRO
    Article Snippet: All obtained data were collected and processed using the Agilent OpenLab ChemStation software for LC 3D Systems (Luksic et al., 2016). .. Cell culture and in vitro setups The mice TM3 line of Leydig cells (provided by American Type Culture Collection, ATCC #CRL-1714TM; Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture (Ham’s) F12 along with HEPEs with phenol red (DMEM/F12; Sigma-Aldrich, St. Louis, MO, USA). .. The medium was enhanced with 2.5% fetal bovine serum (FBS; BiochromAG, Berlin, Germany), 5% horse serum (HS; Gibco-Life Technologies, Auckland, New Zealand), 2.5 mM L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), 1% penicillin and streptomycin formula (Sigma-Aldrich, St. Louis, MO, USA) and maintained at 37°C, 5% CO2 under the 95% humidified atmosphere.

    Isolation:

    Article Title: The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
    Article Snippet: Additionally, 10 l of a 50 ng/ml testosterone standard (purchased from Sigma-Aldrich, DE) was injected into the LC-MS instrument using the same parameters, and its m/z ratio was obtained for comparison with the extracts. .. In vitro investigations of Tongkat Ali extracts in TM-3 Leydig cells The TM-3 Leydig cell line (American Type Culture Collection, ATCC® CRL-1714TM, US), isolated from mouse testes, was selected as the cultured cell type, consistent with previous work on Tongkat Ali. .. 9 The complete medium consisted of DMEM/F-12 media (Nacalai Tesque, JP), which is a 1:1 ratio of Dulbecco’s Modified Eagles Medium and F-12 Ham’s nutrient mixture, 2.5 % Fetal Bovine Serum (Thermo Scientific, UK), 5% Horse serum (Thermo Scientific, UK) and supplemented with 1% penicillin/streptomycin (Nacalai Tesque, JP) were prepared for the cell cultivation.

    Article Title: Biocompatibility of Nickel Ferrite Nanoparticles on Systemic and Testicular Cells
    Article Snippet: .. For biological assays, the following immortalized cell lines were used: hepatic cells (AML-12, ATCC CRL-2254), originating from hepatocytes isolated from the liver of a 3 month-old normal mouse; renal cells (VERO, ATCC CCL-81), derived from renal epithelial cells of an African green monkey; Leydig cells (TM3, ATCC CRL-1714), isolated from a male mouse; and germ cells (GC-1, ATCC CRL-2053), isolated from the testis of a 10 day-old male mouse. ..

    Derivative Assay:

    Article Title: Biocompatibility of Nickel Ferrite Nanoparticles on Systemic and Testicular Cells
    Article Snippet: .. For biological assays, the following immortalized cell lines were used: hepatic cells (AML-12, ATCC CRL-2254), originating from hepatocytes isolated from the liver of a 3 month-old normal mouse; renal cells (VERO, ATCC CCL-81), derived from renal epithelial cells of an African green monkey; Leydig cells (TM3, ATCC CRL-1714), isolated from a male mouse; and germ cells (GC-1, ATCC CRL-2053), isolated from the testis of a 10 day-old male mouse. ..



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